Cargando…

Production of Japanese Black calves by the transfer of embryos developed from in vitro-fertilized oocytes derived by ovum pick up and matured in culture with the mitogen-activated protein kinase kinase inhibitor U0126

This study investigated whether treatment with the mitogen-activated protein kinase kinase inhibitor U0126 during in vitro maturation (IVM), which has previously been reported to improve oocyte developmental competence, is practical for use in calf production using ovum pick up (OPU)-derived oocytes...

Descripción completa

Detalles Bibliográficos
Autores principales: SAKAGAMI, Nobutada, KONDA, Kunitoshi, HASHIMURA, Shinji, KAWATE, Noritoshi, INABA, Toshio, TAMADA, Hiromichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6451900/
https://www.ncbi.nlm.nih.gov/pubmed/30700676
http://dx.doi.org/10.1292/jvms.18-0460
_version_ 1783409232544006144
author SAKAGAMI, Nobutada
KONDA, Kunitoshi
HASHIMURA, Shinji
KAWATE, Noritoshi
INABA, Toshio
TAMADA, Hiromichi
author_facet SAKAGAMI, Nobutada
KONDA, Kunitoshi
HASHIMURA, Shinji
KAWATE, Noritoshi
INABA, Toshio
TAMADA, Hiromichi
author_sort SAKAGAMI, Nobutada
collection PubMed
description This study investigated whether treatment with the mitogen-activated protein kinase kinase inhibitor U0126 during in vitro maturation (IVM), which has previously been reported to improve oocyte developmental competence, is practical for use in calf production using ovum pick up (OPU)-derived oocytes. Two Japanese Black cows were repeatedly and simultaneously treated to stimulate follicular growth and were prepared for OPU. Cumulus-oocyte complexes (COCs) were collected from one cow using a collection medium containing 5 µM U0126 and were cultured in medium supplemented with the same concentration of U0126 for the first 2 hr of IVM; COCs from the other cow were used as controls without U0126 treatment. The cows were exchanged between the two groups at every sequential OPU (n=8). The number of oocytes developing to blastocysts in the U0126-treated group (39.1%, 34/87) was significantly higher than that in the control group (22.1%, 19/86). Eight blastocysts produced with U0126 treatment were transferred to recipients, and four normal calves were obtained. The results indicate that embryos develop efficiently from OPU-derived oocytes treated with U0126, and that these embryos may be of practical use in calf production.
format Online
Article
Text
id pubmed-6451900
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher The Japanese Society of Veterinary Science
record_format MEDLINE/PubMed
spelling pubmed-64519002019-04-10 Production of Japanese Black calves by the transfer of embryos developed from in vitro-fertilized oocytes derived by ovum pick up and matured in culture with the mitogen-activated protein kinase kinase inhibitor U0126 SAKAGAMI, Nobutada KONDA, Kunitoshi HASHIMURA, Shinji KAWATE, Noritoshi INABA, Toshio TAMADA, Hiromichi J Vet Med Sci Theriogenology This study investigated whether treatment with the mitogen-activated protein kinase kinase inhibitor U0126 during in vitro maturation (IVM), which has previously been reported to improve oocyte developmental competence, is practical for use in calf production using ovum pick up (OPU)-derived oocytes. Two Japanese Black cows were repeatedly and simultaneously treated to stimulate follicular growth and were prepared for OPU. Cumulus-oocyte complexes (COCs) were collected from one cow using a collection medium containing 5 µM U0126 and were cultured in medium supplemented with the same concentration of U0126 for the first 2 hr of IVM; COCs from the other cow were used as controls without U0126 treatment. The cows were exchanged between the two groups at every sequential OPU (n=8). The number of oocytes developing to blastocysts in the U0126-treated group (39.1%, 34/87) was significantly higher than that in the control group (22.1%, 19/86). Eight blastocysts produced with U0126 treatment were transferred to recipients, and four normal calves were obtained. The results indicate that embryos develop efficiently from OPU-derived oocytes treated with U0126, and that these embryos may be of practical use in calf production. The Japanese Society of Veterinary Science 2019-01-29 2019-03 /pmc/articles/PMC6451900/ /pubmed/30700676 http://dx.doi.org/10.1292/jvms.18-0460 Text en ©2019 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Theriogenology
SAKAGAMI, Nobutada
KONDA, Kunitoshi
HASHIMURA, Shinji
KAWATE, Noritoshi
INABA, Toshio
TAMADA, Hiromichi
Production of Japanese Black calves by the transfer of embryos developed from in vitro-fertilized oocytes derived by ovum pick up and matured in culture with the mitogen-activated protein kinase kinase inhibitor U0126
title Production of Japanese Black calves by the transfer of embryos developed from in vitro-fertilized oocytes derived by ovum pick up and matured in culture with the mitogen-activated protein kinase kinase inhibitor U0126
title_full Production of Japanese Black calves by the transfer of embryos developed from in vitro-fertilized oocytes derived by ovum pick up and matured in culture with the mitogen-activated protein kinase kinase inhibitor U0126
title_fullStr Production of Japanese Black calves by the transfer of embryos developed from in vitro-fertilized oocytes derived by ovum pick up and matured in culture with the mitogen-activated protein kinase kinase inhibitor U0126
title_full_unstemmed Production of Japanese Black calves by the transfer of embryos developed from in vitro-fertilized oocytes derived by ovum pick up and matured in culture with the mitogen-activated protein kinase kinase inhibitor U0126
title_short Production of Japanese Black calves by the transfer of embryos developed from in vitro-fertilized oocytes derived by ovum pick up and matured in culture with the mitogen-activated protein kinase kinase inhibitor U0126
title_sort production of japanese black calves by the transfer of embryos developed from in vitro-fertilized oocytes derived by ovum pick up and matured in culture with the mitogen-activated protein kinase kinase inhibitor u0126
topic Theriogenology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6451900/
https://www.ncbi.nlm.nih.gov/pubmed/30700676
http://dx.doi.org/10.1292/jvms.18-0460
work_keys_str_mv AT sakagaminobutada productionofjapaneseblackcalvesbythetransferofembryosdevelopedfrominvitrofertilizedoocytesderivedbyovumpickupandmaturedinculturewiththemitogenactivatedproteinkinasekinaseinhibitoru0126
AT kondakunitoshi productionofjapaneseblackcalvesbythetransferofembryosdevelopedfrominvitrofertilizedoocytesderivedbyovumpickupandmaturedinculturewiththemitogenactivatedproteinkinasekinaseinhibitoru0126
AT hashimurashinji productionofjapaneseblackcalvesbythetransferofembryosdevelopedfrominvitrofertilizedoocytesderivedbyovumpickupandmaturedinculturewiththemitogenactivatedproteinkinasekinaseinhibitoru0126
AT kawatenoritoshi productionofjapaneseblackcalvesbythetransferofembryosdevelopedfrominvitrofertilizedoocytesderivedbyovumpickupandmaturedinculturewiththemitogenactivatedproteinkinasekinaseinhibitoru0126
AT inabatoshio productionofjapaneseblackcalvesbythetransferofembryosdevelopedfrominvitrofertilizedoocytesderivedbyovumpickupandmaturedinculturewiththemitogenactivatedproteinkinasekinaseinhibitoru0126
AT tamadahiromichi productionofjapaneseblackcalvesbythetransferofembryosdevelopedfrominvitrofertilizedoocytesderivedbyovumpickupandmaturedinculturewiththemitogenactivatedproteinkinasekinaseinhibitoru0126