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Clinicopathological significance of human leukocyte antigen F-associated transcript 10 expression in colorectal cancer
BACKGROUND: Colorectal cancer (CRC) is a common malignancy of the gastrointestinal tract. The worldwide mortality rate of CRC is about one half of its morbidity. Ubiquitin is a key regulatory factor in the cell cycle and widely exists in eukaryotes. Human leukocyte antigen F-associated transcript 10...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Baishideng Publishing Group Inc
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6451929/ https://www.ncbi.nlm.nih.gov/pubmed/30984346 http://dx.doi.org/10.4251/wjgo.v11.i1.9 |
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author | Zhang, Chun-Yang Sun, Jie Wang, Xing Wang, Cui-Fang Zeng, Xian-Dong |
author_facet | Zhang, Chun-Yang Sun, Jie Wang, Xing Wang, Cui-Fang Zeng, Xian-Dong |
author_sort | Zhang, Chun-Yang |
collection | PubMed |
description | BACKGROUND: Colorectal cancer (CRC) is a common malignancy of the gastrointestinal tract. The worldwide mortality rate of CRC is about one half of its morbidity. Ubiquitin is a key regulatory factor in the cell cycle and widely exists in eukaryotes. Human leukocyte antigen F-associated transcript 10 (FAT10), known as diubiquitin, is an 18 kDa protein with 29% and 36% homology with the N and C termini of ubiquitin. The function of FAT10 has not been fully elucidated, and some studies have shown that it plays an important role in various cell processes. AIM: To examine FAT10 expression and to analyze the relationship between FAT10 expression and the clinicopathological parameters of CRC. METHODS: FAT10 expression in 61 cases of CRC and para-cancer colorectal tissues was measured by immunohistochemistry and Western blotting. The relationship between FAT10 expression and clinicopathological parameters of CRC was statistically analyzed. RESULTS: Immunohistochemical analysis showed that the positive rate of FAT10 expression in CRC (63.93%) was significantly higher than that in tumor-adjacent tissues (9.84%, P < 0.05) and normal colorectal mucosal tissue (1.64%, P < 0.05). Western blotting also indicated that FAT10 expression was significantly higher in CRC than in tumor-adjacent tissue (P < 0.05). FAT10 expression was closely associated with clinical stage and lymphatic spread of CRC. FAT10 expression also positively correlated with p53 expression. CONCLUSION: FAT10 expression is highly upregulated in CRC. FAT10 expression is closely associated with clinical stage and lymphatic spread of CRC. |
format | Online Article Text |
id | pubmed-6451929 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Baishideng Publishing Group Inc |
record_format | MEDLINE/PubMed |
spelling | pubmed-64519292019-04-12 Clinicopathological significance of human leukocyte antigen F-associated transcript 10 expression in colorectal cancer Zhang, Chun-Yang Sun, Jie Wang, Xing Wang, Cui-Fang Zeng, Xian-Dong World J Gastrointest Oncol Basic Study BACKGROUND: Colorectal cancer (CRC) is a common malignancy of the gastrointestinal tract. The worldwide mortality rate of CRC is about one half of its morbidity. Ubiquitin is a key regulatory factor in the cell cycle and widely exists in eukaryotes. Human leukocyte antigen F-associated transcript 10 (FAT10), known as diubiquitin, is an 18 kDa protein with 29% and 36% homology with the N and C termini of ubiquitin. The function of FAT10 has not been fully elucidated, and some studies have shown that it plays an important role in various cell processes. AIM: To examine FAT10 expression and to analyze the relationship between FAT10 expression and the clinicopathological parameters of CRC. METHODS: FAT10 expression in 61 cases of CRC and para-cancer colorectal tissues was measured by immunohistochemistry and Western blotting. The relationship between FAT10 expression and clinicopathological parameters of CRC was statistically analyzed. RESULTS: Immunohistochemical analysis showed that the positive rate of FAT10 expression in CRC (63.93%) was significantly higher than that in tumor-adjacent tissues (9.84%, P < 0.05) and normal colorectal mucosal tissue (1.64%, P < 0.05). Western blotting also indicated that FAT10 expression was significantly higher in CRC than in tumor-adjacent tissue (P < 0.05). FAT10 expression was closely associated with clinical stage and lymphatic spread of CRC. FAT10 expression also positively correlated with p53 expression. CONCLUSION: FAT10 expression is highly upregulated in CRC. FAT10 expression is closely associated with clinical stage and lymphatic spread of CRC. Baishideng Publishing Group Inc 2019-01-15 2019-01-15 /pmc/articles/PMC6451929/ /pubmed/30984346 http://dx.doi.org/10.4251/wjgo.v11.i1.9 Text en ©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved. http://creativecommons.org/licenses/by-nc/4.0/ This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. |
spellingShingle | Basic Study Zhang, Chun-Yang Sun, Jie Wang, Xing Wang, Cui-Fang Zeng, Xian-Dong Clinicopathological significance of human leukocyte antigen F-associated transcript 10 expression in colorectal cancer |
title | Clinicopathological significance of human leukocyte antigen F-associated transcript 10 expression in colorectal cancer |
title_full | Clinicopathological significance of human leukocyte antigen F-associated transcript 10 expression in colorectal cancer |
title_fullStr | Clinicopathological significance of human leukocyte antigen F-associated transcript 10 expression in colorectal cancer |
title_full_unstemmed | Clinicopathological significance of human leukocyte antigen F-associated transcript 10 expression in colorectal cancer |
title_short | Clinicopathological significance of human leukocyte antigen F-associated transcript 10 expression in colorectal cancer |
title_sort | clinicopathological significance of human leukocyte antigen f-associated transcript 10 expression in colorectal cancer |
topic | Basic Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6451929/ https://www.ncbi.nlm.nih.gov/pubmed/30984346 http://dx.doi.org/10.4251/wjgo.v11.i1.9 |
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