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Collembola interact with mycorrhizal fungi in modifying oak morphology, C and N incorporation and transcriptomics
Soil detritivores such as Collembola impact plant growth, tissue nutrient concentration and gene expression. Using a model system with pedunculate oak (Quercus robur) microcuttings that display a typical endogenous rhythmic growth with alternating shoot (SF) and root flushes (RF), we investigated th...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6458381/ https://www.ncbi.nlm.nih.gov/pubmed/31032040 http://dx.doi.org/10.1098/rsos.181869 |
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author | Graf, Marcel Bönn, Markus Feldhahn, Lasse Kurth, Florence Grams, Thorsten E. E. Herrmann, Sylvie Tarkka, Mika Buscot, Francois Scheu, Stefan |
author_facet | Graf, Marcel Bönn, Markus Feldhahn, Lasse Kurth, Florence Grams, Thorsten E. E. Herrmann, Sylvie Tarkka, Mika Buscot, Francois Scheu, Stefan |
author_sort | Graf, Marcel |
collection | PubMed |
description | Soil detritivores such as Collembola impact plant growth, tissue nutrient concentration and gene expression. Using a model system with pedunculate oak (Quercus robur) microcuttings that display a typical endogenous rhythmic growth with alternating shoot (SF) and root flushes (RF), we investigated the transcriptomic response of oak with and without mycorrhiza (Piloderma croceum) to the presence of Collembola (Protaphorura armata), and linked it to changes in resource allocation by pulse labelling the plants with (13)C and (15)N. Collembola impacted Gene Ontology (GO) terms as well as plant morphology and elemental ratios with the effects varying markedly with developmental phases. During SF Collembola increased GO terms related to primary growth and this was mirrored in increased (13)C and (15)N excess in aboveground plant compartments. During RF, Collembola increased GO terms related to plant secondary metabolism and physical fortification. Further, Collembola presence resulted in an increase in plant defence-related GO terms suggesting that Collembola in the rhizosphere prime oak shoots against the attack by fungi or herbivores. Notably, the impact of Collembola on growth, resource allocation and oak gene expression was modified by presence of P. croceum. The results indicate that oaks clearly react to the presence of Collembola in the rhizosphere and respond in a complex way by changing the expression of genes of both primary and secondary metabolism, and this resulted in concomitant changes in plant morphology and physiology. |
format | Online Article Text |
id | pubmed-6458381 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Royal Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-64583812019-04-26 Collembola interact with mycorrhizal fungi in modifying oak morphology, C and N incorporation and transcriptomics Graf, Marcel Bönn, Markus Feldhahn, Lasse Kurth, Florence Grams, Thorsten E. E. Herrmann, Sylvie Tarkka, Mika Buscot, Francois Scheu, Stefan R Soc Open Sci Biology (Whole Organism) Soil detritivores such as Collembola impact plant growth, tissue nutrient concentration and gene expression. Using a model system with pedunculate oak (Quercus robur) microcuttings that display a typical endogenous rhythmic growth with alternating shoot (SF) and root flushes (RF), we investigated the transcriptomic response of oak with and without mycorrhiza (Piloderma croceum) to the presence of Collembola (Protaphorura armata), and linked it to changes in resource allocation by pulse labelling the plants with (13)C and (15)N. Collembola impacted Gene Ontology (GO) terms as well as plant morphology and elemental ratios with the effects varying markedly with developmental phases. During SF Collembola increased GO terms related to primary growth and this was mirrored in increased (13)C and (15)N excess in aboveground plant compartments. During RF, Collembola increased GO terms related to plant secondary metabolism and physical fortification. Further, Collembola presence resulted in an increase in plant defence-related GO terms suggesting that Collembola in the rhizosphere prime oak shoots against the attack by fungi or herbivores. Notably, the impact of Collembola on growth, resource allocation and oak gene expression was modified by presence of P. croceum. The results indicate that oaks clearly react to the presence of Collembola in the rhizosphere and respond in a complex way by changing the expression of genes of both primary and secondary metabolism, and this resulted in concomitant changes in plant morphology and physiology. The Royal Society 2019-03-06 /pmc/articles/PMC6458381/ /pubmed/31032040 http://dx.doi.org/10.1098/rsos.181869 Text en © 2019 The Authors. http://creativecommons.org/licenses/by/4.0/ Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited. |
spellingShingle | Biology (Whole Organism) Graf, Marcel Bönn, Markus Feldhahn, Lasse Kurth, Florence Grams, Thorsten E. E. Herrmann, Sylvie Tarkka, Mika Buscot, Francois Scheu, Stefan Collembola interact with mycorrhizal fungi in modifying oak morphology, C and N incorporation and transcriptomics |
title | Collembola interact with mycorrhizal fungi in modifying oak morphology, C and N incorporation and transcriptomics |
title_full | Collembola interact with mycorrhizal fungi in modifying oak morphology, C and N incorporation and transcriptomics |
title_fullStr | Collembola interact with mycorrhizal fungi in modifying oak morphology, C and N incorporation and transcriptomics |
title_full_unstemmed | Collembola interact with mycorrhizal fungi in modifying oak morphology, C and N incorporation and transcriptomics |
title_short | Collembola interact with mycorrhizal fungi in modifying oak morphology, C and N incorporation and transcriptomics |
title_sort | collembola interact with mycorrhizal fungi in modifying oak morphology, c and n incorporation and transcriptomics |
topic | Biology (Whole Organism) |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6458381/ https://www.ncbi.nlm.nih.gov/pubmed/31032040 http://dx.doi.org/10.1098/rsos.181869 |
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