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The involvement of perivascular spaces or tissues in the facial intradermal brain-targeted delivery
Our previous work indicates the lymphatic network and perivascular spaces or tissues might be involved in the facial intradermal brain-targeted delivery of Evans blue (EB). In this article, we presented the detailed involvement of both, and the linkage between lymphatic network and perivascular spac...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6450571/ https://www.ncbi.nlm.nih.gov/pubmed/30929532 http://dx.doi.org/10.1080/10717544.2019.1587044 |
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author | Yang, Wei Jin, Bing-Hui Chen, Ya-Jing Cao, Chang Zhu, Jia-Zhen Zhao, Ying-Zheng Yu, Xi-Chong Li, Fan-Zhu |
author_facet | Yang, Wei Jin, Bing-Hui Chen, Ya-Jing Cao, Chang Zhu, Jia-Zhen Zhao, Ying-Zheng Yu, Xi-Chong Li, Fan-Zhu |
author_sort | Yang, Wei |
collection | PubMed |
description | Our previous work indicates the lymphatic network and perivascular spaces or tissues might be involved in the facial intradermal brain-targeted delivery of Evans blue (EB). In this article, we presented the detailed involvement of both, and the linkage between lymphatic network and perivascular spaces or tissues. The in-vivo imaging, the trigeminal transection and immunohistochemistry were used. In-vivo imaging indicated intradermal injection in the mystacial pad (i.d.) delivered EB into the brain at 2-, 6- and 24 h, while intranasal injection (i.n.) delivered EB into the rostral head and intravenous injection (i.v.) diffused EB weakly into the brain. Trigeminal perineurial and epineurial EB occurred along the perivascular spaces or tissues and along brain vessels. EB diffused into the lymphatic vessels and submandibular lymph nodes. Moreover, perineurial and epineurial EB co-located or overlaid with Lyve1 immuno-reactivity and VEGF antibody, and lymphatic network connected with perivascular spaces or tissues, suggesting lymphatic system-perivascular spaces might involve in the EB delivery with i.d. The trigeminal transection reduced the trigeminal epineurial and perineurial EB and brain EB along vessels. EB diffused in the fasciculus and the perineurium, blood and lymphatic vessels in the mystacial pad, mystacial EB overlaid VEGF or Lyve1 antibody. In summary, the dermal-trigeminal-brain perivascular spaces or tissues and the linkage to the lymphatic network mediated the intradermal brain-targeted delivery. |
format | Online Article Text |
id | pubmed-6450571 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-64505712019-04-15 The involvement of perivascular spaces or tissues in the facial intradermal brain-targeted delivery Yang, Wei Jin, Bing-Hui Chen, Ya-Jing Cao, Chang Zhu, Jia-Zhen Zhao, Ying-Zheng Yu, Xi-Chong Li, Fan-Zhu Drug Deliv Research Article Our previous work indicates the lymphatic network and perivascular spaces or tissues might be involved in the facial intradermal brain-targeted delivery of Evans blue (EB). In this article, we presented the detailed involvement of both, and the linkage between lymphatic network and perivascular spaces or tissues. The in-vivo imaging, the trigeminal transection and immunohistochemistry were used. In-vivo imaging indicated intradermal injection in the mystacial pad (i.d.) delivered EB into the brain at 2-, 6- and 24 h, while intranasal injection (i.n.) delivered EB into the rostral head and intravenous injection (i.v.) diffused EB weakly into the brain. Trigeminal perineurial and epineurial EB occurred along the perivascular spaces or tissues and along brain vessels. EB diffused into the lymphatic vessels and submandibular lymph nodes. Moreover, perineurial and epineurial EB co-located or overlaid with Lyve1 immuno-reactivity and VEGF antibody, and lymphatic network connected with perivascular spaces or tissues, suggesting lymphatic system-perivascular spaces might involve in the EB delivery with i.d. The trigeminal transection reduced the trigeminal epineurial and perineurial EB and brain EB along vessels. EB diffused in the fasciculus and the perineurium, blood and lymphatic vessels in the mystacial pad, mystacial EB overlaid VEGF or Lyve1 antibody. In summary, the dermal-trigeminal-brain perivascular spaces or tissues and the linkage to the lymphatic network mediated the intradermal brain-targeted delivery. Taylor & Francis 2019-03-31 /pmc/articles/PMC6450571/ /pubmed/30929532 http://dx.doi.org/10.1080/10717544.2019.1587044 Text en © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Yang, Wei Jin, Bing-Hui Chen, Ya-Jing Cao, Chang Zhu, Jia-Zhen Zhao, Ying-Zheng Yu, Xi-Chong Li, Fan-Zhu The involvement of perivascular spaces or tissues in the facial intradermal brain-targeted delivery |
title | The involvement of perivascular spaces or tissues in the facial intradermal brain-targeted delivery |
title_full | The involvement of perivascular spaces or tissues in the facial intradermal brain-targeted delivery |
title_fullStr | The involvement of perivascular spaces or tissues in the facial intradermal brain-targeted delivery |
title_full_unstemmed | The involvement of perivascular spaces or tissues in the facial intradermal brain-targeted delivery |
title_short | The involvement of perivascular spaces or tissues in the facial intradermal brain-targeted delivery |
title_sort | involvement of perivascular spaces or tissues in the facial intradermal brain-targeted delivery |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6450571/ https://www.ncbi.nlm.nih.gov/pubmed/30929532 http://dx.doi.org/10.1080/10717544.2019.1587044 |
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