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Adaptations to high-intensity interval training in skeletal muscle require NADPH oxidase 2

OBJECTIVE: Reactive oxygen species (ROS) have been proposed as signaling molecules mediating exercise training adaptation, but the ROS source has remained unclear. This study aimed to investigate if increased NADPH oxidase (NOX)2-dependent activity during exercise is required for long-term high-inte...

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Autores principales: Henríquez-Olguín, Carlos, Renani, Leila Baghersad, Arab-Ceschia, Lyne, Raun, Steffen H., Bhatia, Aakash, Li, Zhencheng, Knudsen, Jonas R., Holmdahl, Rikard, Jensen, Thomas E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6454063/
https://www.ncbi.nlm.nih.gov/pubmed/30959461
http://dx.doi.org/10.1016/j.redox.2019.101188
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author Henríquez-Olguín, Carlos
Renani, Leila Baghersad
Arab-Ceschia, Lyne
Raun, Steffen H.
Bhatia, Aakash
Li, Zhencheng
Knudsen, Jonas R.
Holmdahl, Rikard
Jensen, Thomas E.
author_facet Henríquez-Olguín, Carlos
Renani, Leila Baghersad
Arab-Ceschia, Lyne
Raun, Steffen H.
Bhatia, Aakash
Li, Zhencheng
Knudsen, Jonas R.
Holmdahl, Rikard
Jensen, Thomas E.
author_sort Henríquez-Olguín, Carlos
collection PubMed
description OBJECTIVE: Reactive oxygen species (ROS) have been proposed as signaling molecules mediating exercise training adaptation, but the ROS source has remained unclear. This study aimed to investigate if increased NADPH oxidase (NOX)2-dependent activity during exercise is required for long-term high-intensity interval training (HIIT) in skeletal muscle using a mouse model lacking functional NOX2 complex due to absent p47phox (Ncf1) subunit expression (ncf1* mutation). METHODS: HIIT was investigated after an acute bout of exercise and after a chronic intervention (3x/week for 6 weeks) in wild-type (WT) vs. NOX2 activity-deficient (ncf1*) mice. NOX2 activation during HIIT was measured using an electroporated genetically-encoded biosensor. Immunoblotting and single-fiber microscopy was performed to measure classical exercise-training responsive endpoints in skeletal muscle. RESULTS: A single bout of HIIT increased NOX2 activity measured as p47-roGFP oxidation immediately after exercise but not 1 h or 4 h after exercise. After a 6-week HIIT regimen, improvements in maximal running capacity and some muscle training-markers responded less to HIIT in the ncf1* mice compared to WT, including superoxide dismutase 2, catalase, hexokinase II, pyruvate dehydrogenase and protein markers of mitochondrial oxidative phosphorylation complexes. Strikingly, HIIT-training increased mitochondrial network area and decreased fragmentation in WT mice only. CONCLUSION: This study suggests that HIIT exercise increases NOX2 activity in skeletal muscle and shows that NOX2 activity is required for specific skeletal muscle adaptations to HIIT relating to antioxidant defense, glucose metabolism, and mitochondria.
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spelling pubmed-64540632019-04-19 Adaptations to high-intensity interval training in skeletal muscle require NADPH oxidase 2 Henríquez-Olguín, Carlos Renani, Leila Baghersad Arab-Ceschia, Lyne Raun, Steffen H. Bhatia, Aakash Li, Zhencheng Knudsen, Jonas R. Holmdahl, Rikard Jensen, Thomas E. Redox Biol Research Paper OBJECTIVE: Reactive oxygen species (ROS) have been proposed as signaling molecules mediating exercise training adaptation, but the ROS source has remained unclear. This study aimed to investigate if increased NADPH oxidase (NOX)2-dependent activity during exercise is required for long-term high-intensity interval training (HIIT) in skeletal muscle using a mouse model lacking functional NOX2 complex due to absent p47phox (Ncf1) subunit expression (ncf1* mutation). METHODS: HIIT was investigated after an acute bout of exercise and after a chronic intervention (3x/week for 6 weeks) in wild-type (WT) vs. NOX2 activity-deficient (ncf1*) mice. NOX2 activation during HIIT was measured using an electroporated genetically-encoded biosensor. Immunoblotting and single-fiber microscopy was performed to measure classical exercise-training responsive endpoints in skeletal muscle. RESULTS: A single bout of HIIT increased NOX2 activity measured as p47-roGFP oxidation immediately after exercise but not 1 h or 4 h after exercise. After a 6-week HIIT regimen, improvements in maximal running capacity and some muscle training-markers responded less to HIIT in the ncf1* mice compared to WT, including superoxide dismutase 2, catalase, hexokinase II, pyruvate dehydrogenase and protein markers of mitochondrial oxidative phosphorylation complexes. Strikingly, HIIT-training increased mitochondrial network area and decreased fragmentation in WT mice only. CONCLUSION: This study suggests that HIIT exercise increases NOX2 activity in skeletal muscle and shows that NOX2 activity is required for specific skeletal muscle adaptations to HIIT relating to antioxidant defense, glucose metabolism, and mitochondria. Elsevier 2019-04-03 /pmc/articles/PMC6454063/ /pubmed/30959461 http://dx.doi.org/10.1016/j.redox.2019.101188 Text en © 2019 Published by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Paper
Henríquez-Olguín, Carlos
Renani, Leila Baghersad
Arab-Ceschia, Lyne
Raun, Steffen H.
Bhatia, Aakash
Li, Zhencheng
Knudsen, Jonas R.
Holmdahl, Rikard
Jensen, Thomas E.
Adaptations to high-intensity interval training in skeletal muscle require NADPH oxidase 2
title Adaptations to high-intensity interval training in skeletal muscle require NADPH oxidase 2
title_full Adaptations to high-intensity interval training in skeletal muscle require NADPH oxidase 2
title_fullStr Adaptations to high-intensity interval training in skeletal muscle require NADPH oxidase 2
title_full_unstemmed Adaptations to high-intensity interval training in skeletal muscle require NADPH oxidase 2
title_short Adaptations to high-intensity interval training in skeletal muscle require NADPH oxidase 2
title_sort adaptations to high-intensity interval training in skeletal muscle require nadph oxidase 2
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6454063/
https://www.ncbi.nlm.nih.gov/pubmed/30959461
http://dx.doi.org/10.1016/j.redox.2019.101188
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