A new type of DNA phosphorothioation-based antiviral system in archaea

Archaea and Bacteria have evolved different defence strategies that target virtually all steps of the viral life cycle. The diversified virion morphotypes and genome contents of archaeal viruses result in a highly complex array of archaea-virus interactions. However, our understanding of archaeal an...

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Autores principales: Xiong, Lei, Liu, Siyi, Chen, Si, Xiao, Yao, Zhu, Bochen, Gao, Yali, Zhang, Yujing, Chen, Beibei, Luo, Jie, Deng, Zixin, Chen, Xiangdong, Wang, Lianrong, Chen, Shi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6459918/
https://www.ncbi.nlm.nih.gov/pubmed/30975999
http://dx.doi.org/10.1038/s41467-019-09390-9
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author Xiong, Lei
Liu, Siyi
Chen, Si
Xiao, Yao
Zhu, Bochen
Gao, Yali
Zhang, Yujing
Chen, Beibei
Luo, Jie
Deng, Zixin
Chen, Xiangdong
Wang, Lianrong
Chen, Shi
author_facet Xiong, Lei
Liu, Siyi
Chen, Si
Xiao, Yao
Zhu, Bochen
Gao, Yali
Zhang, Yujing
Chen, Beibei
Luo, Jie
Deng, Zixin
Chen, Xiangdong
Wang, Lianrong
Chen, Shi
author_sort Xiong, Lei
collection PubMed
description Archaea and Bacteria have evolved different defence strategies that target virtually all steps of the viral life cycle. The diversified virion morphotypes and genome contents of archaeal viruses result in a highly complex array of archaea-virus interactions. However, our understanding of archaeal antiviral activities lags far behind our knowledges of those in bacteria. Here we report a new archaeal defence system that involves DndCDEA-specific DNA phosphorothioate (PT) modification and the PbeABCD-mediated halt of virus propagation via inhibition of DNA replication. In contrast to the breakage of invasive DNA by DndFGH in bacteria, DndCDEA-PbeABCD does not degrade or cleave viral DNA. The PbeABCD-mediated PT defence system is widespread and exhibits extensive interdomain and intradomain gene transfer events. Our results suggest that DndCDEA-PbeABCD is a new type of PT-based virus resistance system, expanding the known arsenal of defence systems as well as our understanding of host-virus interactions.
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spelling pubmed-64599182019-04-15 A new type of DNA phosphorothioation-based antiviral system in archaea Xiong, Lei Liu, Siyi Chen, Si Xiao, Yao Zhu, Bochen Gao, Yali Zhang, Yujing Chen, Beibei Luo, Jie Deng, Zixin Chen, Xiangdong Wang, Lianrong Chen, Shi Nat Commun Article Archaea and Bacteria have evolved different defence strategies that target virtually all steps of the viral life cycle. The diversified virion morphotypes and genome contents of archaeal viruses result in a highly complex array of archaea-virus interactions. However, our understanding of archaeal antiviral activities lags far behind our knowledges of those in bacteria. Here we report a new archaeal defence system that involves DndCDEA-specific DNA phosphorothioate (PT) modification and the PbeABCD-mediated halt of virus propagation via inhibition of DNA replication. In contrast to the breakage of invasive DNA by DndFGH in bacteria, DndCDEA-PbeABCD does not degrade or cleave viral DNA. The PbeABCD-mediated PT defence system is widespread and exhibits extensive interdomain and intradomain gene transfer events. Our results suggest that DndCDEA-PbeABCD is a new type of PT-based virus resistance system, expanding the known arsenal of defence systems as well as our understanding of host-virus interactions. Nature Publishing Group UK 2019-04-11 /pmc/articles/PMC6459918/ /pubmed/30975999 http://dx.doi.org/10.1038/s41467-019-09390-9 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Xiong, Lei
Liu, Siyi
Chen, Si
Xiao, Yao
Zhu, Bochen
Gao, Yali
Zhang, Yujing
Chen, Beibei
Luo, Jie
Deng, Zixin
Chen, Xiangdong
Wang, Lianrong
Chen, Shi
A new type of DNA phosphorothioation-based antiviral system in archaea
title A new type of DNA phosphorothioation-based antiviral system in archaea
title_full A new type of DNA phosphorothioation-based antiviral system in archaea
title_fullStr A new type of DNA phosphorothioation-based antiviral system in archaea
title_full_unstemmed A new type of DNA phosphorothioation-based antiviral system in archaea
title_short A new type of DNA phosphorothioation-based antiviral system in archaea
title_sort new type of dna phosphorothioation-based antiviral system in archaea
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6459918/
https://www.ncbi.nlm.nih.gov/pubmed/30975999
http://dx.doi.org/10.1038/s41467-019-09390-9
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