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Differential effects of genistein and 8-prenylgenistein on reproductive tissues in immature female mice

Context: We identified an active prenylated derivative of genistein, 8-prenylgenistein (8PG) from Erythrina variegata L. (Leguminosae) and found that 8PG increased osteoprotective effects of genistein in oestrogen-deficient mice. Objective: This study investigated and compared the oestrogenic effect...

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Autores principales: Li, Xiao-Li, Sui, Li, Lin, Fu-Hui, Lian, Yin, Ai, Lian-Zhong, Zhang, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6461073/
https://www.ncbi.nlm.nih.gov/pubmed/30946631
http://dx.doi.org/10.1080/13880209.2019.1590422
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author Li, Xiao-Li
Sui, Li
Lin, Fu-Hui
Lian, Yin
Ai, Lian-Zhong
Zhang, Yan
author_facet Li, Xiao-Li
Sui, Li
Lin, Fu-Hui
Lian, Yin
Ai, Lian-Zhong
Zhang, Yan
author_sort Li, Xiao-Li
collection PubMed
description Context: We identified an active prenylated derivative of genistein, 8-prenylgenistein (8PG) from Erythrina variegata L. (Leguminosae) and found that 8PG increased osteoprotective effects of genistein in oestrogen-deficient mice. Objective: This study investigated and compared the oestrogenic effects of genistein and 8PG on uterus and vagina of immature mice. Materials and methods: Immature female CD-1 mice were orally treated with vehicle (Control, n = 10) or genistein (75 mg/kg, n = 10) or 8PG with low (8PG-L, 75 mg/kg, n = 10) and high dose (8PG-H, 150 mg/kg, n = 10) for 7 consecutive days by intragastric gavage. The uterus and vagina were harvested for histological and molecular measurements. Results: Treatment with genistein and 8PG-H significantly increased uterus index (1.98 ± 0.21 & 1.49 ± 0.16 mg/g) and vagina index (3.83 ± 0.11 & 3.13 ± 0.25 mg/g) as compared to untreated control (uterus, 1.12 ± 0.13 mg/g; vagina, 2.32 ± 0.18 mg/g). Accordingly, both genistein and 8PG-H made vaginal cells keratinized and induced uterine and vaginal hypertrophy associated with the endometrial proliferation. 8PG-L did not affect oestrus cycle and histology of uterus and vagina. Treatment of immature mice with genistein or 8PG-H upregulated protein expression of oestrogen receptor-α (ER-α) and proliferating cell nuclear antigen (PCNA), but 8PG-L did not alter ER-α and PCNA expression in uterus and vagina. Conclusion: This study indicated that 8-prenylgenistein exerted oestrogenic effects in immature female mice. The efficacy and safety of 8-prenylgenistein when applied in improving oestrogen deficiency-induced syndrome requires further elucidation.
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spelling pubmed-64610732019-04-19 Differential effects of genistein and 8-prenylgenistein on reproductive tissues in immature female mice Li, Xiao-Li Sui, Li Lin, Fu-Hui Lian, Yin Ai, Lian-Zhong Zhang, Yan Pharm Biol Short Communication Context: We identified an active prenylated derivative of genistein, 8-prenylgenistein (8PG) from Erythrina variegata L. (Leguminosae) and found that 8PG increased osteoprotective effects of genistein in oestrogen-deficient mice. Objective: This study investigated and compared the oestrogenic effects of genistein and 8PG on uterus and vagina of immature mice. Materials and methods: Immature female CD-1 mice were orally treated with vehicle (Control, n = 10) or genistein (75 mg/kg, n = 10) or 8PG with low (8PG-L, 75 mg/kg, n = 10) and high dose (8PG-H, 150 mg/kg, n = 10) for 7 consecutive days by intragastric gavage. The uterus and vagina were harvested for histological and molecular measurements. Results: Treatment with genistein and 8PG-H significantly increased uterus index (1.98 ± 0.21 & 1.49 ± 0.16 mg/g) and vagina index (3.83 ± 0.11 & 3.13 ± 0.25 mg/g) as compared to untreated control (uterus, 1.12 ± 0.13 mg/g; vagina, 2.32 ± 0.18 mg/g). Accordingly, both genistein and 8PG-H made vaginal cells keratinized and induced uterine and vaginal hypertrophy associated with the endometrial proliferation. 8PG-L did not affect oestrus cycle and histology of uterus and vagina. Treatment of immature mice with genistein or 8PG-H upregulated protein expression of oestrogen receptor-α (ER-α) and proliferating cell nuclear antigen (PCNA), but 8PG-L did not alter ER-α and PCNA expression in uterus and vagina. Conclusion: This study indicated that 8-prenylgenistein exerted oestrogenic effects in immature female mice. The efficacy and safety of 8-prenylgenistein when applied in improving oestrogen deficiency-induced syndrome requires further elucidation. Taylor & Francis 2019-04-04 /pmc/articles/PMC6461073/ /pubmed/30946631 http://dx.doi.org/10.1080/13880209.2019.1590422 Text en © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Li, Xiao-Li
Sui, Li
Lin, Fu-Hui
Lian, Yin
Ai, Lian-Zhong
Zhang, Yan
Differential effects of genistein and 8-prenylgenistein on reproductive tissues in immature female mice
title Differential effects of genistein and 8-prenylgenistein on reproductive tissues in immature female mice
title_full Differential effects of genistein and 8-prenylgenistein on reproductive tissues in immature female mice
title_fullStr Differential effects of genistein and 8-prenylgenistein on reproductive tissues in immature female mice
title_full_unstemmed Differential effects of genistein and 8-prenylgenistein on reproductive tissues in immature female mice
title_short Differential effects of genistein and 8-prenylgenistein on reproductive tissues in immature female mice
title_sort differential effects of genistein and 8-prenylgenistein on reproductive tissues in immature female mice
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6461073/
https://www.ncbi.nlm.nih.gov/pubmed/30946631
http://dx.doi.org/10.1080/13880209.2019.1590422
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