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Importin-9 wraps around the H2A-H2B core to act as nuclear importer and histone chaperone
We report the crystal structure of nuclear import receptor Importin-9 bound to its cargo, the histones H2A-H2B. Importin-9 wraps around the core, globular region of H2A-H2B to form an extensive interface. The nature of this interface coupled with quantitative analysis of deletion mutants of H2A-H2B...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6453568/ https://www.ncbi.nlm.nih.gov/pubmed/30855230 http://dx.doi.org/10.7554/eLife.43630 |
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author | Padavannil, Abhilash Sarkar, Prithwijit Kim, Seung Joong Cagatay, Tolga Jiou, Jenny Brautigam, Chad A Tomchick, Diana R Sali, Andrej D'Arcy, Sheena Chook, Yuh Min |
author_facet | Padavannil, Abhilash Sarkar, Prithwijit Kim, Seung Joong Cagatay, Tolga Jiou, Jenny Brautigam, Chad A Tomchick, Diana R Sali, Andrej D'Arcy, Sheena Chook, Yuh Min |
author_sort | Padavannil, Abhilash |
collection | PubMed |
description | We report the crystal structure of nuclear import receptor Importin-9 bound to its cargo, the histones H2A-H2B. Importin-9 wraps around the core, globular region of H2A-H2B to form an extensive interface. The nature of this interface coupled with quantitative analysis of deletion mutants of H2A-H2B suggests that the NLS-like sequences in the H2A-H2B tails play a minor role in import. Importin-9•H2A-H2B is reminiscent of interactions between histones and histone chaperones in that it precludes H2A-H2B interactions with DNA and H3-H4 as seen in the nucleosome. Like many histone chaperones, which prevent inappropriate non-nucleosomal interactions, Importin-9 also sequesters H2A-H2B from DNA. Importin-9 appears to act as a storage chaperone for H2A-H2B while escorting it to the nucleus. Surprisingly, RanGTP does not dissociate Importin-9•H2A-H2B but assembles into a RanGTP•Importin-9•H2A-H2B complex. The presence of Ran in the complex, however, modulates Imp9-H2A-H2B interactions to facilitate its dissociation by DNA and assembly into a nucleosome. |
format | Online Article Text |
id | pubmed-6453568 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-64535682019-04-10 Importin-9 wraps around the H2A-H2B core to act as nuclear importer and histone chaperone Padavannil, Abhilash Sarkar, Prithwijit Kim, Seung Joong Cagatay, Tolga Jiou, Jenny Brautigam, Chad A Tomchick, Diana R Sali, Andrej D'Arcy, Sheena Chook, Yuh Min eLife Structural Biology and Molecular Biophysics We report the crystal structure of nuclear import receptor Importin-9 bound to its cargo, the histones H2A-H2B. Importin-9 wraps around the core, globular region of H2A-H2B to form an extensive interface. The nature of this interface coupled with quantitative analysis of deletion mutants of H2A-H2B suggests that the NLS-like sequences in the H2A-H2B tails play a minor role in import. Importin-9•H2A-H2B is reminiscent of interactions between histones and histone chaperones in that it precludes H2A-H2B interactions with DNA and H3-H4 as seen in the nucleosome. Like many histone chaperones, which prevent inappropriate non-nucleosomal interactions, Importin-9 also sequesters H2A-H2B from DNA. Importin-9 appears to act as a storage chaperone for H2A-H2B while escorting it to the nucleus. Surprisingly, RanGTP does not dissociate Importin-9•H2A-H2B but assembles into a RanGTP•Importin-9•H2A-H2B complex. The presence of Ran in the complex, however, modulates Imp9-H2A-H2B interactions to facilitate its dissociation by DNA and assembly into a nucleosome. eLife Sciences Publications, Ltd 2019-03-11 /pmc/articles/PMC6453568/ /pubmed/30855230 http://dx.doi.org/10.7554/eLife.43630 Text en © 2019, Padavannil et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Structural Biology and Molecular Biophysics Padavannil, Abhilash Sarkar, Prithwijit Kim, Seung Joong Cagatay, Tolga Jiou, Jenny Brautigam, Chad A Tomchick, Diana R Sali, Andrej D'Arcy, Sheena Chook, Yuh Min Importin-9 wraps around the H2A-H2B core to act as nuclear importer and histone chaperone |
title | Importin-9 wraps around the H2A-H2B core to act as nuclear importer and histone chaperone |
title_full | Importin-9 wraps around the H2A-H2B core to act as nuclear importer and histone chaperone |
title_fullStr | Importin-9 wraps around the H2A-H2B core to act as nuclear importer and histone chaperone |
title_full_unstemmed | Importin-9 wraps around the H2A-H2B core to act as nuclear importer and histone chaperone |
title_short | Importin-9 wraps around the H2A-H2B core to act as nuclear importer and histone chaperone |
title_sort | importin-9 wraps around the h2a-h2b core to act as nuclear importer and histone chaperone |
topic | Structural Biology and Molecular Biophysics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6453568/ https://www.ncbi.nlm.nih.gov/pubmed/30855230 http://dx.doi.org/10.7554/eLife.43630 |
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