Tunable structured illumination light sheet microscopy for background rejection and imaging depth in minimally processed tissues

We demonstrate improved optical sectioning in light sheet fluorescence microscopy using tunable structured illumination (SI) frequencies to optimize image quality in scattering specimens. The SI patterns are generated coherently using a one-dimensional spatial light modulator for maximum pattern con...

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Detalles Bibliográficos
Autores principales: Landry, Joseph R., Itoh, Ryosuke, Li, Jonathan M., Hamann, Stephen S., Mandella, Michael, Contag, Christopher H., Solgaard, Olav
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society of Photo-Optical Instrumentation Engineers 2019
Materias:
Microscopy
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6454294/
https://www.ncbi.nlm.nih.gov/pubmed/30968649
http://dx.doi.org/10.1117/1.JBO.24.4.046501
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author Landry, Joseph R.
Itoh, Ryosuke
Li, Jonathan M.
Hamann, Stephen S.
Mandella, Michael
Contag, Christopher H.
Solgaard, Olav
author_facet Landry, Joseph R.
Itoh, Ryosuke
Li, Jonathan M.
Hamann, Stephen S.
Mandella, Michael
Contag, Christopher H.
Solgaard, Olav
author_sort Landry, Joseph R.
collection PubMed
description We demonstrate improved optical sectioning in light sheet fluorescence microscopy using tunable structured illumination (SI) frequencies to optimize image quality in scattering specimens. The SI patterns are generated coherently using a one-dimensional spatial light modulator for maximum pattern contrast, and the pattern spatial frequency is adjustable up to half the incoherent cutoff frequency of our detection objective. At this frequency, we demonstrate background reductions of 2 orders of magnitude.
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spelling pubmed-64542942020-01-30 Tunable structured illumination light sheet microscopy for background rejection and imaging depth in minimally processed tissues Landry, Joseph R. Itoh, Ryosuke Li, Jonathan M. Hamann, Stephen S. Mandella, Michael Contag, Christopher H. Solgaard, Olav J Biomed Opt Microscopy We demonstrate improved optical sectioning in light sheet fluorescence microscopy using tunable structured illumination (SI) frequencies to optimize image quality in scattering specimens. The SI patterns are generated coherently using a one-dimensional spatial light modulator for maximum pattern contrast, and the pattern spatial frequency is adjustable up to half the incoherent cutoff frequency of our detection objective. At this frequency, we demonstrate background reductions of 2 orders of magnitude. Society of Photo-Optical Instrumentation Engineers 2019-04-09 2019-04 /pmc/articles/PMC6454294/ /pubmed/30968649 http://dx.doi.org/10.1117/1.JBO.24.4.046501 Text en © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
spellingShingle Microscopy
Landry, Joseph R.
Itoh, Ryosuke
Li, Jonathan M.
Hamann, Stephen S.
Mandella, Michael
Contag, Christopher H.
Solgaard, Olav
Tunable structured illumination light sheet microscopy for background rejection and imaging depth in minimally processed tissues
title Tunable structured illumination light sheet microscopy for background rejection and imaging depth in minimally processed tissues
title_full Tunable structured illumination light sheet microscopy for background rejection and imaging depth in minimally processed tissues
title_fullStr Tunable structured illumination light sheet microscopy for background rejection and imaging depth in minimally processed tissues
title_full_unstemmed Tunable structured illumination light sheet microscopy for background rejection and imaging depth in minimally processed tissues
title_short Tunable structured illumination light sheet microscopy for background rejection and imaging depth in minimally processed tissues
title_sort tunable structured illumination light sheet microscopy for background rejection and imaging depth in minimally processed tissues
topic Microscopy
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6454294/
https://www.ncbi.nlm.nih.gov/pubmed/30968649
http://dx.doi.org/10.1117/1.JBO.24.4.046501
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