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mRNA expression of tumor-associated genes in canine grade I meningiomas
This study was undertaken to establish a method for measuring mRNA expression by using real-time RT-PCR in the diagnosis of canine meningiomas. When performing real-time RT-PCR, it is essential to include appropriate control tissues and to select appropriate housekeeping genes as an internal standar...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Japanese Society of Veterinary Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6451920/ https://www.ncbi.nlm.nih.gov/pubmed/30674739 http://dx.doi.org/10.1292/jvms.18-0491 |
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author | OKABAYASHI, Ken NARITA, Takanori TAKASHIRO, Saki NADAOKA, Sawako KANAI, Shuichiro ITO, Daisuke KITAGAWA, Masato |
author_facet | OKABAYASHI, Ken NARITA, Takanori TAKASHIRO, Saki NADAOKA, Sawako KANAI, Shuichiro ITO, Daisuke KITAGAWA, Masato |
author_sort | OKABAYASHI, Ken |
collection | PubMed |
description | This study was undertaken to establish a method for measuring mRNA expression by using real-time RT-PCR in the diagnosis of canine meningiomas. When performing real-time RT-PCR, it is essential to include appropriate control tissues and to select appropriate housekeeping genes as an internal standard. Based on the results of our study, RPS18 constitutes a suitable internal standard for the comparison of mRNA expression between normal meninges and meningiomas. The results showed increased mRNA expression of VEGFA and EGFR; however, mRNA expression of KDR was reduced. Measuring mRNA expression by using real-time RT-PCR with appropriate control tissues and internal standards can provide useful information to understanding the pathogenesis of canine meningiomas, which corresponds with immunohistochemical findings. |
format | Online Article Text |
id | pubmed-6451920 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Japanese Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-64519202019-04-10 mRNA expression of tumor-associated genes in canine grade I meningiomas OKABAYASHI, Ken NARITA, Takanori TAKASHIRO, Saki NADAOKA, Sawako KANAI, Shuichiro ITO, Daisuke KITAGAWA, Masato J Vet Med Sci Surgery This study was undertaken to establish a method for measuring mRNA expression by using real-time RT-PCR in the diagnosis of canine meningiomas. When performing real-time RT-PCR, it is essential to include appropriate control tissues and to select appropriate housekeeping genes as an internal standard. Based on the results of our study, RPS18 constitutes a suitable internal standard for the comparison of mRNA expression between normal meninges and meningiomas. The results showed increased mRNA expression of VEGFA and EGFR; however, mRNA expression of KDR was reduced. Measuring mRNA expression by using real-time RT-PCR with appropriate control tissues and internal standards can provide useful information to understanding the pathogenesis of canine meningiomas, which corresponds with immunohistochemical findings. The Japanese Society of Veterinary Science 2019-01-22 2019-03 /pmc/articles/PMC6451920/ /pubmed/30674739 http://dx.doi.org/10.1292/jvms.18-0491 Text en ©2019 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Surgery OKABAYASHI, Ken NARITA, Takanori TAKASHIRO, Saki NADAOKA, Sawako KANAI, Shuichiro ITO, Daisuke KITAGAWA, Masato mRNA expression of tumor-associated genes in canine grade I meningiomas |
title | mRNA expression of tumor-associated genes in canine grade I meningiomas |
title_full | mRNA expression of tumor-associated genes in canine grade I meningiomas |
title_fullStr | mRNA expression of tumor-associated genes in canine grade I meningiomas |
title_full_unstemmed | mRNA expression of tumor-associated genes in canine grade I meningiomas |
title_short | mRNA expression of tumor-associated genes in canine grade I meningiomas |
title_sort | mrna expression of tumor-associated genes in canine grade i meningiomas |
topic | Surgery |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6451920/ https://www.ncbi.nlm.nih.gov/pubmed/30674739 http://dx.doi.org/10.1292/jvms.18-0491 |
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