Cargando…

Impacts of Duck-Origin Parvovirus Infection on Cherry Valley Ducklings From the Perspective of Gut Microbiota

Duck-origin goose parvovirus (D-GPV) is the causative agent of beak atrophy and dwarfism syndrome (BADS), characterized by growth retardation, skeletal dysplasia, and persistent diarrhea. However, the pathogenic mechanism of D-GPV remains undefined. Here, we first reported the gut microbiome diversi...

Descripción completa

Detalles Bibliográficos
Autores principales: Luo, Qihui, Xu, Jing, Huang, Chao, Lei, Xinyu, Cheng, Dongjing, Liu, Wentao, Cheng, Anchun, Tang, Li, Fang, Jing, Ou, Yangping, Geng, Yi, Chen, Zhengli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6450226/
https://www.ncbi.nlm.nih.gov/pubmed/30984145
http://dx.doi.org/10.3389/fmicb.2019.00624
_version_ 1783408998552174592
author Luo, Qihui
Xu, Jing
Huang, Chao
Lei, Xinyu
Cheng, Dongjing
Liu, Wentao
Cheng, Anchun
Tang, Li
Fang, Jing
Ou, Yangping
Geng, Yi
Chen, Zhengli
author_facet Luo, Qihui
Xu, Jing
Huang, Chao
Lei, Xinyu
Cheng, Dongjing
Liu, Wentao
Cheng, Anchun
Tang, Li
Fang, Jing
Ou, Yangping
Geng, Yi
Chen, Zhengli
author_sort Luo, Qihui
collection PubMed
description Duck-origin goose parvovirus (D-GPV) is the causative agent of beak atrophy and dwarfism syndrome (BADS), characterized by growth retardation, skeletal dysplasia, and persistent diarrhea. However, the pathogenic mechanism of D-GPV remains undefined. Here, we first reported the gut microbiome diversity of D-GPV infected Cherry Valley ducks. In the investigation for the influence of D-GPV infection on gut microbiota through a period of infection, we found that D-GPV infection caused gut microbiota dysbiosis by reducing the prevalence of the dominant genera and decreasing microbial diversity. Furthermore, exfoliation of the intestinal epithelium, proliferation of lymphocytes, up-regulated mRNA expression of pro-inflammatory TNF-α, IL-1β, IL-6, IL-17A, and IL-22 and down-regulated mRNA expression of anti-inflammatory IL-10 and IL-4 occurred when D-GPV targeted in cecal epithelium. In addition, the content of short chain fatty acids (SCFAs) in cecal contents was significantly reduced after D-GPV infection. Importantly, the disorder of pro-inflammatory and anti-inflammatory cytokines was associated with the decrease of SCFAs-producing bacteria and the enrichment of opportunistic pathogens. Collectively, the decrease of SCFAs and the enrichment of pathogen-containing gut communities promoted intestinal inflammatory injury. These results may provide a new insight that target the gut microbiota to understand the progression of BADS disease and to research the pathogenic mechanism of D-GPV.
format Online
Article
Text
id pubmed-6450226
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-64502262019-04-12 Impacts of Duck-Origin Parvovirus Infection on Cherry Valley Ducklings From the Perspective of Gut Microbiota Luo, Qihui Xu, Jing Huang, Chao Lei, Xinyu Cheng, Dongjing Liu, Wentao Cheng, Anchun Tang, Li Fang, Jing Ou, Yangping Geng, Yi Chen, Zhengli Front Microbiol Microbiology Duck-origin goose parvovirus (D-GPV) is the causative agent of beak atrophy and dwarfism syndrome (BADS), characterized by growth retardation, skeletal dysplasia, and persistent diarrhea. However, the pathogenic mechanism of D-GPV remains undefined. Here, we first reported the gut microbiome diversity of D-GPV infected Cherry Valley ducks. In the investigation for the influence of D-GPV infection on gut microbiota through a period of infection, we found that D-GPV infection caused gut microbiota dysbiosis by reducing the prevalence of the dominant genera and