Determination of Highest Dose of Ammonia without Effect at Work Environment through the Expression of Interleukin-2 Cell in Rattus Novergicus

BACKGROUND: For determining the threshold limit value firstly determined the highest dose of ammonia without effect (NOAEL). However, research on the determination of NOAEL ammonia didn’t conduct in Indonesia. AIM: The aim of this study to determine the value of the highest dose of ammonia without effect (No Observed Adverse Effect Level/NOAEL) through interleukin-2 (IL-2) expression on white mice. METHODS: This study used experimental laboratory research with post-test only control group design using white mice as experimental subjects. The treatment group divided into 6 groups (a group of controls and five groups with different ammonia exposure through inhalation). The trend curve of Remmele Scale Index (IRS) and histopathologic analysis could be used for determining NOAEL. RESULT: The location of the highest dose of ammonia without any effect (NOAEL) of white mice was in the second group, with 0.0103 mg/kg body weight dose. Analysis of statistical tests Kruskal Wallis stated there was no significant difference in interleukin-2 expression between the control with ammonia exposed group with a significance of p (0.747) > α (0.05). CONCLUSION: There is no difference between some interleukin-2 expression in the lymphocyte cell lung white mice group exposed to ammonia and control group. The highest dose of ammonia without any effect (NOAEL) on white mice was 0.0103 mg/kg body weight.