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Influenza A virus ribonucleoproteins form liquid organelles at endoplasmic reticulum exit sites

Influenza A virus has an eight-partite RNA genome that during viral assembly forms a complex containing one copy of each RNA. Genome assembly is a selective process driven by RNA-RNA interactions and is hypothesized to lead to discrete punctate structures scattered through the cytosol. Here, we show...

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Autores principales: Alenquer, Marta, Vale-Costa, Sílvia, Etibor, Temitope Akhigbe, Ferreira, Filipe, Sousa, Ana Laura, Amorim, Maria João
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456594/
https://www.ncbi.nlm.nih.gov/pubmed/30967547
http://dx.doi.org/10.1038/s41467-019-09549-4
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author Alenquer, Marta
Vale-Costa, Sílvia
Etibor, Temitope Akhigbe
Ferreira, Filipe
Sousa, Ana Laura
Amorim, Maria João
author_facet Alenquer, Marta
Vale-Costa, Sílvia
Etibor, Temitope Akhigbe
Ferreira, Filipe
Sousa, Ana Laura
Amorim, Maria João
author_sort Alenquer, Marta
collection PubMed
description Influenza A virus has an eight-partite RNA genome that during viral assembly forms a complex containing one copy of each RNA. Genome assembly is a selective process driven by RNA-RNA interactions and is hypothesized to lead to discrete punctate structures scattered through the cytosol. Here, we show that contrary to the accepted view, formation of these structures precedes RNA-RNA interactions among distinct viral ribonucleoproteins (vRNPs), as they assemble in cells expressing only one vRNP type. We demonstrate that these viral inclusions display characteristics of liquid organelles, segregating from the cytosol without a delimitating membrane, dynamically exchanging material and adapting fast to environmental changes. We provide evidence that viral inclusions develop close to endoplasmic reticulum (ER) exit sites, depend on continuous ER-Golgi vesicular cycling and do not promote escape to interferon response. We propose that viral inclusions segregate vRNPs from the cytosol and facilitate selected RNA-RNA interactions in a liquid environment.
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spelling pubmed-64565942019-04-11 Influenza A virus ribonucleoproteins form liquid organelles at endoplasmic reticulum exit sites Alenquer, Marta Vale-Costa, Sílvia Etibor, Temitope Akhigbe Ferreira, Filipe Sousa, Ana Laura Amorim, Maria João Nat Commun Article Influenza A virus has an eight-partite RNA genome that during viral assembly forms a complex containing one copy of each RNA. Genome assembly is a selective process driven by RNA-RNA interactions and is hypothesized to lead to discrete punctate structures scattered through the cytosol. Here, we show that contrary to the accepted view, formation of these structures precedes RNA-RNA interactions among distinct viral ribonucleoproteins (vRNPs), as they assemble in cells expressing only one vRNP type. We demonstrate that these viral inclusions display characteristics of liquid organelles, segregating from the cytosol without a delimitating membrane, dynamically exchanging material and adapting fast to environmental changes. We provide evidence that viral inclusions develop close to endoplasmic reticulum (ER) exit sites, depend on continuous ER-Golgi vesicular cycling and do not promote escape to interferon response. We propose that viral inclusions segregate vRNPs from the cytosol and facilitate selected RNA-RNA interactions in a liquid environment. Nature Publishing Group UK 2019-04-09 /pmc/articles/PMC6456594/ /pubmed/30967547 http://dx.doi.org/10.1038/s41467-019-09549-4 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Alenquer, Marta
Vale-Costa, Sílvia
Etibor, Temitope Akhigbe
Ferreira, Filipe
Sousa, Ana Laura
Amorim, Maria João
Influenza A virus ribonucleoproteins form liquid organelles at endoplasmic reticulum exit sites
title Influenza A virus ribonucleoproteins form liquid organelles at endoplasmic reticulum exit sites
title_full Influenza A virus ribonucleoproteins form liquid organelles at endoplasmic reticulum exit sites
title_fullStr Influenza A virus ribonucleoproteins form liquid organelles at endoplasmic reticulum exit sites
title_full_unstemmed Influenza A virus ribonucleoproteins form liquid organelles at endoplasmic reticulum exit sites
title_short Influenza A virus ribonucleoproteins form liquid organelles at endoplasmic reticulum exit sites
title_sort influenza a virus ribonucleoproteins form liquid organelles at endoplasmic reticulum exit sites
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456594/
https://www.ncbi.nlm.nih.gov/pubmed/30967547
http://dx.doi.org/10.1038/s41467-019-09549-4
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