Cargando…

Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution

The beneficial effects of silicon (Si) have been shown on plants using reduction-based strategy for iron (Fe) acquisition. Here we investigated the influence of Si on Fe deficiency stress alleviation in barley (Hordeum vulgare), a crop plant which uses the chelation-based strategy for Fe acquisition...

Descripción completa

Detalles Bibliográficos
Autores principales: Nikolic, Dragana B., Nesic, Sofija, Bosnic, Dragana, Kostic, Ljiljana, Nikolic, Miroslav, Samardzic, Jelena T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6460936/
https://www.ncbi.nlm.nih.gov/pubmed/31024590
http://dx.doi.org/10.3389/fpls.2019.00416
_version_ 1783410409409085440
author Nikolic, Dragana B.
Nesic, Sofija
Bosnic, Dragana
Kostic, Ljiljana
Nikolic, Miroslav
Samardzic, Jelena T.
author_facet Nikolic, Dragana B.
Nesic, Sofija
Bosnic, Dragana
Kostic, Ljiljana
Nikolic, Miroslav
Samardzic, Jelena T.
author_sort Nikolic, Dragana B.
collection PubMed
description The beneficial effects of silicon (Si) have been shown on plants using reduction-based strategy for iron (Fe) acquisition. Here we investigated the influence of Si on Fe deficiency stress alleviation in barley (Hordeum vulgare), a crop plant which uses the chelation-based strategy for Fe acquisition. Analyses of chlorophyll content, ROS accumulation, antioxidative status, concentrations of Fe and other micronutrients, along with the expression of Strategy II genes were studied in response to Si supply. Si successfully ameliorated Fe deficiency in barley, diminishing chlorophyll and biomass loss, and improving the activity of antioxidative enzymes, resulting in lowered reactive oxidative species accumulation in the youngest leaves. Alleviation of Fe deficiency stress correlated well with the Si-induced increase of Fe content in the youngest leaves, while it was decreased in root. Moreover, Si nutrition lowered accumulation of other micronutrients in the youngest leaves of Fe deprived plants, by retaining them in the root. On the transcriptional level, Si led to an expedient increase in the expression of genes involved in Strategy II Fe acquisition in roots at the early stage of Fe deficiency stress, while decreasing their expression in a prolonged stress response. Expression of Strategy II genes was remarkably upregulated in the leaves of Si supplied plants. This study broadens the perspective of mechanisms of Si action, providing evidence for ameliorative effects of Si on Strategy II plants, including its influence on accumulation and distribution of microelements, as well as on the expression of the Strategy II genes.
format Online
Article
Text
id pubmed-6460936
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-64609362019-04-25 Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution Nikolic, Dragana B. Nesic, Sofija Bosnic, Dragana Kostic, Ljiljana Nikolic, Miroslav Samardzic, Jelena T. Front Plant Sci Plant Science The beneficial effects of silicon (Si) have been shown on plants using reduction-based strategy for iron (Fe) acquisition. Here we investigated the influence of Si on Fe deficiency stress alleviation in barley (Hordeum vulgare), a crop plant which uses the chelation-based strategy for Fe acquisition. Analyses of chlorophyll content, ROS accumulation, antioxidative status, concentrations of Fe and other micronutrients, along with the expression of Strategy II genes were studied in response to Si supply. Si successfully ameliorated Fe deficiency in barley, diminishing chlorophyll and biomass loss, and improving the activity of antioxidative enzymes, resulting in lowered reactive oxidative species accumulation in the youngest leaves. Alleviation of Fe deficiency stress correlated well with the Si-induced increase of Fe content in the youngest leaves, while it was decreased in root. Moreover, Si nutrition lowered accumulation of other micronutrients in the youngest leaves of Fe deprived plants, by retaining them in the root. On the transcriptional level, Si led to an expedient increase in the expression of genes involved in Strategy II Fe acquisition in roots at the early stage of Fe deficiency stress, while decreasing their expression in a prolonged stress response. Expression of Strategy II genes was remarkably upregulated in the leaves of Si supplied plants. This study broadens the perspective of mechanisms of Si action, providing evidence for ameliorative effects of Si on Strategy II plants, including its influence on accumulation and distribution of microelements, as well as on the expression of the Strategy II genes. Frontiers Media S.A. 2019-04-05 /pmc/articles/PMC6460936/ /pubmed/31024590 http://dx.doi.org/10.3389/fpls.2019.00416 Text en Copyright © 2019 Nikolic, Nesic, Bosnic, Kostic, Nikolic and Samardzic. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Nikolic, Dragana B.
Nesic, Sofija
Bosnic, Dragana
Kostic, Ljiljana
Nikolic, Miroslav
Samardzic, Jelena T.
Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution
title Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution
title_full Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution
title_fullStr Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution
title_full_unstemmed Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution
title_short Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution
title_sort silicon alleviates iron deficiency in barley by enhancing expression of strategy ii genes and metal redistribution
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6460936/
https://www.ncbi.nlm.nih.gov/pubmed/31024590
http://dx.doi.org/10.3389/fpls.2019.00416
work_keys_str_mv AT nikolicdraganab siliconalleviatesirondeficiencyinbarleybyenhancingexpressionofstrategyiigenesandmetalredistribution
AT nesicsofija siliconalleviatesirondeficiencyinbarleybyenhancingexpressionofstrategyiigenesandmetalredistribution
AT bosnicdragana siliconalleviatesirondeficiencyinbarleybyenhancingexpressionofstrategyiigenesandmetalredistribution
AT kosticljiljana siliconalleviatesirondeficiencyinbarleybyenhancingexpressionofstrategyiigenesandmetalredistribution
AT nikolicmiroslav siliconalleviatesirondeficiencyinbarleybyenhancingexpressionofstrategyiigenesandmetalredistribution
AT samardzicjelenat siliconalleviatesirondeficiencyinbarleybyenhancingexpressionofstrategyiigenesandmetalredistribution