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Structural and catalytic insights into HoLaMa, a derivative of Klenow DNA polymerase lacking the proofreading domain

We report here on the stability and catalytic properties of the HoLaMa DNA polymerase, a Klenow sub-fragment lacking the 3’-5’ exonuclease domain. HoLaMa was overexpressed in Escherichia coli, and the enzyme was purified by means of standard chromatographic techniques. High-resolution NMR experiment...

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Detalles Bibliográficos
Autores principales:	Kovermann, Michael, Stefan, Alessandra, Castaldo, Anna, Caramia, Sara, Hochkoeppler, Alejandro
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2019
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6457538/ https://www.ncbi.nlm.nih.gov/pubmed/30970012 http://dx.doi.org/10.1371/journal.pone.0215411

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