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Optimizing the production and affinity purification of HIV-1 envelope glycoprotein SOSIP trimers from transiently transfected CHO cells

We describe methods to improve the efficiency with which HIV-1 Envelope glycoprotein SOSIP trimer immunogens can be produced by transient transfection of ExpiCHO-S cells and then affinity purified using the trimer-specific human monoclonal antibody PGT145. The specificity of PGT145 for properly fold...

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Detalles Bibliográficos
Autores principales:	Cupo, Albert, Cruz Portillo, Victor M., Gelfand, Paul, Yasmeen, Anila, Klasse, P. J., Moore, John P.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2019
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6453562/ https://www.ncbi.nlm.nih.gov/pubmed/30958859 http://dx.doi.org/10.1371/journal.pone.0215106

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