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The p38/HOG stress-activated protein kinase network couples growth to division in Candida albicans
Cell size is a complex trait that responds to developmental and environmental cues. Quantitative size analysis of mutant strain collections disrupted for protein kinases and transcriptional regulators in the pathogenic yeast Candida albicans uncovered 66 genes that altered cell size, few of which ov...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456229/ https://www.ncbi.nlm.nih.gov/pubmed/30921326 http://dx.doi.org/10.1371/journal.pgen.1008052 |
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author | Sellam, Adnane Chaillot, Julien Mallick, Jaideep Tebbji, Faiza Richard Albert, Julien Cook, Michael A. Tyers, Mike |
author_facet | Sellam, Adnane Chaillot, Julien Mallick, Jaideep Tebbji, Faiza Richard Albert, Julien Cook, Michael A. Tyers, Mike |
author_sort | Sellam, Adnane |
collection | PubMed |
description | Cell size is a complex trait that responds to developmental and environmental cues. Quantitative size analysis of mutant strain collections disrupted for protein kinases and transcriptional regulators in the pathogenic yeast Candida albicans uncovered 66 genes that altered cell size, few of which overlapped with known size genes in the budding yeast Saccharomyces cerevisiae. A potent size regulator specific to C. albicans was the conserved p38/HOG MAPK module that mediates the osmostress response. Basal HOG activity inhibited the SBF G1/S transcription factor complex in a stress-independent fashion to delay the G1/S transition. The HOG network also governed ribosome biogenesis through the master transcriptional regulator Sfp1. Hog1 bound to the promoters and cognate transcription factors for ribosome biogenesis regulons and interacted genetically with the SBF G1/S machinery, and thereby directly linked cell growth and division. These results illuminate the evolutionary plasticity of size control and identify the HOG module as a nexus of cell cycle and growth regulation. |
format | Online Article Text |
id | pubmed-6456229 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-64562292019-05-03 The p38/HOG stress-activated protein kinase network couples growth to division in Candida albicans Sellam, Adnane Chaillot, Julien Mallick, Jaideep Tebbji, Faiza Richard Albert, Julien Cook, Michael A. Tyers, Mike PLoS Genet Research Article Cell size is a complex trait that responds to developmental and environmental cues. Quantitative size analysis of mutant strain collections disrupted for protein kinases and transcriptional regulators in the pathogenic yeast Candida albicans uncovered 66 genes that altered cell size, few of which overlapped with known size genes in the budding yeast Saccharomyces cerevisiae. A potent size regulator specific to C. albicans was the conserved p38/HOG MAPK module that mediates the osmostress response. Basal HOG activity inhibited the SBF G1/S transcription factor complex in a stress-independent fashion to delay the G1/S transition. The HOG network also governed ribosome biogenesis through the master transcriptional regulator Sfp1. Hog1 bound to the promoters and cognate transcription factors for ribosome biogenesis regulons and interacted genetically with the SBF G1/S machinery, and thereby directly linked cell growth and division. These results illuminate the evolutionary plasticity of size control and identify the HOG module as a nexus of cell cycle and growth regulation. Public Library of Science 2019-03-28 /pmc/articles/PMC6456229/ /pubmed/30921326 http://dx.doi.org/10.1371/journal.pgen.1008052 Text en © 2019 Sellam et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Sellam, Adnane Chaillot, Julien Mallick, Jaideep Tebbji, Faiza Richard Albert, Julien Cook, Michael A. Tyers, Mike The p38/HOG stress-activated protein kinase network couples growth to division in Candida albicans |
title | The p38/HOG stress-activated protein kinase network couples growth to division in Candida albicans |
title_full | The p38/HOG stress-activated protein kinase network couples growth to division in Candida albicans |
title_fullStr | The p38/HOG stress-activated protein kinase network couples growth to division in Candida albicans |
title_full_unstemmed | The p38/HOG stress-activated protein kinase network couples growth to division in Candida albicans |
title_short | The p38/HOG stress-activated protein kinase network couples growth to division in Candida albicans |
title_sort | p38/hog stress-activated protein kinase network couples growth to division in candida albicans |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456229/ https://www.ncbi.nlm.nih.gov/pubmed/30921326 http://dx.doi.org/10.1371/journal.pgen.1008052 |
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