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Comprehensive analysis of Lon proteases in plants highlights independent gene duplication events
The degradation of damaged proteins is essential for cell viability. Lon is a highly conserved ATP-dependent serine-lysine protease that maintains proteostasis. We performed a comparative genome-wide analysis to determine the evolutionary history of Lon proteases. Prokaryotes and unicellular eukaryo...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6460959/ https://www.ncbi.nlm.nih.gov/pubmed/30590727 http://dx.doi.org/10.1093/jxb/ery440 |
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author | Tsitsekian, Dikran Daras, Gerasimos Alatzas, Anastasios Templalexis, Dimitris Hatzopoulos, Polydefkis Rigas, Stamatis |
author_facet | Tsitsekian, Dikran Daras, Gerasimos Alatzas, Anastasios Templalexis, Dimitris Hatzopoulos, Polydefkis Rigas, Stamatis |
author_sort | Tsitsekian, Dikran |
collection | PubMed |
description | The degradation of damaged proteins is essential for cell viability. Lon is a highly conserved ATP-dependent serine-lysine protease that maintains proteostasis. We performed a comparative genome-wide analysis to determine the evolutionary history of Lon proteases. Prokaryotes and unicellular eukaryotes retained a single Lon copy, whereas multicellular eukaryotes acquired a peroxisomal copy, in addition to the mitochondrial gene, to sustain the evolution of higher order organ structures. Land plants developed small Lon gene families. Despite the Lon2 peroxisomal paralog, Lon genes triplicated in the Arabidopsis lineage through sequential evolutionary events including whole-genome and tandem duplications. The retention of Lon1, Lon4, and Lon3 triplicates relied on their differential and even contrasting expression patterns, distinct subcellular targeting mechanisms, and functional divergence. Lon1 seems similar to the pre-duplication ancestral gene unit, whereas the duplication of Lon3 and Lon4 is evolutionarily recent. In the wider context of plant evolution, papaya is the only genome with a single ancestral Lon1-type gene. The evolutionary trend among plants is to acquire Lon copies with ambiguous pre-sequences for dual-targeting to mitochondria and chloroplasts, and a substrate recognition domain that deviates from the ancestral Lon1 type. Lon genes constitute a paradigm of dynamic evolution contributing to understanding the functional fate of gene duplicates. |
format | Online Article Text |
id | pubmed-6460959 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-64609592019-04-17 Comprehensive analysis of Lon proteases in plants highlights independent gene duplication events Tsitsekian, Dikran Daras, Gerasimos Alatzas, Anastasios Templalexis, Dimitris Hatzopoulos, Polydefkis Rigas, Stamatis J Exp Bot Research Papers The degradation of damaged proteins is essential for cell viability. Lon is a highly conserved ATP-dependent serine-lysine protease that maintains proteostasis. We performed a comparative genome-wide analysis to determine the evolutionary history of Lon proteases. Prokaryotes and unicellular eukaryotes retained a single Lon copy, whereas multicellular eukaryotes acquired a peroxisomal copy, in addition to the mitochondrial gene, to sustain the evolution of higher order organ structures. Land plants developed small Lon gene families. Despite the Lon2 peroxisomal paralog, Lon genes triplicated in the Arabidopsis lineage through sequential evolutionary events including whole-genome and tandem duplications. The retention of Lon1, Lon4, and Lon3 triplicates relied on their differential and even contrasting expression patterns, distinct subcellular targeting mechanisms, and functional divergence. Lon1 seems similar to the pre-duplication ancestral gene unit, whereas the duplication of Lon3 and Lon4 is evolutionarily recent. In the wider context of plant evolution, papaya is the only genome with a single ancestral Lon1-type gene. The evolutionary trend among plants is to acquire Lon copies with ambiguous pre-sequences for dual-targeting to mitochondria and chloroplasts, and a substrate recognition domain that deviates from the ancestral Lon1 type. Lon genes constitute a paradigm of dynamic evolution contributing to understanding the functional fate of gene duplicates. Oxford University Press 2019-03-15 2018-12-24 /pmc/articles/PMC6460959/ /pubmed/30590727 http://dx.doi.org/10.1093/jxb/ery440 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Papers Tsitsekian, Dikran Daras, Gerasimos Alatzas, Anastasios Templalexis, Dimitris Hatzopoulos, Polydefkis Rigas, Stamatis Comprehensive analysis of Lon proteases in plants highlights independent gene duplication events |
title | Comprehensive analysis of Lon proteases in plants highlights independent gene duplication events |
title_full | Comprehensive analysis of Lon proteases in plants highlights independent gene duplication events |
title_fullStr | Comprehensive analysis of Lon proteases in plants highlights independent gene duplication events |
title_full_unstemmed | Comprehensive analysis of Lon proteases in plants highlights independent gene duplication events |
title_short | Comprehensive analysis of Lon proteases in plants highlights independent gene duplication events |
title_sort | comprehensive analysis of lon proteases in plants highlights independent gene duplication events |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6460959/ https://www.ncbi.nlm.nih.gov/pubmed/30590727 http://dx.doi.org/10.1093/jxb/ery440 |
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