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mRNA expression of tumor-associated genes in canine grade I meningiomas

This study was undertaken to establish a method for measuring mRNA expression by using real-time RT-PCR in the diagnosis of canine meningiomas. When performing real-time RT-PCR, it is essential to include appropriate control tissues and to select appropriate housekeeping genes as an internal standar...

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Autores principales: OKABAYASHI, Ken, NARITA, Takanori, TAKASHIRO, Saki, NADAOKA, Sawako, KANAI, Shuichiro, ITO, Daisuke, KITAGAWA, Masato
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6451920/
https://www.ncbi.nlm.nih.gov/pubmed/30674739
http://dx.doi.org/10.1292/jvms.18-0491
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author OKABAYASHI, Ken
NARITA, Takanori
TAKASHIRO, Saki
NADAOKA, Sawako
KANAI, Shuichiro
ITO, Daisuke
KITAGAWA, Masato
author_facet OKABAYASHI, Ken
NARITA, Takanori
TAKASHIRO, Saki
NADAOKA, Sawako
KANAI, Shuichiro
ITO, Daisuke
KITAGAWA, Masato
author_sort OKABAYASHI, Ken
collection PubMed
description This study was undertaken to establish a method for measuring mRNA expression by using real-time RT-PCR in the diagnosis of canine meningiomas. When performing real-time RT-PCR, it is essential to include appropriate control tissues and to select appropriate housekeeping genes as an internal standard. Based on the results of our study, RPS18 constitutes a suitable internal standard for the comparison of mRNA expression between normal meninges and meningiomas. The results showed increased mRNA expression of VEGFA and EGFR; however, mRNA expression of KDR was reduced. Measuring mRNA expression by using real-time RT-PCR with appropriate control tissues and internal standards can provide useful information to understanding the pathogenesis of canine meningiomas, which corresponds with immunohistochemical findings.
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spelling pubmed-64519202019-04-10 mRNA expression of tumor-associated genes in canine grade I meningiomas OKABAYASHI, Ken NARITA, Takanori TAKASHIRO, Saki NADAOKA, Sawako KANAI, Shuichiro ITO, Daisuke KITAGAWA, Masato J Vet Med Sci Surgery This study was undertaken to establish a method for measuring mRNA expression by using real-time RT-PCR in the diagnosis of canine meningiomas. When performing real-time RT-PCR, it is essential to include appropriate control tissues and to select appropriate housekeeping genes as an internal standard. Based on the results of our study, RPS18 constitutes a suitable internal standard for the comparison of mRNA expression between normal meninges and meningiomas. The results showed increased mRNA expression of VEGFA and EGFR; however, mRNA expression of KDR was reduced. Measuring mRNA expression by using real-time RT-PCR with appropriate control tissues and internal standards can provide useful information to understanding the pathogenesis of canine meningiomas, which corresponds with immunohistochemical findings. The Japanese Society of Veterinary Science 2019-01-22 2019-03 /pmc/articles/PMC6451920/ /pubmed/30674739 http://dx.doi.org/10.1292/jvms.18-0491 Text en ©2019 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Surgery
OKABAYASHI, Ken
NARITA, Takanori
TAKASHIRO, Saki
NADAOKA, Sawako
KANAI, Shuichiro
ITO, Daisuke
KITAGAWA, Masato
mRNA expression of tumor-associated genes in canine grade I meningiomas
title mRNA expression of tumor-associated genes in canine grade I meningiomas
title_full mRNA expression of tumor-associated genes in canine grade I meningiomas
title_fullStr mRNA expression of tumor-associated genes in canine grade I meningiomas
title_full_unstemmed mRNA expression of tumor-associated genes in canine grade I meningiomas
title_short mRNA expression of tumor-associated genes in canine grade I meningiomas
title_sort mrna expression of tumor-associated genes in canine grade i meningiomas
topic Surgery
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6451920/
https://www.ncbi.nlm.nih.gov/pubmed/30674739
http://dx.doi.org/10.1292/jvms.18-0491
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