A High-Resolution Luminescent Assay for Rapid and Continuous Monitoring of Protein Translocation across Biological Membranes

Protein translocation is a fundamental process in biology. Major gaps in our understanding of this process arise due the poor sensitivity, low time resolution and irreproducibility of translocation assays. To address this, we applied NanoLuc split-luciferase to produce a new strategy for measuring p...

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Detalles Bibliográficos
Autores principales: Pereira, Gonçalo C., Allen, William J., Watkins, Daniel W., Buddrus, Lisa, Noone, Dylan, Liu, Xia, Richardson, Andrew P., Chacinska, Agnieszka, Collinson, Ian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6461198/
https://www.ncbi.nlm.nih.gov/pubmed/30878481
http://dx.doi.org/10.1016/j.jmb.2019.03.007

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