Cargando…

The Alcohol Extract of Coreopsis tinctoria Nutt Ameliorates Diabetes and Diabetic Nephropathy in db/db Mice through miR-192/miR-200b and PTEN/AKT and ZEB2/ECM Pathways

The study aims to investigate the effects of the alcohol extract of Coreopsis tinctoria Nutt (AC) on diabetic nephropathy (DN) mice. A total of 30 db/db (DN) mice were divided into 3 groups, which were treated with AC (300 mg/kg/day), metformin (180 mg/kg/day), or saline by gavage for 10 weeks. Ten...

Descripción completa

Detalles Bibliográficos
Autores principales: Yu, Shunjie, Zhao, Haoran, Yang, Wenjing, Amat, Ramila, Peng, Jun, Li, Yike, Deng, Kai, Mao, Xinmin, Jiao, Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6458854/
https://www.ncbi.nlm.nih.gov/pubmed/31032350
http://dx.doi.org/10.1155/2019/5280514
_version_ 1783410098622693376
author Yu, Shunjie
Zhao, Haoran
Yang, Wenjing
Amat, Ramila
Peng, Jun
Li, Yike
Deng, Kai
Mao, Xinmin
Jiao, Yi
author_facet Yu, Shunjie
Zhao, Haoran
Yang, Wenjing
Amat, Ramila
Peng, Jun
Li, Yike
Deng, Kai
Mao, Xinmin
Jiao, Yi
author_sort Yu, Shunjie
collection PubMed
description The study aims to investigate the effects of the alcohol extract of Coreopsis tinctoria Nutt (AC) on diabetic nephropathy (DN) mice. A total of 30 db/db (DN) mice were divided into 3 groups, which were treated with AC (300 mg/kg/day), metformin (180 mg/kg/day), or saline by gavage for 10 weeks. Ten db/m mice treated with saline were used as normal control (NC group). Body weight (BW) and fasting blood glucose (FBG), HbA1c, 24 h urinary albumin excretion (UAE), and renal pathological fibrosis were analyzed. Expression of miR-192, miR-200b, and proteins in the PTEN/PI3K/AKT pathway was analyzed by qPCR or western blot. The DN mice had significantly higher BW, FBG, and 24 h UAE, as well as more severe pathological fibrosis when compared with NC. Treatment of AC could decrease BW, FBG, and 24 h UAE and alleviated kidney damage. Compared with the NC group, expressions of miR-192 and miR-200b were increased, whereas their target proteins (ZEB2 and PTEN) were reduced in the kidneys of DN mice, which further modulated the expression of their downstream proteins PI3K p85α, P-AKT, P-smad3, and COL4 α1; these proteins were increased in the kidneys of DN mice. In contrast, AC treatment reversed the expression changes of these proteins. These findings demonstrate that AC may protect the kidneys of DN mice by decreasing miR-192 and miR-200b, which could further regulate their target gene expression and modulate the activity of the PTEN/PI3K/AKT pathway to reduce the degree of renal fibrosis.
format Online
Article
Text
id pubmed-6458854
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-64588542019-04-28 The Alcohol Extract of Coreopsis tinctoria Nutt Ameliorates Diabetes and Diabetic Nephropathy in db/db Mice through miR-192/miR-200b and PTEN/AKT and ZEB2/ECM Pathways Yu, Shunjie Zhao, Haoran Yang, Wenjing Amat, Ramila Peng, Jun Li, Yike Deng, Kai Mao, Xinmin Jiao, Yi Biomed Res Int Research Article The study aims to investigate the effects of the alcohol extract of Coreopsis tinctoria Nutt (AC) on diabetic nephropathy (DN) mice. A total of 30 db/db (DN) mice were divided into 3 groups, which were treated with AC (300 mg/kg/day), metformin (180 mg/kg/day), or saline by gavage for 10 weeks. Ten db/m mice treated with saline were used as normal control (NC group). Body weight (BW) and fasting blood glucose (FBG), HbA1c, 24 h urinary albumin excretion (UAE), and renal pathological fibrosis were analyzed. Expression of miR-192, miR-200b, and proteins in the PTEN/PI3K/AKT pathway was analyzed by qPCR or western blot. The DN mice had significantly higher BW, FBG, and 24 h UAE, as well as more severe pathological fibrosis when compared with NC. Treatment of AC could decrease BW, FBG, and 24 h UAE and alleviated kidney damage. Compared with the NC group, expressions of miR-192 and miR-200b were increased, whereas their target proteins (ZEB2 and PTEN) were reduced in the kidneys of DN mice, which further modulated the expression of their downstream proteins PI3K p85α, P-AKT, P-smad3, and COL4 α1; these proteins were increased in the kidneys of DN mice. In contrast, AC treatment reversed the expression changes of these proteins. These findings demonstrate that AC may protect the kidneys of DN mice by decreasing miR-192 and miR-200b, which could further regulate their target gene expression and modulate the activity of the PTEN/PI3K/AKT pathway to reduce the degree of renal fibrosis. Hindawi 2019-03-28 /pmc/articles/PMC6458854/ /pubmed/31032350 http://dx.doi.org/10.1155/2019/5280514 Text en Copyright © 2019 Shunjie Yu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yu, Shunjie