decreasing microbial diversity. Furthermore, exfoliation of the intestinal epithelium, proliferation of lymphocytes, up-regulated mRNA expression of pro-inflammatory TNF-α, IL-1β, IL-6, IL-17A, and IL-22 and down-regulated mRNA expression of anti-inflammatory IL-10 and IL-4 occurred when D-GPV targeted in cecal epithelium. In addition, the content of short chain fatty acids (SCFAs) in cecal contents was significantly reduced after D-GPV infection. Importantly, the disorder of pro-inflammatory and anti-inflammatory cytokines was associated with the decrease of SCFAs-producing bacteria and the enrichment of opportunistic pathogens. Collectively, the decrease of SCFAs and the enrichment of pathogen-containing gut communities promoted intestinal inflammatory injury. These results may provide a new insight that target the gut microbiota to understand the progression of BADS disease and to research the pathogenic mechanism of D-GPV. Frontiers Media S.A. 2019-03-28 /pmc/articles/PMC6450226/ /pubmed/30984145 http://dx.doi.org/10.3389/fmicb.2019.00624 Text en Copyright © 2019 Luo, Xu, Huang, Lei, Cheng, Liu, Cheng, Tang, Fang, Ou, Geng and Chen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Luo, Qihui
Xu, Jing
Huang, Chao
Lei, Xinyu
Cheng, Dongjing
Liu, Wentao
Cheng, Anchun
Tang, Li
Fang, Jing
Ou, Yangping
Geng, Yi
Chen, Zhengli
Impacts of Duck-Origin Parvovirus Infection on Cherry Valley Ducklings From the Perspective of Gut Microbiota
title Impacts of Duck-Origin Parvovirus Infection on Cherry Valley Ducklings From the Perspective of Gut Microbiota
title_full Impacts of Duck-Origin Parvovirus Infection on Cherry Valley Ducklings From the Perspective of Gut Microbiota
title_fullStr Impacts of Duck-Origin Parvovirus Infection on Cherry Valley Ducklings From the Perspective of Gut Microbiota
title_full_unstemmed Impacts of Duck-Origin Parvovirus Infection on Cherry Valley Ducklings From the Perspective of Gut Microbiota
title_short Impacts of Duck-Origin Parvovirus Infection on Cherry Valley Ducklings From the Perspective of Gut Microbiota
title_sort impacts of duck-origin parvovirus infection on cherry valley ducklings from the perspective of gut microbiota
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6450226/
https://www.ncbi.nlm.nih.gov/pubmed/30984145
http://dx.doi.org/10.3389/fmicb.2019.00624
work_keys_str_mv AT luoqihui impactsofduckoriginparvovirusinfectiononcherryvalleyducklingsfromtheperspectiveofgutmicrobiota
AT xujing impactsofduckoriginparvovirusinfectiononcherryvalleyducklingsfromtheperspectiveofgutmicrobiota
AT huangchao impactsofduckoriginparvovirusinfectiononcherryvalleyducklingsfromtheperspectiveofgutmicrobiota
AT leixinyu impactsofduckoriginparvovirusinfectiononcherryvalleyducklingsfromtheperspectiveofgutmicrobiota
AT chengdongjing impactsofduckoriginparvovirusinfectiononcherryvalleyducklingsfromtheperspectiveofgutmicrobiota
AT liuwentao impactsofduckoriginparvovirusinfectiononcherryvalleyducklingsfromtheperspectiveofgutmicrobiota
AT chenganchun impactsofduckoriginparvovirusinfectiononcherryvalleyducklingsfromtheperspectiveofgutmicrobiota
AT tangli impactsofduckoriginparvovirusinfectiononcherryvalleyducklingsfromtheperspectiveofgutmicrobiota
AT fangjing impactsofduckoriginparvovirusinfectiononcherryvalleyducklingsfromtheperspectiveofgutmicrobiota
AT ouyangping impactsofduckoriginparvovirusinfectiononcherryvalleyducklingsfromtheperspectiveofgutmicrobiota
AT gengyi impactsofduckoriginparvovirusinfectiononcherryvalleyducklingsfromtheperspectiveofgutmicrobiota
AT chenzhengli impactsofduckoriginparvovirusinfectiononcherryvalleyducklingsfromtheperspectiveofgutmicrobiota