Enhanced extracellular expression of Bacillus stearothermophilus α-amylase in Bacillus subtilis through signal peptide optimization, chaperone overexpression and α-amylase mutant selection

BACKGROUND: Our laboratory has constructed a Bacillus stearothermophilus α-amylase (AmyS) derivative with excellent enzymatic properties. Bacillus subtilis is generally regarded as safe and has excellent protein secretory capability, but heterologous extracellular production level of B. stearothermo...

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Detalles Bibliográficos
Autores principales: Yao, Dongbang, Su, Lingqia, Li, Na, Wu, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6458788/
https://www.ncbi.nlm.nih.gov/pubmed/30971250
http://dx.doi.org/10.1186/s12934-019-1119-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6458788/
https://www.ncbi.nlm.nih.gov/pubmed/30971250
http://dx.doi.org/10.1186/s12934-019-1119-8

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