Zhao, Haoran
Yang, Wenjing
Amat, Ramila
Peng, Jun
Li, Yike
Deng, Kai
Mao, Xinmin
Jiao, Yi
The Alcohol Extract of Coreopsis tinctoria Nutt Ameliorates Diabetes and Diabetic Nephropathy in db/db Mice through miR-192/miR-200b and PTEN/AKT and ZEB2/ECM Pathways
title The Alcohol Extract of Coreopsis tinctoria Nutt Ameliorates Diabetes and Diabetic Nephropathy in db/db Mice through miR-192/miR-200b and PTEN/AKT and ZEB2/ECM Pathways
title_full The Alcohol Extract of Coreopsis tinctoria Nutt Ameliorates Diabetes and Diabetic Nephropathy in db/db Mice through miR-192/miR-200b and PTEN/AKT and ZEB2/ECM Pathways
title_fullStr The Alcohol Extract of Coreopsis tinctoria Nutt Ameliorates Diabetes and Diabetic Nephropathy in db/db Mice through miR-192/miR-200b and PTEN/AKT and ZEB2/ECM Pathways
title_full_unstemmed The Alcohol Extract of Coreopsis tinctoria Nutt Ameliorates Diabetes and Diabetic Nephropathy in db/db Mice through miR-192/miR-200b and PTEN/AKT and ZEB2/ECM Pathways
title_short The Alcohol Extract of Coreopsis tinctoria Nutt Ameliorates Diabetes and Diabetic Nephropathy in db/db Mice through miR-192/miR-200b and PTEN/AKT and ZEB2/ECM Pathways
title_sort alcohol extract of coreopsis tinctoria nutt ameliorates diabetes and diabetic nephropathy in db/db mice through mir-192/mir-200b and pten/akt and zeb2/ecm pathways
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6458854/
https://www.ncbi.nlm.nih.gov/pubmed/31032350
http://dx.doi.org/10.1155/2019/5280514
work_keys_str_mv AT yushunjie thealcoholextractofcoreopsistinctorianuttamelioratesdiabetesanddiabeticnephropathyindbdbmicethroughmir192mir200bandptenaktandzeb2ecmpathways
AT zhaohaoran thealcoholextractofcoreopsistinctorianuttamelioratesdiabetesanddiabeticnephropathyindbdbmicethroughmir192mir200bandptenaktandzeb2ecmpathways
AT yangwenjing thealcoholextractofcoreopsistinctorianuttamelioratesdiabetesanddiabeticnephropathyindbdbmicethroughmir192mir200bandptenaktandzeb2ecmpathways
AT amatramila thealcoholextractofcoreopsistinctorianuttamelioratesdiabetesanddiabeticnephropathyindbdbmicethroughmir192mir200bandptenaktandzeb2ecmpathways
AT pengjun thealcoholextractofcoreopsistinctorianuttamelioratesdiabetesanddiabeticnephropathyindbdbmicethroughmir192mir200bandptenaktandzeb2ecmpathways
AT liyike thealcoholextractofcoreopsistinctorianuttamelioratesdiabetesanddiabeticnephropathyindbdbmicethroughmir192mir200bandptenaktandzeb2ecmpathways
AT dengkai thealcoholextractofcoreopsistinctorianuttamelioratesdiabetesanddiabeticnephropathyindbdbmicethroughmir192mir200bandptenaktandzeb2ecmpathways
AT maoxinmin thealcoholextractofcoreopsistinctorianuttamelioratesdiabetesanddiabeticnephropathyindbdbmicethroughmir192mir200bandptenaktandzeb2ecmpathways
AT jiaoyi thealcoholextractofcoreopsistinctorianuttamelioratesdiabetesanddiabeticnephropathyindbdbmicethroughmir192mir200bandptenaktandzeb2ecmpathways
AT yushunjie alcoholextractofcoreopsistinctorianuttamelioratesdiabetesanddiabeticnephropathyindbdbmicethroughmir192mir200bandptenaktandzeb2ecmpathways
AT zhaohaoran alcoholextractofcoreopsistinctorianuttamelioratesdiabetesanddiabeticnephropathyindbdbmicethroughmir192mir200bandptenaktandzeb2ecmpathways
AT yangwenjing alcoholextractofcoreopsistinctorianuttamelioratesdiabetesanddiabeticnephropathyindbdbmicethroughmir192mir200bandptenaktandzeb2ecmpathways
AT amatramila alcoholextractofcoreopsistinctorianuttamelioratesdiabetesanddiabeticnephropathyindbdbmicethroughmir192mir200bandptenaktandzeb2ecmpathways
AT pengjun alcoholextractofcoreopsistinctorianuttamelioratesdiabetesanddiabeticnephropathyindbdbmicethroughmir192mir200bandptenaktandzeb2ecmpathways
AT liyike alcoholextractofcoreopsistinctorianuttamelioratesdiabetesanddiabeticnephropathyindbdbmicethroughmir192mir200bandptenaktandzeb2ecmpathways
AT dengkai alcoholextractofcoreopsistinctorianuttamelioratesdiabetesanddiabeticnephropathyindbdbmicethroughmir192mir200bandptenaktandzeb2ecmpathways
AT maoxinmin alcoholextractofcoreopsistinctorianuttamelioratesdiabetesanddiabeticnephropathyindbdbmicethroughmir192mir200bandptenaktandzeb2ecmpathways
AT jiaoyi alcoholextractofcoreopsistinctorianuttamelioratesdiabetesanddiabeticnephropathyindbdbmicethroughmir192mir200bandptenaktandzeb2ecmpathways