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A Rapid Method for Detection of Salmonella in Milk Based on Extraction of mRNA Using Magnetic Capture Probes and RT-qPCR
Magnetic separation is an efficient method for target enrichment and elimination of inhibitors in the molecular detection systems for foodborne pathogens. In this study, we prepared magnetic capture probes by modifying oligonucleotides complementary to target sequences on the surface of amino-modifi...
Autores principales: | Bai, Yalong, Cui, Yan, Suo, Yujuan, Shi, Chunlei, Wang, Dapeng, Shi, Xianming |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6459957/ https://www.ncbi.nlm.nih.gov/pubmed/31024515 http://dx.doi.org/10.3389/fmicb.2019.00770 |
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