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The Gag protein PEG10 binds to RNA and regulates trophoblast stem cell lineage specification
Peg10 (paternally expressed gene 10) is an imprinted gene that is essential for placental development. It is thought to derive from a Ty3-gyspy LTR (long terminal repeat) retrotransposon and retains Gag and Pol-like domains. Here we show that the Gag domain of PEG10 can promote vesicle budding simil...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6450627/ https://www.ncbi.nlm.nih.gov/pubmed/30951545 http://dx.doi.org/10.1371/journal.pone.0214110 |
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author | Abed, Mona Verschueren, Erik Budayeva, Hanna Liu, Peter Kirkpatrick, Donald S. Reja, Rohit Kummerfeld, Sarah K. Webster, Joshua D. Gierke, Sarah Reichelt, Mike Anderson, Keith R. Newman, Robert J. Roose-Girma, Merone Modrusan, Zora Pektas, Hazal Maltepe, Emin Newton, Kim Dixit, Vishva M. |
author_facet | Abed, Mona Verschueren, Erik Budayeva, Hanna Liu, Peter Kirkpatrick, Donald S. Reja, Rohit Kummerfeld, Sarah K. Webster, Joshua D. Gierke, Sarah Reichelt, Mike Anderson, Keith R. Newman, Robert J. Roose-Girma, Merone Modrusan, Zora Pektas, Hazal Maltepe, Emin Newton, Kim Dixit, Vishva M. |
author_sort | Abed, Mona |
collection | PubMed |
description | Peg10 (paternally expressed gene 10) is an imprinted gene that is essential for placental development. It is thought to derive from a Ty3-gyspy LTR (long terminal repeat) retrotransposon and retains Gag and Pol-like domains. Here we show that the Gag domain of PEG10 can promote vesicle budding similar to the HIV p24 Gag protein. Expressed in a subset of mouse endocrine organs in addition to the placenta, PEG10 was identified as a substrate of the deubiquitinating enzyme USP9X. Consistent with PEG10 having a critical role in placental development, PEG10-deficient trophoblast stem cells (TSCs) exhibited impaired differentiation into placental lineages. PEG10 expressed in wild-type, differentiating TSCs was bound to many cellular RNAs including Hbegf (Heparin-binding EGF-like growth factor), which is known to play an important role in placentation. Expression of Hbegf was reduced in PEG10-deficient TSCs suggesting that PEG10 might bind to and stabilize RNAs that are critical for normal placental development. |
format | Online Article Text |
id | pubmed-6450627 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-64506272019-04-19 The Gag protein PEG10 binds to RNA and regulates trophoblast stem cell lineage specification Abed, Mona Verschueren, Erik Budayeva, Hanna Liu, Peter Kirkpatrick, Donald S. Reja, Rohit Kummerfeld, Sarah K. Webster, Joshua D. Gierke, Sarah Reichelt, Mike Anderson, Keith R. Newman, Robert J. Roose-Girma, Merone Modrusan, Zora Pektas, Hazal Maltepe, Emin Newton, Kim Dixit, Vishva M. PLoS One Research Article Peg10 (paternally expressed gene 10) is an imprinted gene that is essential for placental development. It is thought to derive from a Ty3-gyspy LTR (long terminal repeat) retrotransposon and retains Gag and Pol-like domains. Here we show that the Gag domain of PEG10 can promote vesicle budding similar to the HIV p24 Gag protein. Expressed in a subset of mouse endocrine organs in addition to the placenta, PEG10 was identified as a substrate of the deubiquitinating enzyme USP9X. Consistent with PEG10 having a critical role in placental development, PEG10-deficient trophoblast stem cells (TSCs) exhibited impaired differentiation into placental lineages. PEG10 expressed in wild-type, differentiating TSCs was bound to many cellular RNAs including Hbegf (Heparin-binding EGF-like growth factor), which is known to play an important role in placentation. Expression of Hbegf was reduced in PEG10-deficient TSCs suggesting that PEG10 might bind to and stabilize RNAs that are critical for normal placental development. Public Library of Science 2019-04-05 /pmc/articles/PMC6450627/ /pubmed/30951545 http://dx.doi.org/10.1371/journal.pone.0214110 Text en © 2019 Abed et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Abed, Mona Verschueren, Erik Budayeva, Hanna Liu, Peter Kirkpatrick, Donald S. Reja, Rohit Kummerfeld, Sarah K. Webster, Joshua D. Gierke, Sarah Reichelt, Mike Anderson, Keith R. Newman, Robert J. Roose-Girma, Merone Modrusan, Zora Pektas, Hazal Maltepe, Emin Newton, Kim Dixit, Vishva M. The Gag protein PEG10 binds to RNA and regulates trophoblast stem cell lineage specification |
title | The Gag protein PEG10 binds to RNA and regulates trophoblast stem cell lineage specification |
title_full | The Gag protein PEG10 binds to RNA and regulates trophoblast stem cell lineage specification |
title_fullStr | The Gag protein PEG10 binds to RNA and regulates trophoblast stem cell lineage specification |
title_full_unstemmed | The Gag protein PEG10 binds to RNA and regulates trophoblast stem cell lineage specification |
title_short | The Gag protein PEG10 binds to RNA and regulates trophoblast stem cell lineage specification |
title_sort | gag protein peg10 binds to rna and regulates trophoblast stem cell lineage specification |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6450627/ https://www.ncbi.nlm.nih.gov/pubmed/30951545 http://dx.doi.org/10.1371/journal.pone.0214110 |
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