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Complete genome sequence of natural rubber-degrading, gram-negative bacterium, Rhizobacter gummiphilus strain NS21(T)

Gram-negative natural rubber-degrader, Rhizobacter gummiphilus NS21(T), which was isolated from soil in the botanical garden in Japan, is a newly proposed species of genus of Rhizobacter. It has been reported that the latA1 gene is involved in the natural rubber degradation in this strain. To gain n...

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Autores principales: Linh, Dao Viet, Gibu, Namiko, Tabata, Michiro, Imai, Shunsuke, Hosoyama, Akira, Yamazoe, Atsushi, Kasai, Daisuke, Fukuda, Masao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6460296/
https://www.ncbi.nlm.nih.gov/pubmed/31011550
http://dx.doi.org/10.1016/j.btre.2019.e00332
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author Linh, Dao Viet
Gibu, Namiko
Tabata, Michiro
Imai, Shunsuke
Hosoyama, Akira
Yamazoe, Atsushi
Kasai, Daisuke
Fukuda, Masao
author_facet Linh, Dao Viet
Gibu, Namiko
Tabata, Michiro
Imai, Shunsuke
Hosoyama, Akira
Yamazoe, Atsushi
Kasai, Daisuke
Fukuda, Masao
author_sort Linh, Dao Viet
collection PubMed
description Gram-negative natural rubber-degrader, Rhizobacter gummiphilus NS21(T), which was isolated from soil in the botanical garden in Japan, is a newly proposed species of genus of Rhizobacter. It has been reported that the latA1 gene is involved in the natural rubber degradation in this strain. To gain novel insights into natural rubber degradation pathway, the complete genome sequence of this strain was determined. The genome of strain NS21(T) consists of 6,398,096 bp of circular chromosome (GenBank accession number CP015118.1) with G + C content of 69.72%. The genome contains 5687 protein-coding and 68 RNA genes. Among the predicted genes, 4810 genes were categorized as functional COGs. Homology search revealed that existence of latA1 homologous gene (latA2) in this genome. Quantitative reverse-transcription-PCR and deletion analyses indicated that natural rubber degradation of this strain requires latA2 as well as latA1.
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spelling pubmed-64602962019-04-22 Complete genome sequence of natural rubber-degrading, gram-negative bacterium, Rhizobacter gummiphilus strain NS21(T) Linh, Dao Viet Gibu, Namiko Tabata, Michiro Imai, Shunsuke Hosoyama, Akira Yamazoe, Atsushi Kasai, Daisuke Fukuda, Masao Biotechnol Rep (Amst) Article Gram-negative natural rubber-degrader, Rhizobacter gummiphilus NS21(T), which was isolated from soil in the botanical garden in Japan, is a newly proposed species of genus of Rhizobacter. It has been reported that the latA1 gene is involved in the natural rubber degradation in this strain. To gain novel insights into natural rubber degradation pathway, the complete genome sequence of this strain was determined. The genome of strain NS21(T) consists of 6,398,096 bp of circular chromosome (GenBank accession number CP015118.1) with G + C content of 69.72%. The genome contains 5687 protein-coding and 68 RNA genes. Among the predicted genes, 4810 genes were categorized as functional COGs. Homology search revealed that existence of latA1 homologous gene (latA2) in this genome. Quantitative reverse-transcription-PCR and deletion analyses indicated that natural rubber degradation of this strain requires latA2 as well as latA1. Elsevier 2019-04-01 /pmc/articles/PMC6460296/ /pubmed/31011550 http://dx.doi.org/10.1016/j.btre.2019.e00332 Text en © 2019 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Linh, Dao Viet
Gibu, Namiko
Tabata, Michiro
Imai, Shunsuke
Hosoyama, Akira
Yamazoe, Atsushi
Kasai, Daisuke
Fukuda, Masao
Complete genome sequence of natural rubber-degrading, gram-negative bacterium, Rhizobacter gummiphilus strain NS21(T)
title Complete genome sequence of natural rubber-degrading, gram-negative bacterium, Rhizobacter gummiphilus strain NS21(T)
title_full Complete genome sequence of natural rubber-degrading, gram-negative bacterium, Rhizobacter gummiphilus strain NS21(T)
title_fullStr Complete genome sequence of natural rubber-degrading, gram-negative bacterium, Rhizobacter gummiphilus strain NS21(T)
title_full_unstemmed Complete genome sequence of natural rubber-degrading, gram-negative bacterium, Rhizobacter gummiphilus strain NS21(T)
title_short Complete genome sequence of natural rubber-degrading, gram-negative bacterium, Rhizobacter gummiphilus strain NS21(T)
title_sort complete genome sequence of natural rubber-degrading, gram-negative bacterium, rhizobacter gummiphilus strain ns21(t)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6460296/
https://www.ncbi.nlm.nih.gov/pubmed/31011550
http://dx.doi.org/10.1016/j.btre.2019.e00332
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