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Suppressor of fused controls perinatal expansion and quiescence of future dentate adult neural stem cells

Adult hippocampal neurogenesis requires the quiescent neural stem cell (NSC) pool to persist lifelong. However, establishment and maintenance of quiescent NSC pools during development is not understood. Here, we show that Suppressor of Fused (Sufu) controls establishment of the quiescent NSC pool du...

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Autores principales: Noguchi, Hirofumi, Castillo, Jesse Garcia, Nakashima, Kinichi, Pleasure, Samuel J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6459675/
https://www.ncbi.nlm.nih.gov/pubmed/30973324
http://dx.doi.org/10.7554/eLife.42918
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author Noguchi, Hirofumi
Castillo, Jesse Garcia
Nakashima, Kinichi
Pleasure, Samuel J
author_facet Noguchi, Hirofumi
Castillo, Jesse Garcia
Nakashima, Kinichi
Pleasure, Samuel J
author_sort Noguchi, Hirofumi
collection PubMed
description Adult hippocampal neurogenesis requires the quiescent neural stem cell (NSC) pool to persist lifelong. However, establishment and maintenance of quiescent NSC pools during development is not understood. Here, we show that Suppressor of Fused (Sufu) controls establishment of the quiescent NSC pool during mouse dentate gyrus (DG) development by regulating Sonic Hedgehog (Shh) signaling activity. Deletion of Sufu in NSCs early in DG development decreases Shh signaling activity leading to reduced proliferation of NSCs, resulting in a small quiescent NSC pool in adult mice. We found that putative adult NSCs proliferate and increase their numbers in the first postnatal week and subsequently enter a quiescent state towards the end of the first postnatal week. In the absence of Sufu, postnatal expansion of NSCs is compromised, and NSCs prematurely become quiescent. Thus, Sufu is required for Shh signaling activity ensuring expansion and proper transition of NSC pools to quiescent states during DG development.
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spelling pubmed-64596752019-04-16 Suppressor of fused controls perinatal expansion and quiescence of future dentate adult neural stem cells Noguchi, Hirofumi Castillo, Jesse Garcia Nakashima, Kinichi Pleasure, Samuel J eLife Developmental Biology Adult hippocampal neurogenesis requires the quiescent neural stem cell (NSC) pool to persist lifelong. However, establishment and maintenance of quiescent NSC pools during development is not understood. Here, we show that Suppressor of Fused (Sufu) controls establishment of the quiescent NSC pool during mouse dentate gyrus (DG) development by regulating Sonic Hedgehog (Shh) signaling activity. Deletion of Sufu in NSCs early in DG development decreases Shh signaling activity leading to reduced proliferation of NSCs, resulting in a small quiescent NSC pool in adult mice. We found that putative adult NSCs proliferate and increase their numbers in the first postnatal week and subsequently enter a quiescent state towards the end of the first postnatal week. In the absence of Sufu, postnatal expansion of NSCs is compromised, and NSCs prematurely become quiescent. Thus, Sufu is required for Shh signaling activity ensuring expansion and proper transition of NSC pools to quiescent states during DG development. eLife Sciences Publications, Ltd 2019-04-11 /pmc/articles/PMC6459675/ /pubmed/30973324 http://dx.doi.org/10.7554/eLife.42918 Text en © 2019, Noguchi et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Developmental Biology
Noguchi, Hirofumi
Castillo, Jesse Garcia
Nakashima, Kinichi
Pleasure, Samuel J
Suppressor of fused controls perinatal expansion and quiescence of future dentate adult neural stem cells
title Suppressor of fused controls perinatal expansion and quiescence of future dentate adult neural stem cells
title_full Suppressor of fused controls perinatal expansion and quiescence of future dentate adult neural stem cells
title_fullStr Suppressor of fused controls perinatal expansion and quiescence of future dentate adult neural stem cells
title_full_unstemmed Suppressor of fused controls perinatal expansion and quiescence of future dentate adult neural stem cells
title_short Suppressor of fused controls perinatal expansion and quiescence of future dentate adult neural stem cells
title_sort suppressor of fused controls perinatal expansion and quiescence of future dentate adult neural stem cells
topic Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6459675/
https://www.ncbi.nlm.nih.gov/pubmed/30973324
http://dx.doi.org/10.7554/eLife.42918
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