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High throughput DNA extraction of legume root nodules for rhizobial metagenomics
A novel combination of tissue homogenization, cell lysis, and DNA purification techniques was developed for isolating total DNA from whole legume root nodules on an automated robotic system. Silica dehydrated root nodules from soy bean, Glycine max cv. Tara, and mung bean, Vigna radiata cv. Crystal,...
Autor principal: | Johnson, Gabriel |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6458200/ https://www.ncbi.nlm.nih.gov/pubmed/30969386 http://dx.doi.org/10.1186/s13568-019-0771-z |
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