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Nondestructive, base-resolution sequencing of 5-hydroxymethylcytosine using a DNA deaminase

Here we present APOBEC-Coupled Epigenetic Sequencing (ACE-Seq), a bisulfite-free method for localizing 5-hydroxymethylcytosine (5hmC) at single-base resolution with low DNA input. The method builds upon the observation that AID/APOBEC family DNA deaminase enzymes can potently discriminate between cy...

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Detalles Bibliográficos
Autores principales:	Schutsky, Emily K., DeNizio, Jamie E., Hu, Peng, Liu, Monica Yun, Nabel, Christopher S., Fabyanic, Emily B., Hwang, Young, Bushman, Frederic D., Wu, Hao, Kohli, Rahul M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6453757/ https://www.ncbi.nlm.nih.gov/pubmed/30295673 http://dx.doi.org/10.1038/nbt.4204

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