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Root canal dressings for revascularization influence in vitro mineralization of apical papilla cells

Endodontic revascularization is based on cell recruitment into the necrotic root canal of immature teeth after chemical disinfection. The clinical outcome depends on the ability of surviving cells from the apical tissue to differentiate and promote hard tissue deposition inside the dentinal walls. O...

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Autores principales: RAHHAL, Juliana Garuba, ROVAI, Emanuel da Silva, HOLZHAUSEN, Marinella, CALDEIRA, Celso Luiz, dos SANTOS, Carlos Ferreira, SIPERT, Carla Renata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Faculdade De Odontologia De Bauru - USP 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6459230/
https://www.ncbi.nlm.nih.gov/pubmed/30994774
http://dx.doi.org/10.1590/1678-7757-2018-0396
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author RAHHAL, Juliana Garuba
ROVAI, Emanuel da Silva
HOLZHAUSEN, Marinella
CALDEIRA, Celso Luiz
dos SANTOS, Carlos Ferreira
SIPERT, Carla Renata
author_facet RAHHAL, Juliana Garuba
ROVAI, Emanuel da Silva
HOLZHAUSEN, Marinella
CALDEIRA, Celso Luiz
dos SANTOS, Carlos Ferreira
SIPERT, Carla Renata
author_sort RAHHAL, Juliana Garuba
collection PubMed
description Endodontic revascularization is based on cell recruitment into the necrotic root canal of immature teeth after chemical disinfection. The clinical outcome depends on the ability of surviving cells from the apical tissue to differentiate and promote hard tissue deposition inside the dentinal walls. OBJECTIVE: To investigate the effect of calcium hydroxide (CH) and modified triple antibiotic paste (mTAP – ciprofloxacin, metronidazole and cefaclor) on the viability and mineralization potential of apical papilla cells (APC) in vitro . MATERIAL AND METHODS: APC cultures were kept in contact with CH or mTAP (250-1000 µg/mL) for 5 days, after which cell viability was assessed using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Next, APCs were subjected to CH or mTAP at 250 µg/mL for 5 days before inducing the differentiation assay. After 14 and 21 days, calcium deposition was assessed by the Alizarin Red S staining method, followed by elution and quantification using spectrophotometry. Data were analyzed using ANOVA followed by Tukey post hoc test. RESULTS: CH induced cell proliferation, whereas mTAP showed significant cytotoxicity at all concentrations tested. APC treated with CH demonstrated improved mineralization capacity at 14 days, while, for mTAP, significant reduction on the mineralization rate was observed for both experimental periods (14 and 21 days). CONCLUSION: Our findings showed that CH induces cell proliferation and improves early mineralization, whereas mTAP was found cytotoxic and reduced the mineralization potential in vitro of APCs.
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spelling pubmed-64592302019-05-01 Root canal dressings for revascularization influence in vitro mineralization of apical papilla cells RAHHAL, Juliana Garuba ROVAI, Emanuel da Silva HOLZHAUSEN, Marinella CALDEIRA, Celso Luiz dos SANTOS, Carlos Ferreira SIPERT, Carla Renata J Appl Oral Sci Original Article Endodontic revascularization is based on cell recruitment into the necrotic root canal of immature teeth after chemical disinfection. The clinical outcome depends on the ability of surviving cells from the apical tissue to differentiate and promote hard tissue deposition inside the dentinal walls. OBJECTIVE: To investigate the effect of calcium hydroxide (CH) and modified triple antibiotic paste (mTAP – ciprofloxacin, metronidazole and cefaclor) on the viability and mineralization potential of apical papilla cells (APC) in vitro . MATERIAL AND METHODS: APC cultures were kept in contact with CH or mTAP (250-1000 µg/mL) for 5 days, after which cell viability was assessed using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Next, APCs were subjected to CH or mTAP at 250 µg/mL for 5 days before inducing the differentiation assay. After 14 and 21 days, calcium deposition was assessed by the Alizarin Red S staining method, followed by elution and quantification using spectrophotometry. Data were analyzed using ANOVA followed by Tukey post hoc test. RESULTS: CH induced cell proliferation, whereas mTAP showed significant cytotoxicity at all concentrations tested. APC treated with CH demonstrated improved mineralization capacity at 14 days, while, for mTAP, significant reduction on the mineralization rate was observed for both experimental periods (14 and 21 days). CONCLUSION: Our findings showed that CH induces cell proliferation and improves early mineralization, whereas mTAP was found cytotoxic and reduced the mineralization potential in vitro of APCs. Faculdade De Odontologia De Bauru - USP 2019-04-11 /pmc/articles/PMC6459230/ /pubmed/30994774 http://dx.doi.org/10.1590/1678-7757-2018-0396 Text en https://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
RAHHAL, Juliana Garuba
ROVAI, Emanuel da Silva
HOLZHAUSEN, Marinella
CALDEIRA, Celso Luiz
dos SANTOS, Carlos Ferreira
SIPERT, Carla Renata
Root canal dressings for revascularization influence in vitro mineralization of apical papilla cells
title Root canal dressings for revascularization influence in vitro mineralization of apical papilla cells
title_full Root canal dressings for revascularization influence in vitro mineralization of apical papilla cells
title_fullStr Root canal dressings for revascularization influence in vitro mineralization of apical papilla cells
title_full_unstemmed Root canal dressings for revascularization influence in vitro mineralization of apical papilla cells
title_short Root canal dressings for revascularization influence in vitro mineralization of apical papilla cells
title_sort root canal dressings for revascularization influence in vitro mineralization of apical papilla cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6459230/
https://www.ncbi.nlm.nih.gov/pubmed/30994774
http://dx.doi.org/10.1590/1678-7757-2018-0396
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