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The effect of autophagy on the survival and invasive activity of Eimeria tenella sporozoites

Autophagy is a cellular process that is vital for the maintenance of homeostasis in eukaryotic cells. Currently, autophagy-related genes (atgs) in the Eimeria tenella genome database have been reported, but very little is known about the effects of autophagy on the survival and invasive activity of...

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Autores principales: Qi, Nanshan, Liao, Shenquan, Abuzeid, Asmaa M. I., Li, Juan, Wu, Caiyan, Lv, Minna, Lin, Xuhui, Hu, Junjing, Yu, Linzeng, Xiao, Wenwan, Sun, Mingfei, Li, Guoqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456608/
https://www.ncbi.nlm.nih.gov/pubmed/30967577
http://dx.doi.org/10.1038/s41598-019-41947-y
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author Qi, Nanshan
Liao, Shenquan
Abuzeid, Asmaa M. I.
Li, Juan
Wu, Caiyan
Lv, Minna
Lin, Xuhui
Hu, Junjing
Yu, Linzeng
Xiao, Wenwan
Sun, Mingfei
Li, Guoqing
author_facet Qi, Nanshan
Liao, Shenquan
Abuzeid, Asmaa M. I.
Li, Juan
Wu, Caiyan
Lv, Minna
Lin, Xuhui
Hu, Junjing
Yu, Linzeng
Xiao, Wenwan
Sun, Mingfei
Li, Guoqing
author_sort Qi, Nanshan
collection PubMed
description Autophagy is a cellular process that is vital for the maintenance of homeostasis in eukaryotic cells. Currently, autophagy-related genes (atgs) in the Eimeria tenella genome database have been reported, but very little is known about the effects of autophagy on the survival and invasive activity of this protozoan. In this study, we investigated the autophagy in E. tenella sporozoites under starvation and autophagy-modulators treatments and evaluated the autophagy influence on cellular adenosine triphosphate (ATP) levels, the survival rate and the invasive activity of the sporozoites. The results showed that the autophagy could be induced in the sporozoites by starvation or inducer rapamycin (RP), but it could be inhibited by 3-methyladenine (3-MA) treatment. The sporozoites after starvation and RP-treatment displayed punctate signals of EtATG8 and formed autophagosomes. The survival rate of the sporozoites under starvation was significantly lower than that in the control group, whereas the ATP levels in sporozoite were far greater than those in the control. The quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) showed that the invasive activity of the sporozoites was up- and down-regulated by RP and 3-MA induction, respectively. Our results indicate that autophagy has effects on the survival and invasive activity of E. tenella sporozoites, which may provide new insights into anti-coccidial drugs.
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spelling pubmed-64566082019-04-15 The effect of autophagy on the survival and invasive activity of Eimeria tenella sporozoites Qi, Nanshan Liao, Shenquan Abuzeid, Asmaa M. I. Li, Juan Wu, Caiyan Lv, Minna Lin, Xuhui Hu, Junjing Yu, Linzeng Xiao, Wenwan Sun, Mingfei Li, Guoqing Sci Rep Article Autophagy is a cellular process that is vital for the maintenance of homeostasis in eukaryotic cells. Currently, autophagy-related genes (atgs) in the Eimeria tenella genome database have been reported, but very little is known about the effects of autophagy on the survival and invasive activity of this protozoan. In this study, we investigated the autophagy in E. tenella sporozoites under starvation and autophagy-modulators treatments and evaluated the autophagy influence on cellular adenosine triphosphate (ATP) levels, the survival rate and the invasive activity of the sporozoites. The results showed that the autophagy could be induced in the sporozoites by starvation or inducer rapamycin (RP), but it could be inhibited by 3-methyladenine (3-MA) treatment. The sporozoites after starvation and RP-treatment displayed punctate signals of EtATG8 and formed autophagosomes. The survival rate of the sporozoites under starvation was significantly lower than that in the control group, whereas the ATP levels in sporozoite were far greater than those in the control. The quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) showed that the invasive activity of the sporozoites was up- and down-regulated by RP and 3-MA induction, respectively. Our results indicate that autophagy has effects on the survival and invasive activity of E. tenella sporozoites, which may provide new insights into anti-coccidial drugs. Nature Publishing Group UK 2019-04-09 /pmc/articles/PMC6456608/ /pubmed/30967577 http://dx.doi.org/10.1038/s41598-019-41947-y Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Qi, Nanshan
Liao, Shenquan
Abuzeid, Asmaa M. I.
Li, Juan
Wu, Caiyan
Lv, Minna
Lin, Xuhui
Hu, Junjing
Yu, Linzeng
Xiao, Wenwan
Sun, Mingfei
Li, Guoqing
The effect of autophagy on the survival and invasive activity of Eimeria tenella sporozoites
title The effect of autophagy on the survival and invasive activity of Eimeria tenella sporozoites
title_full The effect of autophagy on the survival and invasive activity of Eimeria tenella sporozoites
title_fullStr The effect of autophagy on the survival and invasive activity of Eimeria tenella sporozoites
title_full_unstemmed The effect of autophagy on the survival and invasive activity of Eimeria tenella sporozoites
title_short The effect of autophagy on the survival and invasive activity of Eimeria tenella sporozoites
title_sort effect of autophagy on the survival and invasive activity of eimeria tenella sporozoites
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456608/
https://www.ncbi.nlm.nih.gov/pubmed/30967577
http://dx.doi.org/10.1038/s41598-019-41947-y
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