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A Culture-Independent Analysis of the Microbiota of Female Interstitial Cystitis/Bladder Pain Syndrome Participants in the MAPP Research Network

We surveyed urine microbiota of females diagnosed with interstitial cystitis/bladder pain syndrome (IC/BPS) and matched control participants enrolled in the National Institutes of Health (NIH) Multidisciplinary Approach to the Study of Chronic Pelvic Pain (MAPP) Research Network using the culture-in...

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Autores principales: Nickel, J. Curtis, Stephens-Shields, Alisa J., Landis, J. Richard, Mullins, Chris, van Bokhoven, Adrie, Lucia, M. Scott, Henderson, Jeffrey P., Sen, Bhaswati, Krol, Jaroslaw E., Ehrlich, Garth D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6462969/
https://www.ncbi.nlm.nih.gov/pubmed/30917614
http://dx.doi.org/10.3390/jcm8030415
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author Nickel, J. Curtis
Stephens-Shields, Alisa J.
Landis, J. Richard
Mullins, Chris
van Bokhoven, Adrie
Lucia, M. Scott
Henderson, Jeffrey P.
Sen, Bhaswati
Krol, Jaroslaw E.
Ehrlich, Garth D.
author_facet Nickel, J. Curtis
Stephens-Shields, Alisa J.
Landis, J. Richard
Mullins, Chris
van Bokhoven, Adrie
Lucia, M. Scott
Henderson, Jeffrey P.
Sen, Bhaswati
Krol, Jaroslaw E.
Ehrlich, Garth D.
author_sort Nickel, J. Curtis
collection PubMed
description We surveyed urine microbiota of females diagnosed with interstitial cystitis/bladder pain syndrome (IC/BPS) and matched control participants enrolled in the National Institutes of Health (NIH) Multidisciplinary Approach to the Study of Chronic Pelvic Pain (MAPP) Research Network using the culture-independent methodology. Midstream urine specimens were analyzed with the Plex-ID molecular diagnostic platform that utilizes polymerase chain reaction–electrospray ionization–time-of-flight–mass spectrometry (PCR-ESI-TOF MS) to provide a comprehensive identification of bacterial and select fungal species. IC/BPS and control participants were evaluated for differences (presence, diversity, and abundance) in species and genus. Urine specimens obtained from 181 female IC/BPS and 182 female control participants detected a total of 92 species (41 genera). Mean (SD) species count was 2.49 (1.48) and 2.30 (1.28) among IC/BPS and control participants, respectively. Overall species composition did not significantly differ between IC/BPS and control participants at any level (p = 0.726 species level, p = 0.222 genus level). IC/BPS participants urine trended to an overabundance of Lactobacillus gasseri (p = 0.09) detected but had a lower prevalence of Corynebacterium compared with control participants (p = 0.002). The relative abundance data analysis mirrored the prevalence data differences with no significant differences in most species or genus abundance other than Lactobacillus gasseri and Corynebacterium (p = 0.08 and p = 0.001, respectively). No cause and/or effect conclusion can be drawn from this observation, but it suggests that a more comprehensive evaluation (vaginal, bowel, catheterized bladder and/or tissue-based specimens) of the lower urinary tract microbiota in IC/BPS patients is warranted.
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spelling pubmed-64629692019-04-19 A Culture-Independent Analysis of the Microbiota of Female Interstitial Cystitis/Bladder Pain Syndrome Participants in the MAPP Research Network Nickel, J. Curtis Stephens-Shields, Alisa J. Landis, J. Richard Mullins, Chris van Bokhoven, Adrie Lucia, M. Scott Henderson, Jeffrey P. Sen, Bhaswati Krol, Jaroslaw E. Ehrlich, Garth D. J Clin Med Article We surveyed urine microbiota of females diagnosed with interstitial cystitis/bladder pain syndrome (IC/BPS) and matched control participants enrolled in the National Institutes of Health (NIH) Multidisciplinary Approach to the Study of Chronic Pelvic Pain (MAPP) Research Network using the culture-independent methodology. Midstream urine specimens were analyzed with the Plex-ID molecular diagnostic platform that utilizes polymerase chain reaction–electrospray ionization–time-of-flight–mass spectrometry (PCR-ESI-TOF MS) to provide a comprehensive identification of bacterial and select fungal species. IC/BPS and control participants were evaluated for differences (presence, diversity, and abundance) in species and genus. Urine specimens obtained from 181 female IC/BPS and 182 female control participants detected a total of 92 species (41 genera). Mean (SD) species count was 2.49 (1.48) and 2.30 (1.28) among IC/BPS and control participants, respectively. Overall species composition did not significantly differ between IC/BPS and control participants at any level (p = 0.726 species level, p = 0.222 genus level). IC/BPS participants urine trended to an overabundance of Lactobacillus gasseri (p = 0.09) detected but had a lower prevalence of Corynebacterium compared with control participants (p = 0.002). The relative abundance data analysis mirrored the prevalence data differences with no significant differences in most species or genus abundance other than Lactobacillus gasseri and Corynebacterium (p = 0.08 and p = 0.001, respectively). No cause and/or effect conclusion can be drawn from this observation, but it suggests that a more comprehensive evaluation (vaginal, bowel, catheterized bladder and/or tissue-based specimens) of the lower urinary tract microbiota in IC/BPS patients is warranted. MDPI 2019-03-26 /pmc/articles/PMC6462969/ /pubmed/30917614 http://dx.doi.org/10.3390/jcm8030415 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nickel, J. Curtis
Stephens-Shields, Alisa J.
Landis, J. Richard
Mullins, Chris
van Bokhoven, Adrie
Lucia, M. Scott
Henderson, Jeffrey P.
Sen, Bhaswati
Krol, Jaroslaw E.
Ehrlich, Garth D.
A Culture-Independent Analysis of the Microbiota of Female Interstitial Cystitis/Bladder Pain Syndrome Participants in the MAPP Research Network
title A Culture-Independent Analysis of the Microbiota of Female Interstitial Cystitis/Bladder Pain Syndrome Participants in the MAPP Research Network
title_full A Culture-Independent Analysis of the Microbiota of Female Interstitial Cystitis/Bladder Pain Syndrome Participants in the MAPP Research Network
title_fullStr A Culture-Independent Analysis of the Microbiota of Female Interstitial Cystitis/Bladder Pain Syndrome Participants in the MAPP Research Network
title_full_unstemmed A Culture-Independent Analysis of the Microbiota of Female Interstitial Cystitis/Bladder Pain Syndrome Participants in the MAPP Research Network
title_short A Culture-Independent Analysis of the Microbiota of Female Interstitial Cystitis/Bladder Pain Syndrome Participants in the MAPP Research Network
title_sort culture-independent analysis of the microbiota of female interstitial cystitis/bladder pain syndrome participants in the mapp research network
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6462969/
https://www.ncbi.nlm.nih.gov/pubmed/30917614
http://dx.doi.org/10.3390/jcm8030415
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