β-carboline biomediators induce reveromycin production in Streptomyces sp. SN-593

The biosynthetic potential of soil-dwelling actinomycetes to produce diverse bioactive molecules that are useful as drug seeds has been achieved in the laboratory by modifying culture conditions. Availability of a small molecule that can induce secondary metabolism in these microbes can greatly faci...

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Autores principales: Panthee, Suresh, Takahashi, Shunji, Hayashi, Teruo, Shimizu, Takeshi, Osada, Hiroyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456619/
https://www.ncbi.nlm.nih.gov/pubmed/30967594
http://dx.doi.org/10.1038/s41598-019-42268-w
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author Panthee, Suresh
Takahashi, Shunji
Hayashi, Teruo
Shimizu, Takeshi
Osada, Hiroyuki
author_facet Panthee, Suresh
Takahashi, Shunji
Hayashi, Teruo
Shimizu, Takeshi
Osada, Hiroyuki
author_sort Panthee, Suresh
collection PubMed
description The biosynthetic potential of soil-dwelling actinomycetes to produce diverse bioactive molecules that are useful as drug seeds has been achieved in the laboratory by modifying culture conditions. Availability of a small molecule that can induce secondary metabolism in these microbes can greatly facilitate the exploration of bioactive natural products. In this manuscript, through the screening of natural products and chemical modification, we demonstrated that the presence of the β-carboline compound, BR-1, enhanced reveromycin A production in Streptomyces sp. SN-593. BR-1 induced reveromycins production at the wide range of concentrations without affecting cell growth. Our study indicates that BR-1 might serve as an alternative to activate specialized metabolite biosynthesis without genetic engineering.
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spelling pubmed-64566192019-04-15 β-carboline biomediators induce reveromycin production in Streptomyces sp. SN-593 Panthee, Suresh Takahashi, Shunji Hayashi, Teruo Shimizu, Takeshi Osada, Hiroyuki Sci Rep Article The biosynthetic potential of soil-dwelling actinomycetes to produce diverse bioactive molecules that are useful as drug seeds has been achieved in the laboratory by modifying culture conditions. Availability of a small molecule that can induce secondary metabolism in these microbes can greatly facilitate the exploration of bioactive natural products. In this manuscript, through the screening of natural products and chemical modification, we demonstrated that the presence of the β-carboline compound, BR-1, enhanced reveromycin A production in Streptomyces sp. SN-593. BR-1 induced reveromycins production at the wide range of concentrations without affecting cell growth. Our study indicates that BR-1 might serve as an alternative to activate specialized metabolite biosynthesis without genetic engineering. Nature Publishing Group UK 2019-04-09 /pmc/articles/PMC6456619/ /pubmed/30967594 http://dx.doi.org/10.1038/s41598-019-42268-w Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Panthee, Suresh
Takahashi, Shunji
Hayashi, Teruo
Shimizu, Takeshi
Osada, Hiroyuki
β-carboline biomediators induce reveromycin production in Streptomyces sp. SN-593
title β-carboline biomediators induce reveromycin production in Streptomyces sp. SN-593
title_full β-carboline biomediators induce reveromycin production in Streptomyces sp. SN-593
title_fullStr β-carboline biomediators induce reveromycin production in Streptomyces sp. SN-593
title_full_unstemmed β-carboline biomediators induce reveromycin production in Streptomyces sp. SN-593
title_short β-carboline biomediators induce reveromycin production in Streptomyces sp. SN-593
title_sort β-carboline biomediators induce reveromycin production in streptomyces sp. sn-593
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456619/
https://www.ncbi.nlm.nih.gov/pubmed/30967594
http://dx.doi.org/10.1038/s41598-019-42268-w
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