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Influence of the static magnetic field on cell response in a miniaturized optically accessible bioreactor for 3D cell culture

Hydraulic sealing is a crucial condition for the maintenance of sterility during long term operation of microfluidic bioreactors. We developed a miniaturized optically accessible bioreactor (MOAB) allowing perfused culture of 3D cellularised constructs. In the MOAB, the culture chambers are sealed b...

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Autores principales: Izzo, Luca, Tunesi, Marta, Boeri, Lucia, Laganà, Matteo, Giordano, Carmen, Raimondi, Manuela Teresa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6451746/
https://www.ncbi.nlm.nih.gov/pubmed/30868253
http://dx.doi.org/10.1007/s10544-019-0387-8
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author Izzo, Luca
Tunesi, Marta
Boeri, Lucia
Laganà, Matteo
Giordano, Carmen
Raimondi, Manuela Teresa
author_facet Izzo, Luca
Tunesi, Marta
Boeri, Lucia
Laganà, Matteo
Giordano, Carmen
Raimondi, Manuela Teresa
author_sort Izzo, Luca
collection PubMed
description Hydraulic sealing is a crucial condition for the maintenance of sterility during long term operation of microfluidic bioreactors. We developed a miniaturized optically accessible bioreactor (MOAB) allowing perfused culture of 3D cellularised constructs. In the MOAB, the culture chambers are sealed by magnets that generate a weak static magnetic field (SMF). Here, we predicted computationally the exact level of SMF to which cells are subjected during culture in the MOAB and we assessed its influence on the viability, metabolic activity and gene expression of neuroblastoma-derived cells cultured up to seven days. The predicted SMF ranged from 0.32 to 0.57 T using an axial-symmetric model of a single chamber, whereas it ranged from 0.35 to 0.62 T using a 3D model of the complete device. Cell function was evaluated in SH-SY5Y neuroblastoma cells at 2 and 7 days of culture in the MOAB, compared to 2D monolayer, 3D non-perfused constructs, and 3D perfused constructs cultured in a modified MOAB with magnet-free sealing. We measured the cell metabolic activity normalized by the DNA content and the expression levels of heat-shock protein 70 (Hsp-70), Bcl-2 and Bax. We found that the level of SMF applied to cells in the MOAB did not influence their metabolic activity and exerted a stressful effect in 2D monolayer, not confirmed in 3D conditions, neither static not perfused. Instead, the magnets provided a significantly greater hydraulic sealing in long-term culture, thus the MOAB might be potentially exploitable for the development of reliable in vitro models of neurodegeneration. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10544-019-0387-8) contains supplementary material, which is available to authorized users.
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spelling pubmed-64517462019-04-23 Influence of the static magnetic field on cell response in a miniaturized optically accessible bioreactor for 3D cell culture Izzo, Luca Tunesi, Marta Boeri, Lucia Laganà, Matteo Giordano, Carmen Raimondi, Manuela Teresa Biomed Microdevices Article Hydraulic sealing is a crucial condition for the maintenance of sterility during long term operation of microfluidic bioreactors. We developed a miniaturized optically accessible bioreactor (MOAB) allowing perfused culture of 3D cellularised constructs. In the MOAB, the culture chambers are sealed by magnets that generate a weak static magnetic field (SMF). Here, we predicted computationally the exact level of SMF to which cells are subjected during culture in the MOAB and we assessed its influence on the viability, metabolic activity and gene expression of neuroblastoma-derived cells cultured up to seven days. The predicted SMF ranged from 0.32 to 0.57 T using an axial-symmetric model of a single chamber, whereas it ranged from 0.35 to 0.62 T using a 3D model of the complete device. Cell function was evaluated in SH-SY5Y neuroblastoma cells at 2 and 7 days of culture in the MOAB, compared to 2D monolayer, 3D non-perfused constructs, and 3D perfused constructs cultured in a modified MOAB with magnet-free sealing. We measured the cell metabolic activity normalized by the DNA content and the expression levels of heat-shock protein 70 (Hsp-70), Bcl-2 and Bax. We found that the level of SMF applied to cells in the MOAB did not influence their metabolic activity and exerted a stressful effect in 2D monolayer, not confirmed in 3D conditions, neither static not perfused. Instead, the magnets provided a significantly greater hydraulic sealing in long-term culture, thus the MOAB might be potentially exploitable for the development of reliable in vitro models of neurodegeneration. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10544-019-0387-8) contains supplementary material, which is available to authorized users. Springer US 2019-03-13 2019 /pmc/articles/PMC6451746/ /pubmed/30868253 http://dx.doi.org/10.1007/s10544-019-0387-8 Text en © The Author(s) 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Article
Izzo, Luca
Tunesi, Marta
Boeri, Lucia
Laganà, Matteo
Giordano, Carmen
Raimondi, Manuela Teresa
Influence of the static magnetic field on cell response in a miniaturized optically accessible bioreactor for 3D cell culture
title Influence of the static magnetic field on cell response in a miniaturized optically accessible bioreactor for 3D cell culture
title_full Influence of the static magnetic field on cell response in a miniaturized optically accessible bioreactor for 3D cell culture
title_fullStr Influence of the static magnetic field on cell response in a miniaturized optically accessible bioreactor for 3D cell culture
title_full_unstemmed Influence of the static magnetic field on cell response in a miniaturized optically accessible bioreactor for 3D cell culture
title_short Influence of the static magnetic field on cell response in a miniaturized optically accessible bioreactor for 3D cell culture
title_sort influence of the static magnetic field on cell response in a miniaturized optically accessible bioreactor for 3d cell culture
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6451746/
https://www.ncbi.nlm.nih.gov/pubmed/30868253
http://dx.doi.org/10.1007/s10544-019-0387-8
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