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Chronic amoxicillin exposure affects Labeo rohita: assessment of hematological, ionic compounds, biochemical, and enzymological activities

Labeo rohita were exposed to amoxicillin at a concentration of 1 mg/L (Treatment -I) and 0.5 mg/L (Treatment-II) for a period of 35 days. Numerous alterations were found in amoxicillin treatment groups when compared to the control group. Hemoglobin (Hb), hematocrit (Hct), and erythrocytes (RBCs) lev...

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Autores principales: Umamaheswari, Sathisaran, Renuka, Siva Shankar, Ramesh, Mathan, Poopal, Rama-Krishnan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6458497/
https://www.ncbi.nlm.nih.gov/pubmed/31008385
http://dx.doi.org/10.1016/j.heliyon.2019.e01434
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author Umamaheswari, Sathisaran
Renuka, Siva Shankar
Ramesh, Mathan
Poopal, Rama-Krishnan
author_facet Umamaheswari, Sathisaran
Renuka, Siva Shankar
Ramesh, Mathan
Poopal, Rama-Krishnan
author_sort Umamaheswari, Sathisaran
collection PubMed
description Labeo rohita were exposed to amoxicillin at a concentration of 1 mg/L (Treatment -I) and 0.5 mg/L (Treatment-II) for a period of 35 days. Numerous alterations were found in amoxicillin treatment groups when compared to the control group. Hemoglobin (Hb), hematocrit (Hct), and erythrocytes (RBCs) levels were significantly (P < 0.05) decreased. Leukocytes (WBC), mean cell volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) levels were significantly (P < 0.05) increased. In the plasma and gill tissues, ionic compounds (sodium, potassium, and chloride) levels were significantly declined throughout the treatment period. The plasma biochemical profiles were significantly altered: glucose level remained low (except at the end of 7(th) day in Treatment -I) till 35 days of the treatment period. Biphasic trend occurred in the protein level, significant increase was observed on 7(th) and 28(th) day (Treatment -I and -II), and 35(th) day (Treatment -I), and in remaining days its level was found to be decreased. Glutamate oxaloacetate transaminase (GOT) activity in the plasma was inhibited significantly, whereas in the gill, liver, and kidney tissues the enzyme activity was elevated. Plasma glutamate pyruvate transaminase (GPT) activity was inhibited throughout the study period. GPT activity in the gill was found to be elevated during the treatment period. Liver GPT activity was elevated in all the treatments except 28(th) (Treatment-I) and 35(th) day (Treatment-I, and II). GPT activity in the kidney was elevated (except 14(th) day in Treatment-II). Lactate dehydrogenase (LDH) activity was inhibited in plasma (except 14(th) day in Treatment-II), gill, liver (except 7(th) day in Treatment-I), and kidney tissues significantly (P < 0.05). The present study emphasizes that amoxicillin at 1 and 0.5 mg/L concentrations affects the hematological/biochemical/electrolytes/enzymological parameters of fish and these biomarkers serve as an effective test system for environmental risk assessment of pharmaceuticals in the aquatic environment.
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spelling pubmed-64584972019-04-19 Chronic amoxicillin exposure affects Labeo rohita: assessment of hematological, ionic compounds, biochemical, and enzymological activities Umamaheswari, Sathisaran Renuka, Siva Shankar Ramesh, Mathan Poopal, Rama-Krishnan Heliyon Article Labeo rohita were exposed to amoxicillin at a concentration of 1 mg/L (Treatment -I) and 0.5 mg/L (Treatment-II) for a period of 35 days. Numerous alterations were found in amoxicillin treatment groups when compared to the control group. Hemoglobin (Hb), hematocrit (Hct), and erythrocytes (RBCs) levels were significantly (P < 0.05) decreased. Leukocytes (WBC), mean cell volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) levels were significantly (P < 0.05) increased. In the plasma and gill tissues, ionic compounds (sodium, potassium, and chloride) levels were significantly declined throughout the treatment period. The plasma biochemical profiles were significantly altered: glucose level remained low (except at the end of 7(th) day in Treatment -I) till 35 days of the treatment period. Biphasic trend occurred in the protein level, significant increase was observed on 7(th) and 28(th) day (Treatment -I and -II), and 35(th) day (Treatment -I), and in remaining days its level was found to be decreased. Glutamate oxaloacetate transaminase (GOT) activity in the plasma was inhibited significantly, whereas in the gill, liver, and kidney tissues the enzyme activity was elevated. Plasma glutamate pyruvate transaminase (GPT) activity was inhibited throughout the study period. GPT activity in the gill was found to be elevated during the treatment period. Liver GPT activity was elevated in all the treatments except 28(th) (Treatment-I) and 35(th) day (Treatment-I, and II). GPT activity in the kidney was elevated (except 14(th) day in Treatment-II). Lactate dehydrogenase (LDH) activity was inhibited in plasma (except 14(th) day in Treatment-II), gill, liver (except 7(th) day in Treatment-I), and kidney tissues significantly (P < 0.05). The present study emphasizes that amoxicillin at 1 and 0.5 mg/L concentrations affects the hematological/biochemical/electrolytes/enzymological parameters of fish and these biomarkers serve as an effective test system for environmental risk assessment of pharmaceuticals in the aquatic environment. Elsevier 2019-04-09 /pmc/articles/PMC6458497/ /pubmed/31008385 http://dx.doi.org/10.1016/j.heliyon.2019.e01434 Text en © 2019 Published by Elsevier Ltd. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Umamaheswari, Sathisaran
Renuka, Siva Shankar
Ramesh, Mathan
Poopal, Rama-Krishnan
Chronic amoxicillin exposure affects Labeo rohita: assessment of hematological, ionic compounds, biochemical, and enzymological activities
title Chronic amoxicillin exposure affects Labeo rohita: assessment of hematological, ionic compounds, biochemical, and enzymological activities
title_full Chronic amoxicillin exposure affects Labeo rohita: assessment of hematological, ionic compounds, biochemical, and enzymological activities
title_fullStr Chronic amoxicillin exposure affects Labeo rohita: assessment of hematological, ionic compounds, biochemical, and enzymological activities
title_full_unstemmed Chronic amoxicillin exposure affects Labeo rohita: assessment of hematological, ionic compounds, biochemical, and enzymological activities
title_short Chronic amoxicillin exposure affects Labeo rohita: assessment of hematological, ionic compounds, biochemical, and enzymological activities
title_sort chronic amoxicillin exposure affects labeo rohita: assessment of hematological, ionic compounds, biochemical, and enzymological activities
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6458497/
https://www.ncbi.nlm.nih.gov/pubmed/31008385
http://dx.doi.org/10.1016/j.heliyon.2019.e01434
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