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Signal Disruption Leads to Changes in Bacterial Community Population
The disruption of bacterial signaling (quorum quenching) has been proven to be an innovative approach to influence the behavior of bacteria. In particular, lactonase enzymes that are capable of hydrolyzing the N-acyl homoserine lactone (AHL) molecules used by numerous bacteria, were reported to inhi...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449428/ https://www.ncbi.nlm.nih.gov/pubmed/30984139 http://dx.doi.org/10.3389/fmicb.2019.00611 |
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author | Schwab, Michael Bergonzi, Celine Sakkos, Jonathan Staley, Christopher Zhang, Qian Sadowsky, Michael J. Aksan, Alptekin Elias, Mikael |
author_facet | Schwab, Michael Bergonzi, Celine Sakkos, Jonathan Staley, Christopher Zhang, Qian Sadowsky, Michael J. Aksan, Alptekin Elias, Mikael |
author_sort | Schwab, Michael |
collection | PubMed |
description | The disruption of bacterial signaling (quorum quenching) has been proven to be an innovative approach to influence the behavior of bacteria. In particular, lactonase enzymes that are capable of hydrolyzing the N-acyl homoserine lactone (AHL) molecules used by numerous bacteria, were reported to inhibit biofilm formation, including those of freshwater microbial communities. However, insights and tools are currently lacking to characterize, understand and explain the effects of signal disruption on complex microbial communities. Here, we produced silica capsules containing an engineered lactonase that exhibits quorum quenching activity. Capsules were used to design a filtration cartridge to selectively degrade AHLs from a recirculating bioreactor. The growth of a complex microbial community in the bioreactor, in the presence or absence of lactonase, was monitored over a 3-week period. Dynamic population analysis revealed that signal disruption using a quorum quenching lactonase can effectively reduce biofilm formation in the recirculating bioreactor system and that biofilm inhibition is concomitant to drastic changes in the composition, diversity and abundance of soil bacterial communities within these biofilms. Effects of the quorum quenching lactonase on the suspension community also affected the microbial composition, suggesting that effects of signal disruption are not limited to biofilm populations. This unexpected finding is evidence for the importance of signaling in the competition between bacteria within communities. This study provides foundational tools and data for the investigation of the importance of AHL-based signaling in the context of complex microbial communities. |
format | Online Article Text |
id | pubmed-6449428 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-64494282019-04-12 Signal Disruption Leads to Changes in Bacterial Community Population Schwab, Michael Bergonzi, Celine Sakkos, Jonathan Staley, Christopher Zhang, Qian Sadowsky, Michael J. Aksan, Alptekin Elias, Mikael Front Microbiol Microbiology The disruption of bacterial signaling (quorum quenching) has been proven to be an innovative approach to influence the behavior of bacteria. In particular, lactonase enzymes that are capable of hydrolyzing the N-acyl homoserine lactone (AHL) molecules used by numerous bacteria, were reported to inhibit biofilm formation, including those of freshwater microbial communities. However, insights and tools are currently lacking to characterize, understand and explain the effects of signal disruption on complex microbial communities. Here, we produced silica capsules containing an engineered lactonase that exhibits quorum quenching activity. Capsules were used to design a filtration cartridge to selectively degrade AHLs from a recirculating bioreactor. The growth of a complex microbial community in the bioreactor, in the presence or absence of lactonase, was monitored over a 3-week period. Dynamic population analysis revealed that signal disruption using a quorum quenching lactonase can effectively reduce biofilm formation in the recirculating bioreactor system and that biofilm inhibition is concomitant to drastic changes in the composition, diversity and abundance of soil bacterial communities within these biofilms. Effects of the quorum quenching lactonase on the suspension community also affected the microbial composition, suggesting that effects of signal disruption are not limited to biofilm populations. This unexpected finding is evidence for the importance of signaling in the competition between bacteria within communities. This study provides foundational tools and data for the investigation of the importance of AHL-based signaling in the context of complex microbial communities. Frontiers Media S.A. 2019-03-29 /pmc/articles/PMC6449428/ /pubmed/30984139 http://dx.doi.org/10.3389/fmicb.2019.00611 Text en Copyright © 2019 Schwab, Bergonzi, Sakkos, Staley, Zhang, Sadowsky, Aksan and Elias. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Schwab, Michael Bergonzi, Celine Sakkos, Jonathan Staley, Christopher Zhang, Qian Sadowsky, Michael J. Aksan, Alptekin Elias, Mikael Signal Disruption Leads to Changes in Bacterial Community Population |
title | Signal Disruption Leads to Changes in Bacterial Community Population |
title_full | Signal Disruption Leads to Changes in Bacterial Community Population |
title_fullStr | Signal Disruption Leads to Changes in Bacterial Community Population |
title_full_unstemmed | Signal Disruption Leads to Changes in Bacterial Community Population |
title_short | Signal Disruption Leads to Changes in Bacterial Community Population |
title_sort | signal disruption leads to changes in bacterial community population |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449428/ https://www.ncbi.nlm.nih.gov/pubmed/30984139 http://dx.doi.org/10.3389/fmicb.2019.00611 |
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