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Diversity of phenotypically non-dermatophyte, non-Aspergillus filamentous fungi causing nail infections: importance of accurate identification and antifungal susceptibility testing

Onychomycosis is most commonly caused by dermatophytes. In this study, we examined the spectrum of phenotypically non-dermatophyte and non-Aspergillus fungal isolates recovered over a 10-year period from nails of patients with onychomycosis in Hong Kong. A total of 24 non-duplicated isolates recover...

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Autores principales: Tsang, Chi-Ching, Tang, James Y. M., Chan, Ka-Fai, Lee, Chun-Yi, Chan, Jasper F. W., Ngan, Antonio H. Y., Cheung, Mei, Lau, Eunice C. L., Li, Xin, Ng, Ricky H. Y., Lai, Christopher K. C., Fung, Kitty S. C., Lau, Susanna K. P., Woo, Patrick C. Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6455232/
https://www.ncbi.nlm.nih.gov/pubmed/30938262
http://dx.doi.org/10.1080/22221751.2019.1598781
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author Tsang, Chi-Ching
Tang, James Y. M.
Chan, Ka-Fai
Lee, Chun-Yi
Chan, Jasper F. W.
Ngan, Antonio H. Y.
Cheung, Mei
Lau, Eunice C. L.
Li, Xin
Ng, Ricky H. Y.
Lai, Christopher K. C.
Fung, Kitty S. C.
Lau, Susanna K. P.
Woo, Patrick C. Y.
author_facet Tsang, Chi-Ching
Tang, James Y. M.
Chan, Ka-Fai
Lee, Chun-Yi
Chan, Jasper F. W.
Ngan, Antonio H. Y.
Cheung, Mei
Lau, Eunice C. L.
Li, Xin
Ng, Ricky H. Y.
Lai, Christopher K. C.
Fung, Kitty S. C.
Lau, Susanna K. P.
Woo, Patrick C. Y.
author_sort Tsang, Chi-Ching
collection PubMed
description Onychomycosis is most commonly caused by dermatophytes. In this study, we examined the spectrum of phenotypically non-dermatophyte and non-Aspergillus fungal isolates recovered over a 10-year period from nails of patients with onychomycosis in Hong Kong. A total of 24 non-duplicated isolates recovered from 24 patients were included. The median age of the patients was 51 years, and two-thirds of them were males. One-third and two-thirds had finger and toe nail infections respectively. Among these 24 nail isolates, 17 were confidently identified as 13 different known fungal species, using a polyphasic approach. These 13 species belonged to 11 genera and ≥9 families. For the remaining seven isolates, multilocus sequencing did not reveal their definite species identities. These seven potentially novel species belonged to four different known and three potentially novel genera of seven families. 33.3%, 41.7% and 95.8% of the 24 fungal isolates possessed minimum inhibitory concentrations of >1 µg/mL to terbinafine, itraconazole and fluconazole, respectively, the first line treatment of onychomycosis. A high diversity of moulds was associated with onychomycosis. A significant proportion of the isolates were potentially novel fungal species. To guide proper treatment, molecular identification and antifungal susceptibility testing should be performed for these uncommonly isolated fungal species.
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spelling pubmed-64552322019-04-18 Diversity of phenotypically non-dermatophyte, non-Aspergillus filamentous fungi causing nail infections: importance of accurate identification and antifungal susceptibility testing Tsang, Chi-Ching Tang, James Y. M. Chan, Ka-Fai Lee, Chun-Yi Chan, Jasper F. W. Ngan, Antonio H. Y. Cheung, Mei Lau, Eunice C. L. Li, Xin Ng, Ricky H. Y. Lai, Christopher K. C. Fung, Kitty S. C. Lau, Susanna K. P. Woo, Patrick C. Y. Emerg Microbes Infect Article Onychomycosis is most commonly caused by dermatophytes. In this study, we examined the spectrum of phenotypically non-dermatophyte and non-Aspergillus fungal isolates recovered over a 10-year period from nails of patients with onychomycosis in Hong Kong. A total of 24 non-duplicated isolates recovered from 24 patients were included. The median age of the patients was 51 years, and two-thirds of them were males. One-third and two-thirds had finger and toe nail infections respectively. Among these 24 nail isolates, 17 were confidently identified as 13 different known fungal species, using a polyphasic approach. These 13 species belonged to 11 genera and ≥9 families. For the remaining seven isolates, multilocus sequencing did not reveal their definite species identities. These seven potentially novel species belonged to four different known and three potentially novel genera of seven families. 33.3%, 41.7% and 95.8% of the 24 fungal isolates possessed minimum inhibitory concentrations of >1 µg/mL to terbinafine, itraconazole and fluconazole, respectively, the first line treatment of onychomycosis. A high diversity of moulds was associated with onychomycosis. A significant proportion of the isolates were potentially novel fungal species. To guide proper treatment, molecular identification and antifungal susceptibility testing should be performed for these uncommonly isolated fungal species. Taylor & Francis 2019-04-02 /pmc/articles/PMC6455232/ /pubmed/30938262 http://dx.doi.org/10.1080/22221751.2019.1598781 Text en © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Tsang, Chi-Ching
Tang, James Y. M.
Chan, Ka-Fai
Lee, Chun-Yi
Chan, Jasper F. W.
Ngan, Antonio H. Y.
Cheung, Mei
Lau, Eunice C. L.
Li, Xin
Ng, Ricky H. Y.
Lai, Christopher K. C.
Fung, Kitty S. C.
Lau, Susanna K. P.
Woo, Patrick C. Y.
Diversity of phenotypically non-dermatophyte, non-Aspergillus filamentous fungi causing nail infections: importance of accurate identification and antifungal susceptibility testing
title Diversity of phenotypically non-dermatophyte, non-Aspergillus filamentous fungi causing nail infections: importance of accurate identification and antifungal susceptibility testing
title_full Diversity of phenotypically non-dermatophyte, non-Aspergillus filamentous fungi causing nail infections: importance of accurate identification and antifungal susceptibility testing
title_fullStr Diversity of phenotypically non-dermatophyte, non-Aspergillus filamentous fungi causing nail infections: importance of accurate identification and antifungal susceptibility testing
title_full_unstemmed Diversity of phenotypically non-dermatophyte, non-Aspergillus filamentous fungi causing nail infections: importance of accurate identification and antifungal susceptibility testing
title_short Diversity of phenotypically non-dermatophyte, non-Aspergillus filamentous fungi causing nail infections: importance of accurate identification and antifungal susceptibility testing
title_sort diversity of phenotypically non-dermatophyte, non-aspergillus filamentous fungi causing nail infections: importance of accurate identification and antifungal susceptibility testing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6455232/
https://www.ncbi.nlm.nih.gov/pubmed/30938262
http://dx.doi.org/10.1080/22221751.2019.1598781
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