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Extracellular peptide Kratos restricts cell death during vascular development and stress in Arabidopsis

During plant vascular development, xylem tracheary elements (TEs) form water-conducting, empty pipes by genetically regulated cell death. Cell death is prevented from spreading to non-TEs by unidentified intercellular mechanisms, downstream of METACASPASE9 (MC9)-mediated regulation of autophagy in T...

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Autores principales: Escamez, Sacha, Stael, Simon, Vainonen, Julia P, Willems, Patrick, Jin, Huiting, Kimura, Sachie, Van Breusegem, Frank, Gevaert, Kris, Wrzaczek, Michael, Tuominen, Hannele
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6460963/
https://www.ncbi.nlm.nih.gov/pubmed/30753577
http://dx.doi.org/10.1093/jxb/erz021
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author Escamez, Sacha
Stael, Simon
Vainonen, Julia P
Willems, Patrick
Jin, Huiting
Kimura, Sachie
Van Breusegem, Frank
Gevaert, Kris
Wrzaczek, Michael
Tuominen, Hannele
author_facet Escamez, Sacha
Stael, Simon
Vainonen, Julia P
Willems, Patrick
Jin, Huiting
Kimura, Sachie
Van Breusegem, Frank
Gevaert, Kris
Wrzaczek, Michael
Tuominen, Hannele
author_sort Escamez, Sacha
collection PubMed
description During plant vascular development, xylem tracheary elements (TEs) form water-conducting, empty pipes by genetically regulated cell death. Cell death is prevented from spreading to non-TEs by unidentified intercellular mechanisms, downstream of METACASPASE9 (MC9)-mediated regulation of autophagy in TEs. Here, we identified differentially abundant extracellular peptides in vascular-differentiating wild-type and MC9-down-regulated Arabidopsis cell suspensions. A peptide named Kratos rescued the abnormally high ectopic non-TE death resulting from either MC9 knockout or TE-specific overexpression of the ATG5 autophagy protein during experimentally induced vascular differentiation in Arabidopsis cotyledons. Kratos also reduced cell death following mechanical damage and extracellular ROS production in Arabidopsis leaves. Stress-induced but not vascular non-TE cell death was enhanced by another identified peptide, named Bia. Bia is therefore reminiscent of several known plant cell death-inducing peptides acting as damage-associated molecular patterns. In contrast, Kratos plays a novel extracellular cell survival role in the context of development and during stress response.
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spelling pubmed-64609632019-04-17 Extracellular peptide Kratos restricts cell death during vascular development and stress in Arabidopsis Escamez, Sacha Stael, Simon Vainonen, Julia P Willems, Patrick Jin, Huiting Kimura, Sachie Van Breusegem, Frank Gevaert, Kris Wrzaczek, Michael Tuominen, Hannele J Exp Bot Research Papers During plant vascular development, xylem tracheary elements (TEs) form water-conducting, empty pipes by genetically regulated cell death. Cell death is prevented from spreading to non-TEs by unidentified intercellular mechanisms, downstream of METACASPASE9 (MC9)-mediated regulation of autophagy in TEs. Here, we identified differentially abundant extracellular peptides in vascular-differentiating wild-type and MC9-down-regulated Arabidopsis cell suspensions. A peptide named Kratos rescued the abnormally high ectopic non-TE death resulting from either MC9 knockout or TE-specific overexpression of the ATG5 autophagy protein during experimentally induced vascular differentiation in Arabidopsis cotyledons. Kratos also reduced cell death following mechanical damage and extracellular ROS production in Arabidopsis leaves. Stress-induced but not vascular non-TE cell death was enhanced by another identified peptide, named Bia. Bia is therefore reminiscent of several known plant cell death-inducing peptides acting as damage-associated molecular patterns. In contrast, Kratos plays a novel extracellular cell survival role in the context of development and during stress response. Oxford University Press 2019-03-15 2019-02-07 /pmc/articles/PMC6460963/ /pubmed/30753577 http://dx.doi.org/10.1093/jxb/erz021 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Papers
Escamez, Sacha
Stael, Simon
Vainonen, Julia P
Willems, Patrick
Jin, Huiting
Kimura, Sachie
Van Breusegem, Frank
Gevaert, Kris
Wrzaczek, Michael
Tuominen, Hannele
Extracellular peptide Kratos restricts cell death during vascular development and stress in Arabidopsis
title Extracellular peptide Kratos restricts cell death during vascular development and stress in Arabidopsis
title_full Extracellular peptide Kratos restricts cell death during vascular development and stress in Arabidopsis
title_fullStr Extracellular peptide Kratos restricts cell death during vascular development and stress in Arabidopsis
title_full_unstemmed Extracellular peptide Kratos restricts cell death during vascular development and stress in Arabidopsis
title_short Extracellular peptide Kratos restricts cell death during vascular development and stress in Arabidopsis
title_sort extracellular peptide kratos restricts cell death during vascular development and stress in arabidopsis
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6460963/
https://www.ncbi.nlm.nih.gov/pubmed/30753577
http://dx.doi.org/10.1093/jxb/erz021
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