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The in vitro behaviour of canine osteoblasts derived from different bone types

BACKGROUND: Our understanding of the biology of osteoblasts is important as they underpin bone remodelling, fracture healing and processes such as osseointegration. Osteoblasts isolated from human humeral samples display distinctive biological activity in vitro, which relates to the samples’ bone ty...

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Autores principales: Meeson, Richard L., Perpétuo, Inês P., Parsons, Kevin, Orriss, Isabel R., Shah, Mittal, Pitsillides, Andrew A., Doube, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6460523/
https://www.ncbi.nlm.nih.gov/pubmed/30975149
http://dx.doi.org/10.1186/s12917-019-1857-1
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author Meeson, Richard L.
Perpétuo, Inês P.
Parsons, Kevin
Orriss, Isabel R.
Shah, Mittal
Pitsillides, Andrew A.
Doube, Michael
author_facet Meeson, Richard L.
Perpétuo, Inês P.
Parsons, Kevin
Orriss, Isabel R.
Shah, Mittal
Pitsillides, Andrew A.
Doube, Michael
author_sort Meeson, Richard L.
collection PubMed
description BACKGROUND: Our understanding of the biology of osteoblasts is important as they underpin bone remodelling, fracture healing and processes such as osseointegration. Osteoblasts isolated from human humeral samples display distinctive biological activity in vitro, which relates to the samples’ bone types (subchondral (S), trabecular (T), cortical (C)). Our aim was to isolate primary osteoblast cultures from different bone types from the proximal femur of a clinical population of dogs presented for total hip replacement and compare the behaviour of the osteoblasts derived from different bone types, to identify a preferred bone type for isolation. RESULTS: No differences were found for osteoblast doubling time (median for S = 2.9, T = 3.1 and C = 2.71 days, respectively; p = 0.33), final cell number (median for S = 54,849, T = 49,733, C = 61,390 cells/cm(2); p = 0.34) or basal tissue non-specific alkaline phosphatase (TNAP) activity (median for S = 0.02, T = 0.02, C = 0.03 U/min/mg protein; p = 0.81) between bone types after 6 days of culture in basal media. There were no differences in mineralizing TNAP activity (S = 0.02, T = 0.02, C = 0.03 U/min/mg protein, p = 0.84) or in mineralized area (S = 0.05, T = 0.04, C = 0.04%, p = 0.92) among cells from different bone types. CONCLUSIONS: There is no significant difference in mean doubling time, basal or mineralizing TNAP activity or mineralized area in osteoblasts derived from subchondral, cortical, or trabecular bone types from the canine femoral head. However, there appears to be a high level of inter-animal variability in the studied parameters, which was independent of age, body mass, and sex. Trabecular isolate osteoblasts have the least variation of the bone types studied, and therefore should be considered a preferred source for primary osteoblast cultures. The work here provides baselines for canine osteoblast function, which has utility for future comparative studies.
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spelling pubmed-64605232019-04-22 The in vitro behaviour of canine osteoblasts derived from different bone types Meeson, Richard L. Perpétuo, Inês P. Parsons, Kevin Orriss, Isabel R. Shah, Mittal Pitsillides, Andrew A. Doube, Michael BMC Vet Res Research Article BACKGROUND: Our understanding of the biology of osteoblasts is important as they underpin bone remodelling, fracture healing and processes such as osseointegration. Osteoblasts isolated from human humeral samples display distinctive biological activity in vitro, which relates to the samples’ bone types (subchondral (S), trabecular (T), cortical (C)). Our aim was to isolate primary osteoblast cultures from different bone types from the proximal femur of a clinical population of dogs presented for total hip replacement and compare the behaviour of the osteoblasts derived from different bone types, to identify a preferred bone type for isolation. RESULTS: No differences were found for osteoblast doubling time (median for S = 2.9, T = 3.1 and C = 2.71 days, respectively; p = 0.33), final cell number (median for S = 54,849, T = 49,733, C = 61,390 cells/cm(2); p = 0.34) or basal tissue non-specific alkaline phosphatase (TNAP) activity (median for S = 0.02, T = 0.02, C = 0.03 U/min/mg protein; p = 0.81) between bone types after 6 days of culture in basal media. There were no differences in mineralizing TNAP activity (S = 0.02, T = 0.02, C = 0.03 U/min/mg protein, p = 0.84) or in mineralized area (S = 0.05, T = 0.04, C = 0.04%, p = 0.92) among cells from different bone types. CONCLUSIONS: There is no significant difference in mean doubling time, basal or mineralizing TNAP activity or mineralized area in osteoblasts derived from subchondral, cortical, or trabecular bone types from the canine femoral head. However, there appears to be a high level of inter-animal variability in the studied parameters, which was independent of age, body mass, and sex. Trabecular isolate osteoblasts have the least variation of the bone types studied, and therefore should be considered a preferred source for primary osteoblast cultures. The work here provides baselines for canine osteoblast function, which has utility for future comparative studies. BioMed Central 2019-04-11 /pmc/articles/PMC6460523/ /pubmed/30975149 http://dx.doi.org/10.1186/s12917-019-1857-1 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Meeson, Richard L.
Perpétuo, Inês P.
Parsons, Kevin
Orriss, Isabel R.
Shah, Mittal
Pitsillides, Andrew A.
Doube, Michael
The in vitro behaviour of canine osteoblasts derived from different bone types
title The in vitro behaviour of canine osteoblasts derived from different bone types
title_full The in vitro behaviour of canine osteoblasts derived from different bone types
title_fullStr The in vitro behaviour of canine osteoblasts derived from different bone types
title_full_unstemmed The in vitro behaviour of canine osteoblasts derived from different bone types
title_short The in vitro behaviour of canine osteoblasts derived from different bone types
title_sort in vitro behaviour of canine osteoblasts derived from different bone types
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6460523/
https://www.ncbi.nlm.nih.gov/pubmed/30975149
http://dx.doi.org/10.1186/s12917-019-1857-1
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