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The inhibition of miR-126 in cell migration and invasion of cervical cancer through regulating ZEB1

BACKGROUND: Cervical cancer is a malignancy that’s common in female with high incidence and mortality worldwide. MicroRNAs (miRNAs) act a pivotal part in human cancer development. Our aim was to investigate the effect of miR-126 on cervical cancer and its underlying molecular mechanism. RESULTS: Fir...

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Autores principales: Xu, Jiqin, Wang, Hongyun, Wang, Huiyan, Chen, Qing, Zhang, Li, Song, Chao, Zhou, Qianqian, Hong, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456986/
https://www.ncbi.nlm.nih.gov/pubmed/31007650
http://dx.doi.org/10.1186/s41065-019-0087-7
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author Xu, Jiqin
Wang, Hongyun
Wang, Huiyan
Chen, Qing
Zhang, Li
Song, Chao
Zhou, Qianqian
Hong, Ying
author_facet Xu, Jiqin
Wang, Hongyun
Wang, Huiyan
Chen, Qing
Zhang, Li
Song, Chao
Zhou, Qianqian
Hong, Ying
author_sort Xu, Jiqin
collection PubMed
description BACKGROUND: Cervical cancer is a malignancy that’s common in female with high incidence and mortality worldwide. MicroRNAs (miRNAs) act a pivotal part in human cancer development. Our aim was to investigate the effect of miR-126 on cervical cancer and its underlying molecular mechanism. RESULTS: Firstly, RT-qPCR assay revealed that the expression of miR-126 was significantly downregulated in cervical cancer tissues and cell lines, compared with that in normal adjacent tissues and normal cervical epithelial cell line (Ect1/E6E7), respectively. Then, ZEB1 was verified as a target of miR-126 by using luciferase reporter assay. Inversely, the expression of ZEB1 was markedly upregulated in tumor tissues, and its mRNA level was negatively regulated by miR-126 expression in SiHa and Hela cells. Moreover, the capability of cell proliferation, migration and invasion was analyzed by CCK-8, wound healing assay and transwell assay, respectively. The results demonstrated that overexpression of miR-126 inhibited SiHa and Hela cell proliferation, migration and invasion, while ZEB1 abolished the inhibition induced by miR-126. Additionally, miR-126 suppressed MMP2 and MMP9 in mRNA and protein levels, as well as inhibited the protein expression of p-JAK2 and p-STAT3 in both SiHa and Hela cells, while ZEB1 rescued miR-126-induced suppression. CONCLUSION: miR-126 functions as a tumor suppressor in cervical cancer cells in vitro, which inhibits the proliferation, migration and invasion by suppressing MMP2, MMP9 expression and inactivating JAK2/STAT3 signaling pathway through targeting ZEB1, suggesting that miR-126 might be a novel potential target for the diagnosis and treatment of patients with cervical cancer.
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spelling pubmed-64569862019-04-19 The inhibition of miR-126 in cell migration and invasion of cervical cancer through regulating ZEB1 Xu, Jiqin Wang, Hongyun Wang, Huiyan Chen, Qing Zhang, Li Song, Chao Zhou, Qianqian Hong, Ying Hereditas Research BACKGROUND: Cervical cancer is a malignancy that’s common in female with high incidence and mortality worldwide. MicroRNAs (miRNAs) act a pivotal part in human cancer development. Our aim was to investigate the effect of miR-126 on cervical cancer and its underlying molecular mechanism. RESULTS: Firstly, RT-qPCR assay revealed that the expression of miR-126 was significantly downregulated in cervical cancer tissues and cell lines, compared with that in normal adjacent tissues and normal cervical epithelial cell line (Ect1/E6E7), respectively. Then, ZEB1 was verified as a target of miR-126 by using luciferase reporter assay. Inversely, the expression of ZEB1 was markedly upregulated in tumor tissues, and its mRNA level was negatively regulated by miR-126 expression in SiHa and Hela cells. Moreover, the capability of cell proliferation, migration and invasion was analyzed by CCK-8, wound healing assay and transwell assay, respectively. The results demonstrated that overexpression of miR-126 inhibited SiHa and Hela cell proliferation, migration and invasion, while ZEB1 abolished the inhibition induced by miR-126. Additionally, miR-126 suppressed MMP2 and MMP9 in mRNA and protein levels, as well as inhibited the protein expression of p-JAK2 and p-STAT3 in both SiHa and Hela cells, while ZEB1 rescued miR-126-induced suppression. CONCLUSION: miR-126 functions as a tumor suppressor in cervical cancer cells in vitro, which inhibits the proliferation, migration and invasion by suppressing MMP2, MMP9 expression and inactivating JAK2/STAT3 signaling pathway through targeting ZEB1, suggesting that miR-126 might be a novel potential target for the diagnosis and treatment of patients with cervical cancer. BioMed Central 2019-04-09 /pmc/articles/PMC6456986/ /pubmed/31007650 http://dx.doi.org/10.1186/s41065-019-0087-7 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Xu, Jiqin
Wang, Hongyun
Wang, Huiyan
Chen, Qing
Zhang, Li
Song, Chao
Zhou, Qianqian
Hong, Ying
The inhibition of miR-126 in cell migration and invasion of cervical cancer through regulating ZEB1
title The inhibition of miR-126 in cell migration and invasion of cervical cancer through regulating ZEB1
title_full The inhibition of miR-126 in cell migration and invasion of cervical cancer through regulating ZEB1
title_fullStr The inhibition of miR-126 in cell migration and invasion of cervical cancer through regulating ZEB1
title_full_unstemmed The inhibition of miR-126 in cell migration and invasion of cervical cancer through regulating ZEB1
title_short The inhibition of miR-126 in cell migration and invasion of cervical cancer through regulating ZEB1
title_sort inhibition of mir-126 in cell migration and invasion of cervical cancer through regulating zeb1
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456986/
https://www.ncbi.nlm.nih.gov/pubmed/31007650
http://dx.doi.org/10.1186/s41065-019-0087-7
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