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In Vitro Anti-Biofilm Activities of Citral and Thymol Against Candida tropicalis

Candida tropicalis is an emerging non-albicans Candida species which is pathogenic to the immune-compromised humans, especially in tropical countries, including India. The acquired resistance of Candida species towards antifungal therapies is of major concern. Moreover, limited efficacy and dosage c...

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Autores principales: Chatrath, Apurva, Gangwar, Rashmi, Kumari, Poonam, Prasad, Ramasare
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6462931/
https://www.ncbi.nlm.nih.gov/pubmed/30717454
http://dx.doi.org/10.3390/jof5010013
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author Chatrath, Apurva
Gangwar, Rashmi
Kumari, Poonam
Prasad, Ramasare
author_facet Chatrath, Apurva
Gangwar, Rashmi
Kumari, Poonam
Prasad, Ramasare
author_sort Chatrath, Apurva
collection PubMed
description Candida tropicalis is an emerging non-albicans Candida species which is pathogenic to the immune-compromised humans, especially in tropical countries, including India. The acquired resistance of Candida species towards antifungal therapies is of major concern. Moreover, limited efficacy and dosage constraint of synthetic drugs have indicated the prerequisite of finding new and natural drugs for treatment. In the present study, we have compared the influence of citral and thymol on C. tropicalis and its biofilm along with expression levels of certain antifungal tolerance genes. The antifungal and anti-biofilm activities of the both were studied using 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide sodium salt (XTT) reduction assay, field emission scanning electron microscope (FE-SEM) and confocal laser scanning microscope (CLSM) and real-time reverse transcription polymerase chain reaction (RT-PCR) analysis. Citral and thymol have damaged the cells with distorted surface and less viability. Quantitative real-time PCR analysis showed augmented expression of the cell membrane biosynthesis genes including ERG11/CYT450 against citral and the cell wall related tolerance genes involving CNB1 against thymol thus, depicting their differential mode of actions.
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spelling pubmed-64629312019-04-17 In Vitro Anti-Biofilm Activities of Citral and Thymol Against Candida tropicalis Chatrath, Apurva Gangwar, Rashmi Kumari, Poonam Prasad, Ramasare J Fungi (Basel) Article Candida tropicalis is an emerging non-albicans Candida species which is pathogenic to the immune-compromised humans, especially in tropical countries, including India. The acquired resistance of Candida species towards antifungal therapies is of major concern. Moreover, limited efficacy and dosage constraint of synthetic drugs have indicated the prerequisite of finding new and natural drugs for treatment. In the present study, we have compared the influence of citral and thymol on C. tropicalis and its biofilm along with expression levels of certain antifungal tolerance genes. The antifungal and anti-biofilm activities of the both were studied using 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide sodium salt (XTT) reduction assay, field emission scanning electron microscope (FE-SEM) and confocal laser scanning microscope (CLSM) and real-time reverse transcription polymerase chain reaction (RT-PCR) analysis. Citral and thymol have damaged the cells with distorted surface and less viability. Quantitative real-time PCR analysis showed augmented expression of the cell membrane biosynthesis genes including ERG11/CYT450 against citral and the cell wall related tolerance genes involving CNB1 against thymol thus, depicting their differential mode of actions. MDPI 2019-02-03 /pmc/articles/PMC6462931/ /pubmed/30717454 http://dx.doi.org/10.3390/jof5010013 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chatrath, Apurva
Gangwar, Rashmi
Kumari, Poonam
Prasad, Ramasare
In Vitro Anti-Biofilm Activities of Citral and Thymol Against Candida tropicalis
title In Vitro Anti-Biofilm Activities of Citral and Thymol Against Candida tropicalis
title_full In Vitro Anti-Biofilm Activities of Citral and Thymol Against Candida tropicalis
title_fullStr In Vitro Anti-Biofilm Activities of Citral and Thymol Against Candida tropicalis
title_full_unstemmed In Vitro Anti-Biofilm Activities of Citral and Thymol Against Candida tropicalis
title_short In Vitro Anti-Biofilm Activities of Citral and Thymol Against Candida tropicalis
title_sort in vitro anti-biofilm activities of citral and thymol against candida tropicalis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6462931/
https://www.ncbi.nlm.nih.gov/pubmed/30717454
http://dx.doi.org/10.3390/jof5010013
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