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Bone Marrow and Peripheral Blood Cells Toxicity of a Single 2.0 Gy Cobalt(60) Ionizing Radiation: An Animal Model

BACKGROUND: Bone marrow is extremely vulnerable to damage caused by radiation therapy. Hence, bone marrow suppression is an important side effect of radiotherapy. Effective use of radiotherapy is therefore compromised by radiation-related injuries. MATERIAL AND METHODS: Six Guinea-pigs were recruite...

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Autores principales: Akeem, Shittu, Lukman, Olatunbosun, Eltahir, Khalil, Fatai, Olalere, Abiola, Babatunde, Khadijat, Omokanye
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Research and Publications Office of Jimma University 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6460448/
https://www.ncbi.nlm.nih.gov/pubmed/31011267
http://dx.doi.org/10.4314/ejhs.v29i2.6
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author Akeem, Shittu
Lukman, Olatunbosun
Eltahir, Khalil
Fatai, Olalere
Abiola, Babatunde
Khadijat, Omokanye
author_facet Akeem, Shittu
Lukman, Olatunbosun
Eltahir, Khalil
Fatai, Olalere
Abiola, Babatunde
Khadijat, Omokanye
author_sort Akeem, Shittu
collection PubMed
description BACKGROUND: Bone marrow is extremely vulnerable to damage caused by radiation therapy. Hence, bone marrow suppression is an important side effect of radiotherapy. Effective use of radiotherapy is therefore compromised by radiation-related injuries. MATERIAL AND METHODS: Six Guinea-pigs were recruited for the study of which three were subjected to total body irradiation with Co(60) while the other three served as controls. Bone marrow and peripheral blood samples were collected before and at days 9, 14 and 21, post irradiation. Manual and automated counts were performed for bone marrow nucleated cells and peripheral blood cells respectively. RESULTS: Declining bone marrow cellularity was evident immediately post irradiation. Mean ± SD of marrow cell counted per mm(3) were 121,924±281, 87,603±772, 121,367±375 and 122,750±1000 pre-irradiation and days 9, 14 and 21, postirradiation (p-values 0.10, 0.27 and 0.29 respectively). Significant drops in counts were noticed on day 9 post-irradiation for all red cell parameters (p-values <0.05), for Total White Blood Cell Count and Neutrophil count (p-values <0.05) and also on days 14 and 21 for Lymphocytes (p-values <0.05) and on day 21 for Eosinophil/Basophil/Monocytes (p-value <0.05). A significant drop in platelets counts was also noticed on day 9 (p-value <0.05) which significantly increased above pre-irradiation value on day 21. CONCLUSION: Total body irrradiation with Co(60) significantly affects the bone marrow with maximum reductions in marrow nucleated cells and peripheral blood cells counts on day 9 post irradiation.
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spelling pubmed-64604482019-04-22 Bone Marrow and Peripheral Blood Cells Toxicity of a Single 2.0 Gy Cobalt(60) Ionizing Radiation: An Animal Model Akeem, Shittu Lukman, Olatunbosun Eltahir, Khalil Fatai, Olalere Abiola, Babatunde Khadijat, Omokanye Ethiop J Health Sci Original Article BACKGROUND: Bone marrow is extremely vulnerable to damage caused by radiation therapy. Hence, bone marrow suppression is an important side effect of radiotherapy. Effective use of radiotherapy is therefore compromised by radiation-related injuries. MATERIAL AND METHODS: Six Guinea-pigs were recruited for the study of which three were subjected to total body irradiation with Co(60) while the other three served as controls. Bone marrow and peripheral blood samples were collected before and at days 9, 14 and 21, post irradiation. Manual and automated counts were performed for bone marrow nucleated cells and peripheral blood cells respectively. RESULTS: Declining bone marrow cellularity was evident immediately post irradiation. Mean ± SD of marrow cell counted per mm(3) were 121,924±281, 87,603±772, 121,367±375 and 122,750±1000 pre-irradiation and days 9, 14 and 21, postirradiation (p-values 0.10, 0.27 and 0.29 respectively). Significant drops in counts were noticed on day 9 post-irradiation for all red cell parameters (p-values <0.05), for Total White Blood Cell Count and Neutrophil count (p-values <0.05) and also on days 14 and 21 for Lymphocytes (p-values <0.05) and on day 21 for Eosinophil/Basophil/Monocytes (p-value <0.05). A significant drop in platelets counts was also noticed on day 9 (p-value <0.05) which significantly increased above pre-irradiation value on day 21. CONCLUSION: Total body irrradiation with Co(60) significantly affects the bone marrow with maximum reductions in marrow nucleated cells and peripheral blood cells counts on day 9 post irradiation. Research and Publications Office of Jimma University 2019-03 /pmc/articles/PMC6460448/ /pubmed/31011267 http://dx.doi.org/10.4314/ejhs.v29i2.6 Text en © 2019 Shittu, A., et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Original Article
Akeem, Shittu
Lukman, Olatunbosun
Eltahir, Khalil
Fatai, Olalere
Abiola, Babatunde
Khadijat, Omokanye
Bone Marrow and Peripheral Blood Cells Toxicity of a Single 2.0 Gy Cobalt(60) Ionizing Radiation: An Animal Model
title Bone Marrow and Peripheral Blood Cells Toxicity of a Single 2.0 Gy Cobalt(60) Ionizing Radiation: An Animal Model
title_full Bone Marrow and Peripheral Blood Cells Toxicity of a Single 2.0 Gy Cobalt(60) Ionizing Radiation: An Animal Model
title_fullStr Bone Marrow and Peripheral Blood Cells Toxicity of a Single 2.0 Gy Cobalt(60) Ionizing Radiation: An Animal Model
title_full_unstemmed Bone Marrow and Peripheral Blood Cells Toxicity of a Single 2.0 Gy Cobalt(60) Ionizing Radiation: An Animal Model
title_short Bone Marrow and Peripheral Blood Cells Toxicity of a Single 2.0 Gy Cobalt(60) Ionizing Radiation: An Animal Model
title_sort bone marrow and peripheral blood cells toxicity of a single 2.0 gy cobalt(60) ionizing radiation: an animal model
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6460448/
https://www.ncbi.nlm.nih.gov/pubmed/31011267
http://dx.doi.org/10.4314/ejhs.v29i2.6
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