Cargando…
Tolerance of the freeze-dried mouse sperm nucleus to temperatures ranging from −196 °C to 150 °C
It has long been believed that tolerance against extreme environments is possible only for ‘lower’ groups, such as archaea, bacteria or tardigrades, and not for more ‘advanced’ species. Here, we demonstrated that the mammalian sperm nucleus also exhibited strong tolerance to cold and hot temperature...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6450870/ https://www.ncbi.nlm.nih.gov/pubmed/30952922 http://dx.doi.org/10.1038/s41598-019-42062-8 |
_version_ | 1783409083570716672 |
---|---|
author | Wakayama, Sayaka Ito, Daiyu Kamada, Yuko Yonemura, Shigenobu Ooga, Masatoshi Kishigami, Satoshi Wakayama, Teruhiko |
author_facet | Wakayama, Sayaka Ito, Daiyu Kamada, Yuko Yonemura, Shigenobu Ooga, Masatoshi Kishigami, Satoshi Wakayama, Teruhiko |
author_sort | Wakayama, Sayaka |
collection | PubMed |
description | It has long been believed that tolerance against extreme environments is possible only for ‘lower’ groups, such as archaea, bacteria or tardigrades, and not for more ‘advanced’ species. Here, we demonstrated that the mammalian sperm nucleus also exhibited strong tolerance to cold and hot temperatures. When mouse spermatozoa were freeze-dried (FD), similar to the anhydrobiosis of Tardigrades, all spermatozoa were ostensibly dead after rehydration. However, offspring were obtained from recovered FD sperm nuclei, even after repeated treatment with conditions from liquid nitrogen to room temperature. Conversely, when FD spermatozoa were heated at 95 °C, although the birth rate was decreased with increasing duration of the treatment, offspring were obtained even for FD spermatozoa that had been heat-treated for 2 h. This period was improved up to 6 h when glucose was replaced with trehalose in the freeze-drying medium, and the resistance temperature was extended up to 150 °C for short periods of treatment. Randomly selected offspring grew into healthy adults. Our results suggest that, when considering the sperm nucleus/DNA as the material that is used as a blueprint of life, rather than cell viability, a significant tolerance to extreme temperatures is present even in ‘higher’ species, such as mammals. |
format | Online Article Text |
id | pubmed-6450870 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-64508702019-04-10 Tolerance of the freeze-dried mouse sperm nucleus to temperatures ranging from −196 °C to 150 °C Wakayama, Sayaka Ito, Daiyu Kamada, Yuko Yonemura, Shigenobu Ooga, Masatoshi Kishigami, Satoshi Wakayama, Teruhiko Sci Rep Article It has long been believed that tolerance against extreme environments is possible only for ‘lower’ groups, such as archaea, bacteria or tardigrades, and not for more ‘advanced’ species. Here, we demonstrated that the mammalian sperm nucleus also exhibited strong tolerance to cold and hot temperatures. When mouse spermatozoa were freeze-dried (FD), similar to the anhydrobiosis of Tardigrades, all spermatozoa were ostensibly dead after rehydration. However, offspring were obtained from recovered FD sperm nuclei, even after repeated treatment with conditions from liquid nitrogen to room temperature. Conversely, when FD spermatozoa were heated at 95 °C, although the birth rate was decreased with increasing duration of the treatment, offspring were obtained even for FD spermatozoa that had been heat-treated for 2 h. This period was improved up to 6 h when glucose was replaced with trehalose in the freeze-drying medium, and the resistance temperature was extended up to 150 °C for short periods of treatment. Randomly selected offspring grew into healthy adults. Our results suggest that, when considering the sperm nucleus/DNA as the material that is used as a blueprint of life, rather than cell viability, a significant tolerance to extreme temperatures is present even in ‘higher’ species, such as mammals. Nature Publishing Group UK 2019-04-05 /pmc/articles/PMC6450870/ /pubmed/30952922 http://dx.doi.org/10.1038/s41598-019-42062-8 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Wakayama, Sayaka Ito, Daiyu Kamada, Yuko Yonemura, Shigenobu Ooga, Masatoshi Kishigami, Satoshi Wakayama, Teruhiko Tolerance of the freeze-dried mouse sperm nucleus to temperatures ranging from −196 °C to 150 °C |
title | Tolerance of the freeze-dried mouse sperm nucleus to temperatures ranging from −196 °C to 150 °C |
title_full | Tolerance of the freeze-dried mouse sperm nucleus to temperatures ranging from −196 °C to 150 °C |
title_fullStr | Tolerance of the freeze-dried mouse sperm nucleus to temperatures ranging from −196 °C to 150 °C |
title_full_unstemmed | Tolerance of the freeze-dried mouse sperm nucleus to temperatures ranging from −196 °C to 150 °C |
title_short | Tolerance of the freeze-dried mouse sperm nucleus to temperatures ranging from −196 °C to 150 °C |
title_sort | tolerance of the freeze-dried mouse sperm nucleus to temperatures ranging from −196 °c to 150 °c |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6450870/ https://www.ncbi.nlm.nih.gov/pubmed/30952922 http://dx.doi.org/10.1038/s41598-019-42062-8 |
work_keys_str_mv | AT wakayamasayaka toleranceofthefreezedriedmousespermnucleustotemperaturesrangingfrom196cto150c AT itodaiyu toleranceofthefreezedriedmousespermnucleustotemperaturesrangingfrom196cto150c AT kamadayuko toleranceofthefreezedriedmousespermnucleustotemperaturesrangingfrom196cto150c AT yonemurashigenobu toleranceofthefreezedriedmousespermnucleustotemperaturesrangingfrom196cto150c AT oogamasatoshi toleranceofthefreezedriedmousespermnucleustotemperaturesrangingfrom196cto150c AT kishigamisatoshi toleranceofthefreezedriedmousespermnucleustotemperaturesrangingfrom196cto150c AT wakayamateruhiko toleranceofthefreezedriedmousespermnucleustotemperaturesrangingfrom196cto150c |