Isolation and characterization of atypical Actinobacillus pleuropneumoniae serovar 15 lacking the apxIICA genes in Japan

Six atypical Actinobacillus pleuropneumoniae serovar 15 strains were isolated from pneumonic lesions of naturally infected dead pigs from the same farm in Japan. Genetic analyses of apx genes revealed that the atypical isolates contained the toxin-associated genes apxIBD, apxIIICA, apxIIIBD, and apx...

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Autores principales: TESHIMA, Kaho, HIRANO, Haruna, USHIYAMA, Kazutada, SHIBUYA, Kazumoto, NAGAI, Shinya, SASAKAWA, Chihiro, TO, Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2019
Materias:
Bacteriology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6451906/
https://www.ncbi.nlm.nih.gov/pubmed/30713216
http://dx.doi.org/10.1292/jvms.18-0421
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author TESHIMA, Kaho
HIRANO, Haruna
USHIYAMA, Kazutada
SHIBUYA, Kazumoto
NAGAI, Shinya
SASAKAWA, Chihiro
TO, Ho
author_facet TESHIMA, Kaho
HIRANO, Haruna
USHIYAMA, Kazutada
SHIBUYA, Kazumoto
NAGAI, Shinya
SASAKAWA, Chihiro
TO, Ho
author_sort TESHIMA, Kaho
collection PubMed
description Six atypical Actinobacillus pleuropneumoniae serovar 15 strains were isolated from pneumonic lesions of naturally infected dead pigs from the same farm in Japan. Genetic analyses of apx genes revealed that the atypical isolates contained the toxin-associated genes apxIBD, apxIIICA, apxIIIBD, and apxIVA, but not apxIICA. Coinciding with the result of the atypical gene profile, analyses of toxin protein production revealed that these atypical isolates expressed only ApxIII but not ApxII. A mouse pathogenicity test showed that the atypical isolate tested seemed to be less virulent than the typical isolates. This is the first report describing the emergence of atypical A. pleuropneumoniae serovar 15, which does not produce ApxII due to the absence of apxIICA genes, in Japan.
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spelling pubmed-64519062019-04-10 Isolation and characterization of atypical Actinobacillus pleuropneumoniae serovar 15 lacking the apxIICA genes in Japan TESHIMA, Kaho HIRANO, Haruna USHIYAMA, Kazutada SHIBUYA, Kazumoto NAGAI, Shinya SASAKAWA, Chihiro TO, Ho J Vet Med Sci Bacteriology Six atypical Actinobacillus pleuropneumoniae serovar 15 strains were isolated from pneumonic lesions of naturally infected dead pigs from the same farm in Japan. Genetic analyses of apx genes revealed that the atypical isolates contained the toxin-associated genes apxIBD, apxIIICA, apxIIIBD, and apxIVA, but not apxIICA. Coinciding with the result of the atypical gene profile, analyses of toxin protein production revealed that these atypical isolates expressed only ApxIII but not ApxII. A mouse pathogenicity test showed that the atypical isolate tested seemed to be less virulent than the typical isolates. This is the first report describing the emergence of atypical A. pleuropneumoniae serovar 15, which does not produce ApxII due to the absence of apxIICA genes, in Japan. The Japanese Society of Veterinary Science 2019-02-01 2019-03 /pmc/articles/PMC6451906/ /pubmed/30713216 http://dx.doi.org/10.1292/jvms.18-0421 Text en ©2019 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Bacteriology
TESHIMA, Kaho
HIRANO, Haruna
USHIYAMA, Kazutada
SHIBUYA, Kazumoto
NAGAI, Shinya
SASAKAWA, Chihiro
TO, Ho
Isolation and characterization of atypical Actinobacillus pleuropneumoniae serovar 15 lacking the apxIICA genes in Japan
title Isolation and characterization of atypical Actinobacillus pleuropneumoniae serovar 15 lacking the apxIICA genes in Japan
title_full Isolation and characterization of atypical Actinobacillus pleuropneumoniae serovar 15 lacking the apxIICA genes in Japan
title_fullStr Isolation and characterization of atypical Actinobacillus pleuropneumoniae serovar 15 lacking the apxIICA genes in Japan
title_full_unstemmed Isolation and characterization of atypical Actinobacillus pleuropneumoniae serovar 15 lacking the apxIICA genes in Japan
title_short Isolation and characterization of atypical Actinobacillus pleuropneumoniae serovar 15 lacking the apxIICA genes in Japan
title_sort isolation and characterization of atypical actinobacillus pleuropneumoniae serovar 15 lacking the apxiica genes in japan
topic Bacteriology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6451906/
https://www.ncbi.nlm.nih.gov/pubmed/30713216
http://dx.doi.org/10.1292/jvms.18-0421
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