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A 16S rDNA PCR-based theoretical to actual delta approach on culturable mock communities revealed severe losses of diversity information
BACKGROUND: Subunits of ribosomal RNA genes (rDNAs) characterized by PCR-based protocols have been the proxy for studies in microbial taxonomy, phylogenetics, evolution and ecology. However, relevant factors have shown to interfere in the experimental outputs in a variety of systems. In this work, a...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6454784/ https://www.ncbi.nlm.nih.gov/pubmed/30961521 http://dx.doi.org/10.1186/s12866-019-1446-2 |