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Efficient introduction of an isogenic homozygous mutation to induced pluripotent stem cells from a hereditary hearing loss family using CRISPR/Cas9 and single-stranded donor oligonucleotides

BACKGROUND: Heterozygous purinergic receptor p2x gene (P2RX2) c.178G>T (p.V60L) mutations can lead to progressive hearing loss (HL) and increased susceptibility to noise. However, the underlying mechanisms remain unclear. A combination of human induced pluripotent stem cell (hiPSC) technology wit...

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Detalles Bibliográficos
Autores principales:	Dong, Yunpeng, Peng, Tao, Wu, Weijing, Tan, Donghui, Liu, Xuezhong, Xie, Dinghua
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	SAGE Publications 2019
Materias:	Pre-Clinical Research Reports
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6460615/ https://www.ncbi.nlm.nih.gov/pubmed/30819013 http://dx.doi.org/10.1177/0300060519829990

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