Tunable structured illumination light sheet microscopy for background rejection and imaging depth in minimally processed tissues

We demonstrate improved optical sectioning in light sheet fluorescence microscopy using tunable structured illumination (SI) frequencies to optimize image quality in scattering specimens. The SI patterns are generated coherently using a one-dimensional spatial light modulator for maximum pattern con...

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Detalles Bibliográficos
Autores principales: Landry, Joseph R., Itoh, Ryosuke, Li, Jonathan M., Hamann, Stephen S., Mandella, Michael, Contag, Christopher H., Solgaard, Olav
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society of Photo-Optical Instrumentation Engineers 2019
Materias:
Microscopy
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6454294/
https://www.ncbi.nlm.nih.gov/pubmed/30968649
http://dx.doi.org/10.1117/1.JBO.24.4.046501
Descripción
Sumario:We demonstrate improved optical sectioning in light sheet fluorescence microscopy using tunable structured illumination (SI) frequencies to optimize image quality in scattering specimens. The SI patterns are generated coherently using a one-dimensional spatial light modulator for maximum pattern contrast, and the pattern spatial frequency is adjustable up to half the incoherent cutoff frequency of our detection objective. At this frequency, we demonstrate background reductions of 2 orders of magnitude.

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