Cargando…

Fibronectin Functionalized Electrospun Fibers by Using Benign Solvents: Best Way to Achieve Effective Functionalization

The aim of this study is to demonstrate the feasibility of different functionalization methods for electrospun fibers developed using benign solvents. In particular three different approaches were investigated to achieve the functionalization of poly(epsilon caprolactone) (PCL) electrospun fibers wi...

Descripción completa

Detalles Bibliográficos
Autores principales: Liverani, Liliana, Killian, Manuela S., Boccaccini, Aldo R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456675/
https://www.ncbi.nlm.nih.gov/pubmed/31001528
http://dx.doi.org/10.3389/fbioe.2019.00068
_version_ 1783409785832472576
author Liverani, Liliana
Killian, Manuela S.
Boccaccini, Aldo R.
author_facet Liverani, Liliana
Killian, Manuela S.
Boccaccini, Aldo R.
author_sort Liverani, Liliana
collection PubMed
description The aim of this study is to demonstrate the feasibility of different functionalization methods for electrospun fibers developed using benign solvents. In particular three different approaches were investigated to achieve the functionalization of poly(epsilon caprolactone) (PCL) electrospun fibers with fibronectin. Protein surface entrapment, chemical functionalization and coaxial electrospinning were performed and compared. Moreover, bilayered scaffolds, with a top patterned and functionalized layer with fibronectin and a randomly oriented not functionalized layer were fabricated, demonstrating the versatility of the use of benign solvents for electrospinning also for the fabrication of complex graded structures. Besides the characterization of the morphology of the obtained scaffolds, ATR-FTIR and ToF-SIMS were used for the surface characterization of the functionalized fibers. Cell adhesion and proliferation were also investigated by using ST-2 cells. Positive results were obtained from all functionalized scaffolds and the most promising results were obtained with bilayered scaffolds, in terms of cells infiltration inside the fibrous structure.
format Online
Article
Text
id pubmed-6456675
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-64566752019-04-18 Fibronectin Functionalized Electrospun Fibers by Using Benign Solvents: Best Way to Achieve Effective Functionalization Liverani, Liliana Killian, Manuela S. Boccaccini, Aldo R. Front Bioeng Biotechnol Bioengineering and Biotechnology The aim of this study is to demonstrate the feasibility of different functionalization methods for electrospun fibers developed using benign solvents. In particular three different approaches were investigated to achieve the functionalization of poly(epsilon caprolactone) (PCL) electrospun fibers with fibronectin. Protein surface entrapment, chemical functionalization and coaxial electrospinning were performed and compared. Moreover, bilayered scaffolds, with a top patterned and functionalized layer with fibronectin and a randomly oriented not functionalized layer were fabricated, demonstrating the versatility of the use of benign solvents for electrospinning also for the fabrication of complex graded structures. Besides the characterization of the morphology of the obtained scaffolds, ATR-FTIR and ToF-SIMS were used for the surface characterization of the functionalized fibers. Cell adhesion and proliferation were also investigated by using ST-2 cells. Positive results were obtained from all functionalized scaffolds and the most promising results were obtained with bilayered scaffolds, in terms of cells infiltration inside the fibrous structure. Frontiers Media S.A. 2019-04-03 /pmc/articles/PMC6456675/ /pubmed/31001528 http://dx.doi.org/10.3389/fbioe.2019.00068 Text en Copyright © 2019 Liverani, Killian and Boccaccini. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Liverani, Liliana
Killian, Manuela S.
Boccaccini, Aldo R.
Fibronectin Functionalized Electrospun Fibers by Using Benign Solvents: Best Way to Achieve Effective Functionalization
title Fibronectin Functionalized Electrospun Fibers by Using Benign Solvents: Best Way to Achieve Effective Functionalization
title_full Fibronectin Functionalized Electrospun Fibers by Using Benign Solvents: Best Way to Achieve Effective Functionalization
title_fullStr Fibronectin Functionalized Electrospun Fibers by Using Benign Solvents: Best Way to Achieve Effective Functionalization
title_full_unstemmed Fibronectin Functionalized Electrospun Fibers by Using Benign Solvents: Best Way to Achieve Effective Functionalization
title_short Fibronectin Functionalized Electrospun Fibers by Using Benign Solvents: Best Way to Achieve Effective Functionalization
title_sort fibronectin functionalized electrospun fibers by using benign solvents: best way to achieve effective functionalization
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456675/
https://www.ncbi.nlm.nih.gov/pubmed/31001528
http://dx.doi.org/10.3389/fbioe.2019.00068
work_keys_str_mv AT liveranililiana fibronectinfunctionalizedelectrospunfibersbyusingbenignsolventsbestwaytoachieveeffectivefunctionalization
AT killianmanuelas fibronectinfunctionalizedelectrospunfibersbyusingbenignsolventsbestwaytoachieveeffectivefunctionalization
AT boccaccinialdor fibronectinfunctionalizedelectrospunfibersbyusingbenignsolventsbestwaytoachieveeffectivefunctionalization