StaPLs: versatile genetically encoded modules for engineering drug-inducible proteins

Robust approaches for chemogenetic control of protein function would enable many biological applications. We describe stabilizable polypeptide linkages (StaPLs) based on hepatitis C virus protease. StaPLs undergo autoproteolysis to cleave proteins by default, while protease inhibitors prevent cleava...

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Detalles Bibliográficos
Autores principales: Jacobs, Conor L., Badiee, Ryan K., Lin, Michael Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456726/
https://www.ncbi.nlm.nih.gov/pubmed/29967496
http://dx.doi.org/10.1038/s41592-018-0041-z
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author Jacobs, Conor L.
Badiee, Ryan K.
Lin, Michael Z.
author_facet Jacobs, Conor L.
Badiee, Ryan K.
Lin, Michael Z.
author_sort Jacobs, Conor L.
collection PubMed
description Robust approaches for chemogenetic control of protein function would enable many biological applications. We describe stabilizable polypeptide linkages (StaPLs) based on hepatitis C virus protease. StaPLs undergo autoproteolysis to cleave proteins by default, while protease inhibitors prevent cleavage and preserve protein function. We created StaPLs responsive to different clinically approved drugs to bidirectionally control transcription with zinc-finger-based effectors, and used StaPLs to create single-chain drug-stabilizable variants of CRISPR/Cas9 and caspase-9.
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spelling pubmed-64567262019-04-10 StaPLs: versatile genetically encoded modules for engineering drug-inducible proteins Jacobs, Conor L. Badiee, Ryan K. Lin, Michael Z. Nat Methods Article Robust approaches for chemogenetic control of protein function would enable many biological applications. We describe stabilizable polypeptide linkages (StaPLs) based on hepatitis C virus protease. StaPLs undergo autoproteolysis to cleave proteins by default, while protease inhibitors prevent cleavage and preserve protein function. We created StaPLs responsive to different clinically approved drugs to bidirectionally control transcription with zinc-finger-based effectors, and used StaPLs to create single-chain drug-stabilizable variants of CRISPR/Cas9 and caspase-9. 2018-07-02 2018-07 /pmc/articles/PMC6456726/ /pubmed/29967496 http://dx.doi.org/10.1038/s41592-018-0041-z Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Jacobs, Conor L.
Badiee, Ryan K.
Lin, Michael Z.
StaPLs: versatile genetically encoded modules for engineering drug-inducible proteins
title StaPLs: versatile genetically encoded modules for engineering drug-inducible proteins
title_full StaPLs: versatile genetically encoded modules for engineering drug-inducible proteins
title_fullStr StaPLs: versatile genetically encoded modules for engineering drug-inducible proteins
title_full_unstemmed StaPLs: versatile genetically encoded modules for engineering drug-inducible proteins
title_short StaPLs: versatile genetically encoded modules for engineering drug-inducible proteins
title_sort stapls: versatile genetically encoded modules for engineering drug-inducible proteins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456726/
https://www.ncbi.nlm.nih.gov/pubmed/29967496
http://dx.doi.org/10.1038/s41592-018-0041-z
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