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In vitro propagation method for production of morphologically and genetically stable plants of different strawberry cultivars

BACKGROUND: As strawberries are susceptible to somaclonal variation when propagated by tissue culture techniques, it is challenging to obtain the true-to-type plants necessary for continuous production of fruits of stable quality. Therefore, we aimed to develop an in vitro propagation method for the...

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Autores principales: Naing, Aung Htay, Kim, Si Hyun, Chung, Mi Young, Park, Soon Ki, Kim, Chang Kil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6461810/
https://www.ncbi.nlm.nih.gov/pubmed/31011361
http://dx.doi.org/10.1186/s13007-019-0421-0
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author Naing, Aung Htay
Kim, Si Hyun
Chung, Mi Young
Park, Soon Ki
Kim, Chang Kil
author_facet Naing, Aung Htay
Kim, Si Hyun
Chung, Mi Young
Park, Soon Ki
Kim, Chang Kil
author_sort Naing, Aung Htay
collection PubMed
description BACKGROUND: As strawberries are susceptible to somaclonal variation when propagated by tissue culture techniques, it is challenging to obtain the true-to-type plants necessary for continuous production of fruits of stable quality. Therefore, we aimed to develop an in vitro propagation method for the production of true-to-type plants of five different strawberry cultivars from meristems cultured in media containing different concentrations of kinetin (Kn). RESULTS: For all the cultivars, shoot induction was successful only in the meristems cultured in the medium without Kn and the medium containing 0.5 mg L(−1) Kn. The shoots obtained from explants cultured in media supplemented with 0.5 mg L(−1) Kn exhibited better plant growth parameters than those cultured in media without Kn and were genetically stable when compared with conventionally propagated plants for all the cultivars. Vegetative and sexual characters and fruit quality attributes observed in the plants derived from meristems cultured on 0.5 mg L(−1) Kn and the conventionally propagated plants were not significantly different when grown for three continuous growing seasons under greenhouse conditions. CONCLUSION: The culture of meristems in the medium containing 0.5 mg L(−1) Kn is suitable for the efficient propagation of true-to-type plants of different strawberry cultivars and continuous production of fruits with stable quality. Hence, we expect that the method presented in this study will be helpful for the commercial production of true-to-type plants generated in vitro for other strawberry cultivars. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13007-019-0421-0) contains supplementary material, which is available to authorized users.
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spelling pubmed-64618102019-04-22 In vitro propagation method for production of morphologically and genetically stable plants of different strawberry cultivars Naing, Aung Htay Kim, Si Hyun Chung, Mi Young Park, Soon Ki Kim, Chang Kil Plant Methods Research BACKGROUND: As strawberries are susceptible to somaclonal variation when propagated by tissue culture techniques, it is challenging to obtain the true-to-type plants necessary for continuous production of fruits of stable quality. Therefore, we aimed to develop an in vitro propagation method for the production of true-to-type plants of five different strawberry cultivars from meristems cultured in media containing different concentrations of kinetin (Kn). RESULTS: For all the cultivars, shoot induction was successful only in the meristems cultured in the medium without Kn and the medium containing 0.5 mg L(−1) Kn. The shoots obtained from explants cultured in media supplemented with 0.5 mg L(−1) Kn exhibited better plant growth parameters than those cultured in media without Kn and were genetically stable when compared with conventionally propagated plants for all the cultivars. Vegetative and sexual characters and fruit quality attributes observed in the plants derived from meristems cultured on 0.5 mg L(−1) Kn and the conventionally propagated plants were not significantly different when grown for three continuous growing seasons under greenhouse conditions. CONCLUSION: The culture of meristems in the medium containing 0.5 mg L(−1) Kn is suitable for the efficient propagation of true-to-type plants of different strawberry cultivars and continuous production of fruits with stable quality. Hence, we expect that the method presented in this study will be helpful for the commercial production of true-to-type plants generated in vitro for other strawberry cultivars. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13007-019-0421-0) contains supplementary material, which is available to authorized users. BioMed Central 2019-04-13 /pmc/articles/PMC6461810/ /pubmed/31011361 http://dx.doi.org/10.1186/s13007-019-0421-0 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Naing, Aung Htay
Kim, Si Hyun
Chung, Mi Young
Park, Soon Ki
Kim, Chang Kil
In vitro propagation method for production of morphologically and genetically stable plants of different strawberry cultivars
title In vitro propagation method for production of morphologically and genetically stable plants of different strawberry cultivars
title_full In vitro propagation method for production of morphologically and genetically stable plants of different strawberry cultivars
title_fullStr In vitro propagation method for production of morphologically and genetically stable plants of different strawberry cultivars
title_full_unstemmed In vitro propagation method for production of morphologically and genetically stable plants of different strawberry cultivars
title_short In vitro propagation method for production of morphologically and genetically stable plants of different strawberry cultivars
title_sort in vitro propagation method for production of morphologically and genetically stable plants of different strawberry cultivars
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6461810/
https://www.ncbi.nlm.nih.gov/pubmed/31011361
http://dx.doi.org/10.1186/s13007-019-0421-0
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