Cargando…

High-throughput characterisation of bull semen motility using differential dynamic microscopy

We report a high-throughput technique for characterising the motility of spermatozoa using differential dynamic microscopy. A movie with large field of view (∼10mm(2)) records thousands of cells (e.g. ≈ 5000 cells even at a low cell density of 20 × 10(6) cells/ml) at once and yields averaged measure...

Descripción completa

Detalles Bibliográficos
Autores principales: Jepson, Alys, Arlt, Jochen, Statham, Jonathan, Spilman, Mark, Burton, Katie, Wood, Tiffany, Poon, Wilson C. K., Martinez, Vincent A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6457493/
https://www.ncbi.nlm.nih.gov/pubmed/30969959
http://dx.doi.org/10.1371/journal.pone.0202720
_version_ 1783409909673492480
author Jepson, Alys
Arlt, Jochen
Statham, Jonathan
Spilman, Mark
Burton, Katie
Wood, Tiffany
Poon, Wilson C. K.
Martinez, Vincent A.
author_facet Jepson, Alys
Arlt, Jochen
Statham, Jonathan
Spilman, Mark
Burton, Katie
Wood, Tiffany
Poon, Wilson C. K.
Martinez, Vincent A.
author_sort Jepson, Alys
collection PubMed
description We report a high-throughput technique for characterising the motility of spermatozoa using differential dynamic microscopy. A movie with large field of view (∼10mm(2)) records thousands of cells (e.g. ≈ 5000 cells even at a low cell density of 20 × 10(6) cells/ml) at once and yields averaged measurements of the mean ([Image: see text] ) and standard deviation (σ) of the swimming speed, head oscillation amplitude (A(0)) and frequency (f(0)), and the fraction of motile spermatozoa (α). Interestingly, we found that the measurement of α is facilitated because the swimming spermatozoa enhance the motion of the non-swimming population. We demonstrate the ease and rapidity of our method by performing on-farm characterisation of bull spermatozoa motility, and validate the technique by comparing laboratory measurements with tracking. Our results confirm the long-standing theoretical prediction that [Image: see text] for swimming spermatozoa.
format Online
Article
Text
id pubmed-6457493
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-64574932019-05-03 High-throughput characterisation of bull semen motility using differential dynamic microscopy Jepson, Alys Arlt, Jochen Statham, Jonathan Spilman, Mark Burton, Katie Wood, Tiffany Poon, Wilson C. K. Martinez, Vincent A. PLoS One Research Article We report a high-throughput technique for characterising the motility of spermatozoa using differential dynamic microscopy. A movie with large field of view (∼10mm(2)) records thousands of cells (e.g. ≈ 5000 cells even at a low cell density of 20 × 10(6) cells/ml) at once and yields averaged measurements of the mean ([Image: see text] ) and standard deviation (σ) of the swimming speed, head oscillation amplitude (A(0)) and frequency (f(0)), and the fraction of motile spermatozoa (α). Interestingly, we found that the measurement of α is facilitated because the swimming spermatozoa enhance the motion of the non-swimming population. We demonstrate the ease and rapidity of our method by performing on-farm characterisation of bull spermatozoa motility, and validate the technique by comparing laboratory measurements with tracking. Our results confirm the long-standing theoretical prediction that [Image: see text] for swimming spermatozoa. Public Library of Science 2019-04-10 /pmc/articles/PMC6457493/ /pubmed/30969959 http://dx.doi.org/10.1371/journal.pone.0202720 Text en © 2019 Jepson et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Jepson, Alys
Arlt, Jochen
Statham, Jonathan
Spilman, Mark
Burton, Katie
Wood, Tiffany
Poon, Wilson C. K.
Martinez, Vincent A.
High-throughput characterisation of bull semen motility using differential dynamic microscopy
title High-throughput characterisation of bull semen motility using differential dynamic microscopy
title_full High-throughput characterisation of bull semen motility using differential dynamic microscopy
title_fullStr High-throughput characterisation of bull semen motility using differential dynamic microscopy
title_full_unstemmed High-throughput characterisation of bull semen motility using differential dynamic microscopy
title_short High-throughput characterisation of bull semen motility using differential dynamic microscopy
title_sort high-throughput characterisation of bull semen motility using differential dynamic microscopy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6457493/
https://www.ncbi.nlm.nih.gov/pubmed/30969959
http://dx.doi.org/10.1371/journal.pone.0202720
work_keys_str_mv AT jepsonalys highthroughputcharacterisationofbullsemenmotilityusingdifferentialdynamicmicroscopy
AT arltjochen highthroughputcharacterisationofbullsemenmotilityusingdifferentialdynamicmicroscopy
AT stathamjonathan highthroughputcharacterisationofbullsemenmotilityusingdifferentialdynamicmicroscopy
AT spilmanmark highthroughputcharacterisationofbullsemenmotilityusingdifferentialdynamicmicroscopy
AT burtonkatie highthroughputcharacterisationofbullsemenmotilityusingdifferentialdynamicmicroscopy
AT woodtiffany highthroughputcharacterisationofbullsemenmotilityusingdifferentialdynamicmicroscopy
AT poonwilsonck highthroughputcharacterisationofbullsemenmotilityusingdifferentialdynamicmicroscopy
AT martinezvincenta highthroughputcharacterisationofbullsemenmotilityusingdifferentialdynamicmicroscopy