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Production of Japanese Black calves by the transfer of embryos developed from in vitro-fertilized oocytes derived by ovum pick up and matured in culture with the mitogen-activated protein kinase kinase inhibitor U0126
This study investigated whether treatment with the mitogen-activated protein kinase kinase inhibitor U0126 during in vitro maturation (IVM), which has previously been reported to improve oocyte developmental competence, is practical for use in calf production using ovum pick up (OPU)-derived oocytes...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Japanese Society of Veterinary Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6451900/ https://www.ncbi.nlm.nih.gov/pubmed/30700676 http://dx.doi.org/10.1292/jvms.18-0460 |
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author | SAKAGAMI, Nobutada KONDA, Kunitoshi HASHIMURA, Shinji KAWATE, Noritoshi INABA, Toshio TAMADA, Hiromichi |
author_facet | SAKAGAMI, Nobutada KONDA, Kunitoshi HASHIMURA, Shinji KAWATE, Noritoshi INABA, Toshio TAMADA, Hiromichi |
author_sort | SAKAGAMI, Nobutada |
collection | PubMed |
description | This study investigated whether treatment with the mitogen-activated protein kinase kinase inhibitor U0126 during in vitro maturation (IVM), which has previously been reported to improve oocyte developmental competence, is practical for use in calf production using ovum pick up (OPU)-derived oocytes. Two Japanese Black cows were repeatedly and simultaneously treated to stimulate follicular growth and were prepared for OPU. Cumulus-oocyte complexes (COCs) were collected from one cow using a collection medium containing 5 µM U0126 and were cultured in medium supplemented with the same concentration of U0126 for the first 2 hr of IVM; COCs from the other cow were used as controls without U0126 treatment. The cows were exchanged between the two groups at every sequential OPU (n=8). The number of oocytes developing to blastocysts in the U0126-treated group (39.1%, 34/87) was significantly higher than that in the control group (22.1%, 19/86). Eight blastocysts produced with U0126 treatment were transferred to recipients, and four normal calves were obtained. The results indicate that embryos develop efficiently from OPU-derived oocytes treated with U0126, and that these embryos may be of practical use in calf production. |
format | Online Article Text |
id | pubmed-6451900 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Japanese Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-64519002019-04-10 Production of Japanese Black calves by the transfer of embryos developed from in vitro-fertilized oocytes derived by ovum pick up and matured in culture with the mitogen-activated protein kinase kinase inhibitor U0126 SAKAGAMI, Nobutada KONDA, Kunitoshi HASHIMURA, Shinji KAWATE, Noritoshi INABA, Toshio TAMADA, Hiromichi J Vet Med Sci Theriogenology This study investigated whether treatment with the mitogen-activated protein kinase kinase inhibitor U0126 during in vitro maturation (IVM), which has previously been reported to improve oocyte developmental competence, is practical for use in calf production using ovum pick up (OPU)-derived oocytes. Two Japanese Black cows were repeatedly and simultaneously treated to stimulate follicular growth and were prepared for OPU. Cumulus-oocyte complexes (COCs) were collected from one cow using a collection medium containing 5 µM U0126 and were cultured in medium supplemented with the same concentration of U0126 for the first 2 hr of IVM; COCs from the other cow were used as controls without U0126 treatment. The cows were exchanged between the two groups at every sequential OPU (n=8). The number of oocytes developing to blastocysts in the U0126-treated group (39.1%, 34/87) was significantly higher than that in the control group (22.1%, 19/86). Eight blastocysts produced with U0126 treatment were transferred to recipients, and four normal calves were obtained. The results indicate that embryos develop efficiently from OPU-derived oocytes treated with U0126, and that these embryos may be of practical use in calf production. The Japanese Society of Veterinary Science 2019-01-29 2019-03 /pmc/articles/PMC6451900/ /pubmed/30700676 http://dx.doi.org/10.1292/jvms.18-0460 Text en ©2019 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Theriogenology SAKAGAMI, Nobutada KONDA, Kunitoshi HASHIMURA, Shinji KAWATE, Noritoshi INABA, Toshio TAMADA, Hiromichi Production of Japanese Black calves by the transfer of embryos developed from in vitro-fertilized oocytes derived by ovum pick up and matured in culture with the mitogen-activated protein kinase kinase inhibitor U0126 |
title | Production of Japanese Black calves by the transfer of embryos developed from in vitro-fertilized oocytes derived by ovum pick up and matured in culture with
the mitogen-activated protein kinase kinase inhibitor U0126 |
title_full | Production of Japanese Black calves by the transfer of embryos developed from in vitro-fertilized oocytes derived by ovum pick up and matured in culture with
the mitogen-activated protein kinase kinase inhibitor U0126 |
title_fullStr | Production of Japanese Black calves by the transfer of embryos developed from in vitro-fertilized oocytes derived by ovum pick up and matured in culture with
the mitogen-activated protein kinase kinase inhibitor U0126 |
title_full_unstemmed | Production of Japanese Black calves by the transfer of embryos developed from in vitro-fertilized oocytes derived by ovum pick up and matured in culture with
the mitogen-activated protein kinase kinase inhibitor U0126 |
title_short | Production of Japanese Black calves by the transfer of embryos developed from in vitro-fertilized oocytes derived by ovum pick up and matured in culture with
the mitogen-activated protein kinase kinase inhibitor U0126 |
title_sort | production of japanese black calves by the transfer of embryos developed from in vitro-fertilized oocytes derived by ovum pick up and matured in culture with
the mitogen-activated protein kinase kinase inhibitor u0126 |
topic | Theriogenology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6451900/ https://www.ncbi.nlm.nih.gov/pubmed/30700676 http://dx.doi.org/10.1292/jvms.18-0460 |
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