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High-throughput characterisation of bull semen motility using differential dynamic microscopy
We report a high-throughput technique for characterising the motility of spermatozoa using differential dynamic microscopy. A movie with large field of view (∼10mm(2)) records thousands of cells (e.g. ≈ 5000 cells even at a low cell density of 20 × 10(6) cells/ml) at once and yields averaged measure...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6457493/ https://www.ncbi.nlm.nih.gov/pubmed/30969959 http://dx.doi.org/10.1371/journal.pone.0202720 |
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author | Jepson, Alys Arlt, Jochen Statham, Jonathan Spilman, Mark Burton, Katie Wood, Tiffany Poon, Wilson C. K. Martinez, Vincent A. |
author_facet | Jepson, Alys Arlt, Jochen Statham, Jonathan Spilman, Mark Burton, Katie Wood, Tiffany Poon, Wilson C. K. Martinez, Vincent A. |
author_sort | Jepson, Alys |
collection | PubMed |
description | We report a high-throughput technique for characterising the motility of spermatozoa using differential dynamic microscopy. A movie with large field of view (∼10mm(2)) records thousands of cells (e.g. ≈ 5000 cells even at a low cell density of 20 × 10(6) cells/ml) at once and yields averaged measurements of the mean ([Image: see text] ) and standard deviation (σ) of the swimming speed, head oscillation amplitude (A(0)) and frequency (f(0)), and the fraction of motile spermatozoa (α). Interestingly, we found that the measurement of α is facilitated because the swimming spermatozoa enhance the motion of the non-swimming population. We demonstrate the ease and rapidity of our method by performing on-farm characterisation of bull spermatozoa motility, and validate the technique by comparing laboratory measurements with tracking. Our results confirm the long-standing theoretical prediction that [Image: see text] for swimming spermatozoa. |
format | Online Article Text |
id | pubmed-6457493 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-64574932019-05-03 High-throughput characterisation of bull semen motility using differential dynamic microscopy Jepson, Alys Arlt, Jochen Statham, Jonathan Spilman, Mark Burton, Katie Wood, Tiffany Poon, Wilson C. K. Martinez, Vincent A. PLoS One Research Article We report a high-throughput technique for characterising the motility of spermatozoa using differential dynamic microscopy. A movie with large field of view (∼10mm(2)) records thousands of cells (e.g. ≈ 5000 cells even at a low cell density of 20 × 10(6) cells/ml) at once and yields averaged measurements of the mean ([Image: see text] ) and standard deviation (σ) of the swimming speed, head oscillation amplitude (A(0)) and frequency (f(0)), and the fraction of motile spermatozoa (α). Interestingly, we found that the measurement of α is facilitated because the swimming spermatozoa enhance the motion of the non-swimming population. We demonstrate the ease and rapidity of our method by performing on-farm characterisation of bull spermatozoa motility, and validate the technique by comparing laboratory measurements with tracking. Our results confirm the long-standing theoretical prediction that [Image: see text] for swimming spermatozoa. Public Library of Science 2019-04-10 /pmc/articles/PMC6457493/ /pubmed/30969959 http://dx.doi.org/10.1371/journal.pone.0202720 Text en © 2019 Jepson et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Jepson, Alys Arlt, Jochen Statham, Jonathan Spilman, Mark Burton, Katie Wood, Tiffany Poon, Wilson C. K. Martinez, Vincent A. High-throughput characterisation of bull semen motility using differential dynamic microscopy |
title | High-throughput characterisation of bull semen motility using differential dynamic microscopy |
title_full | High-throughput characterisation of bull semen motility using differential dynamic microscopy |
title_fullStr | High-throughput characterisation of bull semen motility using differential dynamic microscopy |
title_full_unstemmed | High-throughput characterisation of bull semen motility using differential dynamic microscopy |
title_short | High-throughput characterisation of bull semen motility using differential dynamic microscopy |
title_sort | high-throughput characterisation of bull semen motility using differential dynamic microscopy |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6457493/ https://www.ncbi.nlm.nih.gov/pubmed/30969959 http://dx.doi.org/10.1371/journal.pone.0202720 |
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