Cargando…
Cascade Signals of Papaverine Inhibiting LPS-Induced Retinal Microglial Activation
Studies have shown that papaverine can inhibit lipopolysaccharide (LPS)-induced microglial activation. The retinal primary microglia of newborn SD rats were isolated and purified, and a LPS-induced microglia activation model was established. The protein phosphorylation level of the signaling pathway...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6453874/ https://www.ncbi.nlm.nih.gov/pubmed/30852743 http://dx.doi.org/10.1007/s12031-019-01289-w |
_version_ | 1783409454026326016 |
---|---|
author | Zhou, Ting Zhu, Yu |
author_facet | Zhou, Ting Zhu, Yu |
author_sort | Zhou, Ting |
collection | PubMed |
description | Studies have shown that papaverine can inhibit lipopolysaccharide (LPS)-induced microglial activation. The retinal primary microglia of newborn SD rats were isolated and purified, and a LPS-induced microglia activation model was established. The protein phosphorylation level of the signaling pathway was detected by western blotting. The transcription and expression of TNF-α, IL-1β, and IL-10 were respectively detected by RT-PCR and ELISA to observe the abnormal activation of primary microglia. The cAMP inhibitor Rp-isomer, PKA inhibitor H89, and MEK inhibitor U0126 were separately added to further investigate the role of MEK/Erk in PAP inhibition of primary microglial activation and the relationship between cAMP/PKA and MEK/Erk. It was found that the level of MEK phosphorylation was upregulated after LPS stimulation, which was blocked by 10 μg/ml of papaverine.10μM U0126 significantly inhibited TNF-α and IL-1β and increased IL-10 transcription and expression in retinal microglia (P < 0.01). Both Rp-isomer and H89 upregulated the phosphorylation levels of MEK and Erk. Papaverine may inhibit inflammatory factors and promote the expression of anti-inflammatory factors through the cAMP/PKA and MEK/Erk pathway, thereby inhibiting LPS-induced activation of primary retinal microglia, and the MEK/Erk pathway may be partially regulated by cAMP/PKA, which can provide theoretical basis and experimental basis for its protection of the central nervous system. |
format | Online Article Text |
id | pubmed-6453874 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-64538742019-04-26 Cascade Signals of Papaverine Inhibiting LPS-Induced Retinal Microglial Activation Zhou, Ting Zhu, Yu J Mol Neurosci Article Studies have shown that papaverine can inhibit lipopolysaccharide (LPS)-induced microglial activation. The retinal primary microglia of newborn SD rats were isolated and purified, and a LPS-induced microglia activation model was established. The protein phosphorylation level of the signaling pathway was detected by western blotting. The transcription and expression of TNF-α, IL-1β, and IL-10 were respectively detected by RT-PCR and ELISA to observe the abnormal activation of primary microglia. The cAMP inhibitor Rp-isomer, PKA inhibitor H89, and MEK inhibitor U0126 were separately added to further investigate the role of MEK/Erk in PAP inhibition of primary microglial activation and the relationship between cAMP/PKA and MEK/Erk. It was found that the level of MEK phosphorylation was upregulated after LPS stimulation, which was blocked by 10 μg/ml of papaverine.10μM U0126 significantly inhibited TNF-α and IL-1β and increased IL-10 transcription and expression in retinal microglia (P < 0.01). Both Rp-isomer and H89 upregulated the phosphorylation levels of MEK and Erk. Papaverine may inhibit inflammatory factors and promote the expression of anti-inflammatory factors through the cAMP/PKA and MEK/Erk pathway, thereby inhibiting LPS-induced activation of primary retinal microglia, and the MEK/Erk pathway may be partially regulated by cAMP/PKA, which can provide theoretical basis and experimental basis for its protection of the central nervous system. Springer US 2019-03-09 2019 /pmc/articles/PMC6453874/ /pubmed/30852743 http://dx.doi.org/10.1007/s12031-019-01289-w Text en © The Author(s) 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Article Zhou, Ting Zhu, Yu Cascade Signals of Papaverine Inhibiting LPS-Induced Retinal Microglial Activation |
title | Cascade Signals of Papaverine Inhibiting LPS-Induced Retinal Microglial Activation |
title_full | Cascade Signals of Papaverine Inhibiting LPS-Induced Retinal Microglial Activation |
title_fullStr | Cascade Signals of Papaverine Inhibiting LPS-Induced Retinal Microglial Activation |
title_full_unstemmed | Cascade Signals of Papaverine Inhibiting LPS-Induced Retinal Microglial Activation |
title_short | Cascade Signals of Papaverine Inhibiting LPS-Induced Retinal Microglial Activation |
title_sort | cascade signals of papaverine inhibiting lps-induced retinal microglial activation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6453874/ https://www.ncbi.nlm.nih.gov/pubmed/30852743 http://dx.doi.org/10.1007/s12031-019-01289-w |
work_keys_str_mv | AT zhouting cascadesignalsofpapaverineinhibitinglpsinducedretinalmicroglialactivation AT zhuyu cascadesignalsofpapaverineinhibitinglpsinducedretinalmicroglialactivation |